Association of the leukemia inhibitory factor gene mutation and the antiphospholipid antibodies in the peripheral blood of infertile women

To characterize the impact of the potentially functional mutation — the G to A transition at the position 3400 of the leukemia inhibitory factor (LIF; a pluripotent cytokine that plays a central role in the control of the embryo implantation) gene that leads to the exchange of valine with methionine at codon 64 we evaluated the association of the LIF gene mutation and the levels of antiphospolipid antibodies (aPLs) in the peripheral blood of infertile women (the aPLs examination was part of our routine immunological test during the infertility check-up). Eight infertile mutation-positive women were diagnosed with idiopathic infertility (n=5) and endometriosis (n=3) and their levels of aPLs in serum were compared with 115 infertile women without any LIF gene mutation. Enzyme-linked immunosorbent assay was used for the detection of seven antiphospholipid antibodies; the results were statistically assessed by the Fisher’s 2 by 2 exact test to evaluate the association of the LIF gene mutations and aPLs in serum of infertile patients. The presence of aPLs was significantly higher in our study group (100 %) than in 30 % of aPLs-positives in control infertile patients (p=0.0035) which indicates that the aPLs are elevated in women with LIF gene mutations. Similar content being viewed by others Abbreviations - aPLs: - antiphospholipid antibodies - ELISA: - enzyme-linked immunosorbent assay - FCS: - fetal calf serum - IVF: - in vitro fertilization - LIF: - leukemia inhibitory factor - LIFR: - LIF receptor - NK: - natural killer (cells) - ph-Ser: - l-phosphatidyl-serine - SD: - standard deviation - TGGE: - temperature gradient gel electrophoresis

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Ulčová-Gallová) and theInstitutional Research Concept of theInstitute of Microbiology, Academy of Sciences of the Czech Republic (AV 0Z 50200510; P. Šíma). Rights and permissions About this article Cite this article Králíčková, M., Ulčová-Gallová, Z., Šíma, R. et al. Association of the leukemia inhibitory factor gene mutation and the antiphospholipid antibodies in the peripheral blood of infertile women. Folia Microbiol 52, 543–548 (2007). https://doi.org/10.1007/BF02932117 Received: Revised: Issue date: DOI: https://doi.org/10.1007/BF02932117