TGF-βI increases the expression levels of OCT4 and migration-related genes in human endometrial cells.

Heng-Kien Au (837971), Jui-Hung Chang (837972), Yu-Chih Wu (837973), Yung-Che Kuo (175057), Yu-Hsi Chen (837974), Wei-Chin Lee (837975), Te-Sheng Chang (837976), Pei-Chi Lan (837977), Hung-Chih Kuo (554958), Kha-Liang Lee (837978), Mei-Tsu Lee (837979), Chii-Ruey Tzeng (837980), Yen-Hua Huang (409275)
2015 · doi:10.1371/journal.pone.0145256.g003 · W6923375587
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⚙ AI-generated summary by gemini-2.5-flash-lite, 2026-07-14 ⓘ

This study found that TGF-β1 treatment increased OCT4 mRNA and protein expression, as well as SNAIL and N-CADHERIN gene expression in human endometrial cells.

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⚙ AI-generated deep summary by claude@2026-07, 2026-07-14 · read from full text ⓘ

This study investigated how transforming growth factor beta 1 (TGF-βI) affects pluripotency-associated OCT4 and migration-related gene expression in human endometrial and endometriosis-derived cells, using primary endometriotic stromal cells and endometrial cell lines (RL95-2 and HEC1A). Cells were treated with cytokines/growth factors for 24 hours, and OCT4 mRNA expression was quantified by qRT-PCR, with OCT4, SNAIL, and N-cadherin assessed across a range of TGF-β doses and OCT4 protein levels measured by Western blot. The key finding was that TGF-βI increased OCT4 expression along with migration-related genes, paralleling changes at both mRNA and protein levels. The work’s main limitation is that it reports expression changes under in vitro treatment conditions rather than functional migration outcomes. This paper is centrally about endometriosis — it specifically tests TGF-βI-induced OCT4 and migration-related gene upregulation in human endometriotic stromal cells.

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Abstract

(A) The relative expression folds of OCT4 mRNA in human endometriotic stromal cells under different cytokine or growth factor treatments were normalized to the untreated control group. The primary human endometriotic stromal cells were treated with IL-6 (50 ng/mL), IGF-I (50 ng/mL), IL-1β (50 ng/mL), TNF-α (50 ng/mL), and TGF-βI (1 ng/mL) for 24 hours. (B) The relative transcriptional levels of OCT4, SNAIL, and N-CADHERIN (N-CAD) in the RL95-2, HEC1A, and primary endometriotic stroma cells under TGF-β treatment (0, 0, 05, 0.1, 0.5, 1, and 5 ng/mL) were determined using quantitative real-time PCR. Data are representative of at least 3 independent experiments. (C) The relative protein levels of primary human endometriotic stromal cells in (B) were analyzed using Western blot analysis. *P < .05, **P < .01, and ***P < .001 by t test.
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TGF-βI Regulates Cell Migration through Pluripotent Transcription Factor OCT4 in Endometriosis Fig 3 TGF-βI increases the expression levels of OCT4 and migration-related genes in human endometrial cells. (A) The relative expression folds of OCT4 mRNA in human endometriotic stromal cells under different cytokine or growth factor treatments were normalized to the untreated control group. The primary human endometriotic stromal cells were treated with IL-6 (50 ng/mL), IGF-I (50 ng/mL), IL-1β (50 ng/mL), TNF-α (50 ng/mL), and TGF-βI (1 ng/mL) for 24 hours. (B) The relative transcriptional levels of OCT4, SNAIL, and N-CADHERIN (N-CAD) in the RL95-2, HEC1A, and primary endometriotic stroma cells under TGF-β treatment (0, 0, 05, 0.1, 0.5, 1, and 5 ng/mL) were determined using quantitative real-time PCR. Data are representative of at least 3 independent experiments. (C) The relative protein levels of primary human endometriotic stromal cells in (B) were analyzed using Western blot analysis. *P < .05, **P < .01, and ***P < .001 by t test.

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