Stimulatory Effects of Prostaglandins upon Endometrial Alkaline Phosphatase Activity during the Decidual Cell Reaction in the Rat1

Gayle M. Yee, T. G. Kennedy
In: Biology of Reproduction · 1988 · vol. 38(5) , pp. 1129–1136 · doi:10.1095/biolreprod38.5.1129 · PMID:3408781 · W2101534724
article OA: bronze CC0 ⤵ 2 in-corpus citations
📄 Open PDF View on OpenAlex View on PubMed View at publisher
⚙ AI-generated summary by gemini-2.5-flash-lite, 2026-06-13 ⓘ

Indomethacin inhibited endometrial alkaline phosphatase activity during rat deciduogenesis, but exogenous prostaglandins PGE2 and PGF2 alpha restored this activity.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

Abstract

An early event of the decidual cell reaction in response to either natural or artificial deciduogenic stimuli is an increase in the activity of alkaline phosphatase in endometrial stromal cells. Since it is known that prostaglandins have a stimulatory role in other events of the decidual cell reaction, this study addressed whether prostaglandins have any role in the increase in alkaline phosphatase activity. Mature ovariectomized rats were sensitized for the decidual cell reaction with s.c. injections of estrogen and progesterone. To investigate the effect of inhibition of endogenous prostaglandin synthesis, rats received indomethacin (s.c. and via intrauterine infusion) or the vehicles sesame oil and phosphate-buffered saline with gelatin. Biochemical measurement of endometrial alkaline phosphatase activity and protein content revealed that, compared to the controls, indomethacin had no significant effect at 24 h after the initiation of infusion. At 48 h, unit activity (activity per unit protein) and total activity were substantially lower in indomethacin-treated than in vehicle-treated rats. At 72 h, there was a less dramatic difference between the unit activities of the two groups; however, there was a 10-fold difference between the total measureable activities of the two groups. Infusion of exogenous prostaglandins PGE2 or PGF2 alpha into rats treated with indomethacin overrode the inhibitory effect of indomethacin upon the increase in alkaline phosphatase activity. The dose-response relationship indicated that both PGE2 and PGF2 alpha have the same efficacy. In rats that had been treated similarly, localization of uterine alkaline phosphatase activity by histochemistry demonstrated a high correlation between the histochemical and biochemical data.(ABSTRACT TRUNCATED AT 250 WORDS)

My notes (saved in your browser only)

Citation neighborhood (sparse)

Too few in-corpus citations on either side for a chart; here are the lists.

Cites (3)

Cited by (2)

References (25)

Cited by (2)

Source provenance

openalex
last seen: 2026-06-10T17:14:06.276822+00:00
License: CC0 · commercial use OK