Establishment of endometriosis model in allogeneic Sprague-Dawley rats

To explore the feasibility of the establishment of endometriosis (EMS) model in allogeneic rats by abdominal subcutaneous implantation.

Choose 48 healthy, unmated and in estrus (normal estrous cycle ≥ 2) female Sprague-Dawley (SD) rats as the research object. According to random number table, 48 SD rats were divided into donor rats (n=24) and recipient rats (n=24). When the SD rats in estrus, we sutured the donor rats uterine tissue to the abdominal subcutaneous of recipient rats, and removed the ectopic lesion tissue 3 weeks after surgery, then observe histopathologic findings under an optical microscope.

All of those 48 SD rats had regular estrous cycles, there were 4 estrous cycles which were proestrus, estrus, metestrus, and diestrus, each period lasted 4-5 d. Twenty-four SD rats (recipient rats) were alive, the successful rate of allogeneic abdominal subcutaneous implantation of SD rats was 66.7%(16/24), in which 16 SD rats' abdomen could see dome projection, which was cystic and solid, non-active, and cut the skin open, we can see translucent dome cystic proliferator, texture soft, clear liquid accumulation in the capsule. The average volume was (34.5±19.8) mm3. Ectopic lesion tissue was observed through low magnification optical microscope (×100), and we could see ectopic lesions grew into a cavity-like structure. Through we could see endometrial epithelial cells, stromal cells and the formation of a small amount of glandular under high magnification optical microscope (×400). Tissue pathology was similar to human EMS.

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