{"paper_id":"194bbbc4-0254-4b4f-8ba3-53bd0cc272c0","body_text":"中华妇幼临床医学杂志(电子版) ›› 2015, Vol. 11 ›› Issue (03) : 388 -392. doi: 10.3877/cma.j.issn.1673-5250.2015.03.022\n所属专题： 文献；\n论著\n同种异体Sprague-Dawley大鼠子宫内膜异位症动物模型的建立\n- 1. 256600 山东省滨州医学院附属医院\n- 1. Department of Obstetrics and Gynecology, Affiliated Hospital to Binzhou Medical University, Binzhou 256603, Shandong Province, China\n目的\n探讨采用皮下移植法建立大鼠同种异体子宫内膜异位症(EMS)模型的可行性。\n方法\n选择健康、未交配且处于动情期(正常动情周期≥2个)的雌性Sprague-Dawley(SD)大鼠48只为研究对象。按照随机数字表法，将48只SD大鼠平均分为供鼠(n＝24)和受鼠(n＝24)。于SD大鼠处于动情期时将供鼠子宫组织缝合于受鼠腹部皮下，并于手术后3周取出异位病灶组织，于光学显微镜下观察异位病灶组织的形态学及组织病理学结果。\n结果\n48只SD大鼠均有规律的4个动情周期，分别为动情前期、动情期、动情后期、动情间期，各个周期分别为4～5 d。24只SD大鼠(受鼠)均存活，同种异体SD大鼠皮下移植法成模率为66.7%(16/24)，其中16只SD大鼠腹部肉眼可见球型凸起，触之呈囊实性，无活动性，切开皮肤可见透亮的球型囊状增生物，质地软，囊内有清亮液体聚积，平均体积为(34.5±19.8)mm3，异位病灶组织于光学显微镜低倍镜(×100)下可见异位病灶生长成一个腔样结构，于高倍镜下(×400)可见内膜上皮细胞、间质细胞及少量腺体形成，组织病理学表现与人EMS相似。\n结论\n通过皮下移植法建立的同种异体SD大鼠EMS模型的异位病灶组织的组织病理学改变与人EMS类似，可以作为EMS研究的动物模型。\nObjective\nTo explore the feasibility of the establishment of endometriosis (EMS) model in allogeneic rats by abdominal subcutaneous implantation.\nMethods\nChoose 48 healthy, unmated and in estrus (normal estrous cycle ≥ 2) female Sprague-Dawley (SD) rats as the research object. According to random number table, 48 SD rats were divided into donor rats (n=24) and recipient rats (n=24). When the SD rats in estrus, we sutured the donor rats uterine tissue to the abdominal subcutaneous of recipient rats, and removed the ectopic lesion tissue 3 weeks after surgery, then observe histopathologic findings under an optical microscope.\nResults\nAll of those 48 SD rats had regular estrous cycles, there were 4 estrous cycles which were proestrus, estrus, metestrus, and diestrus, each period lasted 4-5 d. Twenty-four SD rats (recipient rats) were alive, the successful rate of allogeneic abdominal subcutaneous implantation of SD rats was 66.7%(16/24), in which 16 SD rats' abdomen could see dome projection, which was cystic and solid, non-active, and cut the skin open, we can see translucent dome cystic proliferator, texture soft, clear liquid accumulation in the capsule. The average volume was (34.5±19.8) mm3. Ectopic lesion tissue was observed through low magnification optical microscope (×100), and we could see ectopic lesions grew into a cavity-like structure. Through we could see endometrial epithelial cells, stromal cells and the formation of a small amount of glandular under high magnification optical microscope (×400). Tissue pathology was similar to human EMS.\nConclusions\nThe tissue pathology changes of ectopic endometrial in EMS model of allogeneic SD rats by subcutaneous transplantation were similar to human EMS, EMS SD rats model can be used in the studies on EMS.\n| [1] |\n谢辛，苟文丽．妇产科学.8版[M]．北京：人民卫生出版社，2013：268–274.\n|\n| [2] |\n姚红梅，郭静，石秀玲. VEGF CD44v6 MMP-9在子宫内膜异位症组织中的表达及临床意义[J]. 2013, 36(2): 124–126.\n|\n| [3] |\nGiudice LC. Clinical practice. Endometriosis[J]. N Engl J Med, 2010, 362(25): 2389–2398.\n|\n| [4] |\nLim HJ, Wang H．Uterine disorders and pregnancy complications: insights from mouse models[J]. J Clin Invest, 2010, 120(4): 1004–1015.\n|\n| [5] |\nNgô C, Nicco C, Leconte M, et al. Protein kinase inhibitors can control the progression of endometriosis in vitro and in vivo[J]. J Pathol, 2010, 222(2): 148–157.\n|\n| [6] |\nKhoufache K, Bondza PK, Harir N, et al. Soluble human IL-1 receptor type 2 inhibits ectopic endometrial tissue implantation and growth: identification of a novel potential target for endometriosis treatment[J]. Am J Pathol, 2012, 181(4): 1197–1205.\n|\n| [7] |\nLeconte M, Nicco C, Ngô C, et al. The mTOR/AKT inhibitor temsirolimus prevents deep infiltrating endometriosis in mice[J]. Am J Pathol, 2011, 179(2): 880–889.\n|\n| [8] |\nUchida M, Kobayashi O. Sequential observation of implanted endometriosis by laparoscopy in rats: correlation between the prevalence rate and the estrous cycle[J]. J Pharmacol Sci, 2013, 121(4): 299–304.\n|\n| [9] |\n王洁，吴素慧，尚海霞. 性成熟雌性SD大鼠动情周期的观察[J]. 当代医学，2013, 19(28): 25–26.\n|\n| [10] |\nUchida M, Kobayashi O. Sequential observation of implanted endometriosis by laparoscopy in rats: correlation between the prevalence rate and the estrous cycle[J]. J Pharmacol Sci, 2013, 121(4): 299–304.\n|\n| [11] |\nFeng D, Menger MD, Laschke MW. Vascular disrupting effects of combretastatin A4 phosphate on murine endometriotic lesions[J]. Fertil Steril, 2013, 100(5): 1459–1467.\n|\n| [12] |\nLee HJ, Lee HJ, Lee JM, et al. Ultrasmall superparamagnetic iron oxides enhanced MR imaging in rats with experimentally induced endometriosis[J]. Magn Reson Imaging, 2012, 30(6): 860–868.\n|\n| [13] |\nSchwager K, Bootz F, Imesch P, et al. The antibody-mediated targeted delivery of interleukin-10 inhibits endometriosis in a syngeneic mouse model[J]. Hum Reprod, 2011, 26(9): 2344–2352\n|\n| [14] |\n周玲，钱志红，任琼珍. 皮下注射子宫内膜法建立Lewis大鼠腹壁子宫内膜异位症模型[J]. 江苏医药，2012, 38(13): 1513–1515.\n|\n| [15] |\nHuniadi CA, Pop OL, Antal TA, et al. The effects of ulipristal on Bax/Bcl-2, cytochrome c, Ki-67 and cyclooxygenase-2 expression in a rat model with surgically induced endometriosis[J]. Eur J Obstet Gynecol Reprod Biol, 2013, 169(2): 360–365.\n|\n| [16] |\nAltan ZM, Denis D, Kagan D, et al. A long-acting tumor necrosis factor alpha-binding protein demonstrates activity in both in vitro and in vivo models of endometriosis[J]. J Pharmacol Exp Ther, 2010, 334(2): 460–466.\n|\n| [17] |\n崔阳阳，孙伟伟，赵瑞华. 子宫内膜异位症动物模型研究进展[J]. 中国实验动物学报，2013, 21(5): 86–89.\n|\n| [18] |\n彭艳，何援利. 大鼠子宫内膜异位症动物模型建立方法的比较[J]. 重庆医学，2012, 41(25): 2619–2620.\n|\n| [19] |\nPullen N, Birch CL, Douglas GJ, et al. The translational challenge in the development of new and effective therapies for endometriosis: a review of confidence from published preclinical efficacy studies[J]. Hum Reprod Update, 2011, 17(6): 791–802.\n|\n| [20] |\nGrechukhina O, Petracco R, Popkhadze S, et al. A polymorphism in a let-7 microRNA binding site of KRAS in women with endometriosis[J]. 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