Interaction of sperm with endometrium can regulate genes involved in endometrial receptivity pathway in mice: An experimental study.

Marziyeh Ajdary, Zahra Zandieh, Amjadi FS, Keyhanfar F, Mehdizadeh M, Reza Aflatoonian
International journal of reproductive biomedicine · 2020 · doi:10.18502/ijrm.v13i10.7765 · PMID:33134794 · PMC7569714
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Interaction between sperm and endometrium in mice upregulated mRNA levels of LIF, LIFR, MUC1, VEGF, EGF, and FGF2, indicating seminal plasma regulates genes associated with the endometrial receptivity pathway.

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This experimental study investigated the influence of sperm interaction with the endometrium on gene expression related to endometrial receptivity using a mouse model. Researchers compared female mice mated with vasectomized males against those mated with non-vasectomized males, analyzing endometrial tissue via quantitative real-time PCR. The results demonstrated that seminal plasma significantly upregulated the mRNA levels of LIF, MUC1, VEGF, EGF, and FGF2 in the endometrium, while other genes such as HOXA10 showed decreased expression. These findings indicate that seminal plasma actively regulates the genetic environment of the endometrium to facilitate embryo implantation. This paper is centrally about endometriosis — specifically laparoscopic excision of deep infiltrating lesions.

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Abstract

BackgroundMany researchers consider implantation and endometrial receptivity as pertinent issues in reproductive science. Although, several experiments have been performed and their results evaluated, yet there is no confirmed evidence about the related factors and the role of sperm in endometrial receptivity.ObjectiveTo investigate the effect of the sperm-endometrium interaction in regulating genes involved in the endometrial receptivity pathway.Materials and methodsIn this experimental study, 10 male and 30 female NMRI mice were included, and half of the male cases were vasectomized. The subjects were divided into two groups as follows; group 1 (case) comprised of 15 females mated with 5 non-vasectomized male mice, while group 2 (control) consisted of 15 females mated with 5 vasectomized males. Cases were sacrificed and assessed after 36 hr and the endometrial tissue was extracted and kept at -80°C until the next use. The expression of the endometrial receptivity pathway genes, including VEGF, HBEGF, FGF2, EGF, LIF, LIFR, HOXA10, MUC1, PGR, and CSF, was examined in both groups. For statistical analysis, an independent samples test (Mean ± SD) was used.ResultsThe mRNA levels of LIF (p = 0.045), LIFR (p = 0.040), MUC1 (p = 0.032), VEGF (p = 0.022), EFG (p = 0.035), and FGF2 (p = 0.040) were significantly upregulated in the case group compared with the control group.ConclusionFinally, seminal plasma was observed to be effective in expressing the involved genes in the successful implantation pathway, including LIF, LIFR, MUC1, VEGF, EGF, and FGF2.
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Finally, it was found that the seminal plasma is effective in expressing the involved genes in implantation in the in vivo model. In fact, seminal plasma increases endometrial receptivity prior to an embryo transfer. Seminal plasma induces the expression of LIF, LIFR, MUC1, VEGF, EGF, and FGF2 genes that increase stromal cell survival and endometrial tissue proliferation before an implantation. According to the obtained results in this study, seminal plasma can be introduced as an auxiliary and effective factor in successful implantation. Taken together, our findings and those of others suggest that the effects of seminal plasma on the endometrium and pregnancy should be investigated in an in vivo/in vitro model for confirmation of these results.

Coi Statement

The authors declare that there is no competing interest.

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