The effect of exogenous estradiol treatment on the mRNA expression of vascular endothelial growth factor and its receptors in cultured human oviduct mucosal cells

Purpose: To evaluate the responses of cultured oviduct mucosal cells to exogenous estradiol treatment in regulating the mRNA expression of vascular endothelial growth factor (VEGF) and its receptors.

The mucosal layer of the ampullary regions of the human oviduct was isolated and cultured with (study groups) or without (control group) the addition of exogenous estradiol in five different concentrations. Semiquantitative reverse-transcriptase-polymerase chain reaction was performed on the oviduct mucosal cells before and after the 6-day culture.

There were no significant differences in the mRNA expression of VEGF and its receptors, both KDR and flt-1, between the five study groups and the control group.

The mRNA expression of VEGF and its receptors is not altered by exogenous estradiol treatment in cultured oviduct. This helps to explain the mechanism of temporal regulation of VEGF in human oviduct, which reaches the peak level in the peri-ovulatory stage when both the serum estradiol and gonadotropins concentrations are high. Similar content being viewed by others

Gabler C, Einspanier A, Schams D, Einspanier R: Expression of vascular endothelial growth factor (VEGF) and its corresponding receptors (flt-1 and flk-1) in the bovine oviduct. Mol Reprod Dev 1999;53(4):376–383 Lam P, Briton-Jones C, Cheung C, Lok I, Yuen P, Cheung L, et al: Vascular endothelial growth factor in the human oviduct: Localization and regulation of messenger RNA expression in vivo. Biol Reprod 2003;68(5):1870–1876 Lam P, Briton-Jones C, Cheung C, Lok I, Cheung L, Haines C: In vivo Regulation of mRNA expression of vascular endothelial growth factor receptors (KDR & flt-1) in the human oviduct. Fertil Steril 2004;81(2):416–423 Lam P, Briton-Jones C, Cheung C, Cheung L, Haines C: Up-regulation of mRNA expression of vascular endothelial growth factor and its receptors by exogenous human chorionic gonadotropin in cultured oviduct mucosal cells. Fertil Steril 2004;82(6):1708–1710 Shifren JL, Tseng JF, Zaloudek CJ, Ryan IP, Meng YG, Ferrara N, et al: Ovarian steroid regulation of vascular endothelial growth factor in the human endometrium: Implications for angiogenesis during the menstrual cycle and in the pathogenesis of endometriosis. J Clin Endocrinol Metab 1996;81(8):3112–3118 Sugino N, Kashida S, Karube-Harada A, Takiguchi S, Kato H: Expression of vascular endothelial growth factor (VEGF) and its receptors in human endometrium throughout the menstrual cycle and in early pregnancy. Reproduction 2002;123(3):379–387 Comer M, Leese H, Southgate J: Induction of a differentiated ciliated cell phenotype in primary cultures of Fallopian tube epithelium. Hum Reprod 1998;13(11):3114–3120 Briton-Jones C, Lok I, Cheung C, Chiu T, Cheung L, Haines C: Estradiol regulation of oviductin/oviduct-specific glycoprotein messenger ribonucleic acid expression in human oviduct mucosal cells in vitro. Fertil Steril 2004;81(Suppl 1):749–756 Briton-Jones C, Lok I, Chiu T, Cheung L, Haines C: Human chorionic gonadotropin and 17-beta estradiol regulation of human oviductin/oviduct specific glycoprotein mRNA expression in vitro. Fertil Steril 2003;80 Suppl(2):720–726 Kumar N, Richer J, Owen G, Litman E, Horwitz K, Leslie K: Selective down-regulation of progesterone receptor isoform B in poorly differentiated human endometrial cancer cells: Implications for unopposed estrogen action. Cancer Res 1998;58(9):1860–1865 Matsuzaki S, Fukaya T, Uehara S, Murakami T, Sasano H, Yajima A: Characterization of messenger RNA expression of estrogen receptor-alpha and -beta in patients with ovarian endometriosis. Fertil Steril 2000;73(6):1219–1225 Stanke DF, Sikes JD, DeYoung DW, Tumbleson ME: Proteins and amino acids in bovine oviducal fluid. J Reprod Fertil 1974;38(2):493–496 McDonald MF, Bellve AR: Influence of oestrogen and progesterone on flow of fluid from the Fallopian tube in the ovariectomized ewe. J Reprod Fertil 1969;20(1):51–61 Briton-Jones C, Lok IH, Yuen PM, Chiu TT, Cheung LP, Haines C: Regulation of human oviductin mRNA expression in vivo. Fertil Steril 2001;75(5):942–946 Murray MK: Biosynthesis and immunocytochemical localization of an estrogen-dependent glycoprotein and associated morphological alterations in the sheep ampulla oviduct. Biol Reprod 1992;47(5):889–902 Kaleczyc J: Effect of estradiol and progesterone on noradrenaline content in nerves of the oviduct, uterus and vagina in ovariectomized pigs. Folia Histochem Cytobiol 1994;32(2):119–126 Goodger AM, Rogers PA: Endometrial endothelial cell proliferation during the menstrual cycle. Hum Reprod 1994;9(3):399–405 Hazzard TM, Molskness TA, Chaffin CL, Stouffer RL: Vascular endothelial growth factor (VEGF) and angiopoietin regulation by gonadotrophin and steroids in macaque granulosa cells during the peri-ovulatory interval. Mol Hum Reprod 1999;5(12):1115–1121 Author information Authors and Affiliations Corresponding author Rights and permissions About this article Cite this article Lam, P.M., Po, L.S., Cheung, L.P. et al. The effect of exogenous estradiol treatment on the mRNA expression of vascular endothelial growth factor and its receptors in cultured human oviduct mucosal cells. J Assist Reprod Genet 22, 251–255 (2005). https://doi.org/10.1007/s10815-005-5148-3 Received: Accepted: Issue date: DOI: https://doi.org/10.1007/s10815-005-5148-3