Modeling Endometriosis in Mice and Pharmacological Activity of the Drug Endoferin

A. V. Ilyichev, M V Belopolskaya, Belopolskaya MV, Pavshintsev VV, V V Pavshintsev, Areshidze DA, D A Areshidze, A. A. Bustios-Guryanova, Bustios-Guryanova AA, M L Lovat, Lovat ML, Mikhaleva LM, L M Mikhaleva
Bulletin of experimental biology and medicine · 2025 · vol. 180(2) , pp. 229–232 · doi:10.1007/s10517-026-06610-3 · PMID:41811408
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⚙ AI-generated summary by gemini-2.5-flash-lite, 2026-08-08 ⓘ

This study developed a murine endometriosis model to assess the drug endoferin, finding its efficacy highest in a short-term, hormone-free protocol and noting that exogenous estradiol interfered with the drug's activity.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-16 · read from full text ⓘ

The study used a murine endometriosis model created by autotransplantation of uterine horn fragments onto the intestinal mesentery across three experimental paradigms, including estradiol-treated, short-term hormone-free, and prolonged hormone-free conditions with delayed drug administration. Well-demarcated endometriotic lesions were consistently observed, and the drug Endoferin showed its highest pharmacological efficacy in the short-term hormone-free protocol. The authors report that exogenous estradiol interfered with the drug’s observable activity, and they developed a rapid in vivo approach for screening candidate therapeutics, with the main caveat being that hormone conditions can markedly alter drug readouts. This paper is centrally about endometriosis — it models endometriosis in mice and tests the pharmacological activity of Endoferin under different estradiol exposure conditions.

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Abstract

A murine model of endometriosis was employed to evaluate the pharmacological efficacy of therapeutic compounds. Endometriosis was modeled by autotransplantation of uterine horn fragments onto the intestinal mesentery in three experimental paradigms: 1) with subcutaneous administration of estradiol; 2) a short-term protocol without estradiol; 3) a prolonged protocol without estradiol and with delayed administration of the test compound. In all animals, well-demarcated, rounded endometriotic lesions (endometriomas) were consistently observed at the implantation sites. Endoferin demonstrated its highest efficacy under the short-term, hormone-free protocol. The administration of exogenous estradiol interfered with the manifestation of the pharmacological activity of the test drug. Thus, a rapid in vivo method for assessing the therapeutic efficacy of candidate drugs was developed.
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A murine model of endometriosis was employed to evaluate the pharmacological efficacy of therapeutic compounds. Endometriosis was modeled by autotransplantation of uterine horn fragments onto the intestinal mesentery in three experimental paradigms: 1) with subcutaneous administration of estradiol; 2) a short-term protocol without estradiol; 3) a prolonged protocol without estradiol and with delayed administration of the test compound. In all animals, well-demarcated, rounded endometriotic lesions (endometriomas) were consistently observed at the implantation sites. Endoferin demonstrated its highest efficacy under the short-term, hormone-free protocol. The administration of exogenous estradiol interfered with the manifestation of the pharmacological activity of the test drug. Thus, a rapid in vivo method for assessing the therapeutic efficacy of candidate drugs was developed. Similar content being viewed by others References Endometriosis. Clinical Guidelines 2024. https://cr.minzdrav.gov.ru/view-cr/259_2 Nishimoto-Kakiuchi A, Sato I, Nakano K, et al. A longacting anti-IL-8 antibody improves inflammation and fibrosis in endometriosis. Sci Transl Med. 2023;15:eabq5858. https://doi.org/10.1126/scitranslmed.abq5858 Adamyan LV, Mikhaleva LM, Serezhenkov VA, et al. Modern view on the experimental creation of endometriosis in animal models (own data). Modern Reproductive Technologies. 2024;30(6):55-60. Russian. https://doi.org/10.17116/repro20243006155 He Y, Liang B, Hung SW, et al. Re-evaluation of mouse models of endometriosis for pathological and immunological research. Front Immunol. 2022;13:986202. https://doi.org/10.3389/fimmu.2022.986202 Zhao Y, Wang Y, Gu P, et al. Transgenic mice applications in the study of endometriosis pathogenesis. Front Cell Dev Biol. 2024;12:1376414. https://doi.org/10.3389/fcell.2024.1376414 Tejada MA, Antunez C, Nunez-Badinez P, et al. Rodent animal models of endometriosis-associated pain: unmet needs and resources available for improving translational research in endometriosis. Int J Mol Sci. 2023;24(3):2422. https://doi.org/10.3390/ijms24032422 Castro J, Maddern J, Grundy L, et al. A mouse model of endometriosis that displays vaginal, colon, cutaneous, and bladder sensory comorbidities. FASEB J. 2021;35(4):e21430. https://doi.org/10.1096/fj.202002441R Zayratyants OV, Andreeva EN, Adamyan LV, et al. Endometriosis: new experience of non-hormonal treatment. Modern Reproductive Technologies. 2018;24(6):113-20. Russian. https://doi.org/10.17116/repro201824061113 Zheng R, Du X, Lei Y. Correlations between endometriosis, lipid profile, and estrogen levels. Medicine (Baltimore). 2023;102(29):e34348. https://doi.org/10.1097/MD.0000000000034348 Huang QM, Dai YD, Lin X, et al. Method for developing mouse model of endometrios is based on estrogenreleasing thermo-sensitive hydrogel system. Zhonghua Yi Xue Za Zhi. 2019;99(23):1819-23. Chinese. https://doi.org/10.3760/cma.j.issn.0376-2491.2019.23.014 Chantalat E, Valera MC, Vaysse C, et al. Estrogen receptors and endometriosis. Int J Mol Sci. 2020;21(8):2815. https://doi.org/10.3390/ijms21082815 Brinca AT, Peiró AM, Evangelio PM, et al. Follicular fluid and blood monitorization of infertility biomarkers in women with endometriosis. Int J Mol Sci. 2024;25(13):7177. https://doi.org/10.3390/ijms25137177 Author information Authors and Affiliations Corresponding author Additional information Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 180, No. 8, pp. 203-207, August, 2025 Rights and permissions Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. About this article Cite this article Ilyichev, A.V., Belopolskaya, M.V., Pavshintsev, V.V. et al. Modeling Endometriosis in Mice and Pharmacological Activity of the Drug Endoferin. Bull Exp Biol Med 180, 229–232 (2025). https://doi.org/10.1007/s10517-026-06610-3 Received: Accepted: Published: Version of record: Issue date: DOI: https://doi.org/10.1007/s10517-026-06610-3

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endometriosis

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Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis

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