{"paper_id":"0dc47614-08f2-4a18-a888-e2c7720ad33c","body_text":"A murine model of endometriosis was employed to evaluate the pharmacological efficacy of therapeutic compounds. Endometriosis was modeled by autotransplantation of uterine horn fragments onto the intestinal mesentery in three experimental paradigms: 1) with subcutaneous administration of estradiol; 2) a short-term protocol without estradiol; 3) a prolonged protocol without estradiol and with delayed administration of the test compound. In all animals, well-demarcated, rounded endometriotic lesions (endometriomas) were consistently observed at the implantation sites. Endoferin demonstrated its highest efficacy under the short-term, hormone-free protocol. The administration of exogenous estradiol interfered with the manifestation of the pharmacological activity of the test drug. Thus, a rapid in vivo method for assessing the therapeutic efficacy of candidate drugs was developed.\nSimilar content being viewed by others\nReferences\nEndometriosis. Clinical Guidelines 2024. https://cr.minzdrav.gov.ru/view-cr/259_2\nNishimoto-Kakiuchi A, Sato I, Nakano K, et al. A longacting anti-IL-8 antibody improves inflammation and fibrosis in endometriosis. Sci Transl Med. 2023;15:eabq5858. https://doi.org/10.1126/scitranslmed.abq5858\nAdamyan LV, Mikhaleva LM, Serezhenkov VA, et al. Modern view on the experimental creation of endometriosis in animal models (own data). Modern Reproductive Technologies. 2024;30(6):55-60. Russian. https://doi.org/10.17116/repro20243006155\nHe Y, Liang B, Hung SW, et al. Re-evaluation of mouse models of endometriosis for pathological and immunological research. Front Immunol. 2022;13:986202. https://doi.org/10.3389/fimmu.2022.986202\nZhao Y, Wang Y, Gu P, et al. Transgenic mice applications in the study of endometriosis pathogenesis. Front Cell Dev Biol. 2024;12:1376414. https://doi.org/10.3389/fcell.2024.1376414\nTejada MA, Antunez C, Nunez-Badinez P, et al. Rodent animal models of endometriosis-associated pain: unmet needs and resources available for improving translational research in endometriosis. Int J Mol Sci. 2023;24(3):2422. https://doi.org/10.3390/ijms24032422\nCastro J, Maddern J, Grundy L, et al. A mouse model of endometriosis that displays vaginal, colon, cutaneous, and bladder sensory comorbidities. FASEB J. 2021;35(4):e21430. https://doi.org/10.1096/fj.202002441R\nZayratyants OV, Andreeva EN, Adamyan LV, et al. Endometriosis: new experience of non-hormonal treatment. Modern Reproductive Technologies. 2018;24(6):113-20. Russian. https://doi.org/10.17116/repro201824061113\nZheng R, Du X, Lei Y. Correlations between endometriosis, lipid profile, and estrogen levels. Medicine (Baltimore). 2023;102(29):e34348. https://doi.org/10.1097/MD.0000000000034348\nHuang QM, Dai YD, Lin X, et al. Method for developing mouse model of endometrios is based on estrogenreleasing thermo-sensitive hydrogel system. Zhonghua Yi Xue Za Zhi. 2019;99(23):1819-23. Chinese. https://doi.org/10.3760/cma.j.issn.0376-2491.2019.23.014\nChantalat E, Valera MC, Vaysse C, et al. Estrogen receptors and endometriosis. Int J Mol Sci. 2020;21(8):2815. https://doi.org/10.3390/ijms21082815\nBrinca AT, Peiró AM, Evangelio PM, et al. Follicular fluid and blood monitorization of infertility biomarkers in women with endometriosis. Int J Mol Sci. 2024;25(13):7177. https://doi.org/10.3390/ijms25137177\nAuthor information\nAuthors and Affiliations\nCorresponding author\nAdditional information\nPublisher's Note\nSpringer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.\nTranslated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 180, No. 8, pp. 203-207, August, 2025\nRights and permissions\nSpringer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.\nAbout this article\nCite this article\nIlyichev, A.V., Belopolskaya, M.V., Pavshintsev, V.V. et al. Modeling Endometriosis in Mice and Pharmacological Activity of the Drug Endoferin. Bull Exp Biol Med 180, 229–232 (2025). https://doi.org/10.1007/s10517-026-06610-3\nReceived:\nAccepted:\nPublished:\nVersion of record:\nIssue date:\nDOI: https://doi.org/10.1007/s10517-026-06610-3","source_license":"public-domain-us","license_restricted":false}