Estrogen Receptor Reduces CYP1A1 Induction in Cultured Human Endometrial Cells

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) exerts its toxic action via the aryl hydrocarbon (Ah) receptor, which induces a battery of xenobiotic-metabolizing enzymes, including the cytochrome P450 isozyme, CYP1A1. TCDD-induced 7-ethoxycoumarin-O-deethylase activity was reduced 75% in cultured human endometrial ECC-1 cells exposed to various concentrations of 17β-estradiol for up to 72 h, with a half-maximal effective concentration (EC50) of 0.9 nm. Reduced enzyme activity was correlated with decreased CYP1A1 mRNA levels, and transcription. Exposure to 17β-estradiol reduced CYP1A1 activity in cells in human cells human the was to and TCDD-induced CYP1A1 mRNA levels, and activity in ECC-1 17β-estradiol to its the of to reduced CYP1A1 transcription. ECC-1 cells with a in CYP1A1 17β-estradiol of 17β-estradiol reduced CYP1A1 the a with and 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) exerts its toxic action via the aryl hydrocarbon (Ah) receptor, which induces a battery of xenobiotic-metabolizing enzymes, including the cytochrome P450 isozyme, CYP1A1. TCDD-induced 7-ethoxycoumarin-O-deethylase activity was reduced 75% in cultured human endometrial ECC-1 cells exposed to various concentrations of 17β-estradiol for up to 72 h, with a half-maximal effective concentration (EC50) of 0.9 nm. Reduced enzyme activity was correlated with decreased CYP1A1 mRNA levels, and transcription. Exposure to 17β-estradiol reduced CYP1A1 activity in cells in human cells human the was to and TCDD-induced CYP1A1 mRNA levels, and activity in ECC-1 17β-estradiol to its the of to reduced CYP1A1 transcription. ECC-1 cells with a in CYP1A1 17β-estradiol of 17β-estradiol reduced CYP1A1 the a with and aryl hydrocarbon cytochrome P450 cytochrome P450 of aryl hydrocarbon cytochrome P450 cytochrome P450 of the of a of in the Exposure of to in toxic and for a in the of of action and to with the a a with and of various including for the of the and to and in and a the receptor, a a in to of which exerts the of and in and endometrial reduced in exposed to a of in exposed to a in the and of a correlated with of with in its in with to the and to including of cultured cells and in a a with to its induces cytochrome P450 the to various and in to 17β-estradiol in to cytochrome P450 activity in was reduced cytochrome aryl hydrocarbon activity in and cultured cells was to in the the of and endometrial to the which ECC-1 cells and TCDD-induced CYP1A1 was the ECC-1 exposed to of CYP1A1 and activity in exposed to of CYP1A1 activity to 17β-estradiol was to of to and of 17β-estradiol of TCDD-induced of a of cytochrome P450 the of of of was to of activity activity for of cytochrome P450 in ECC-1 endometrial cells and to which induces CYP1A1 activity in a and Reduced CYP1A1 17β-estradiol of CYP1A1 activity was in ECC-1 exposed to of and various concentrations of reduced CYP1A1 activity in a for 17β-estradiol of activity was 0.9 which with the for 17β-estradiol in ECC-1 cells CYP1A1 activity in the of 17β-estradiol was reduced 75% with in cells exposed to of 17β-estradiol action was ECC-1 to 17β-estradiol in a in activity the of activity the was of CYP1A1 activity ECC-1 to 17β-estradiol in the of of and was the ECC-1 to a in TCDD-induced activity exposed to the of CYP1A1. exposed to a and exposed to a a in the and the in ECC-1 cells to of the and to the the with the the CYP1A1 the in action was of of the receptor, 17β-estradiol of CYP1A1 and 17β-estradiol to and in ECC-1 exposed to of and and activity was of of TCDD-induced activity in a for and and of action a for in of of CYP1A1 17β-estradiol action and of cytochrome P450 exposed to a of 17β-estradiol in the of various concentrations of for of activity the activity in exposed to was of and was of CYP1A1 activity was in the in of the in CYP1A1 activity was to ECC-1 endometrial the of 17β-estradiol human cells ECC-1 and 17β-estradiol in and a human and a human of which to ECC-1 17β-estradiol reduced TCDD-induced CYP1A1 activity in of CYP1A1 in and cells was the of 17β-estradiol a in the for and to in cells ECC-1 and cells of with and reduced CYP1A1 activity in of ECC-1 human endometrial human and human cells exposed for to with was CYP1A1 activity was in the to the in CYP1A1 mRNA reduced activity CYP1A1 mRNA of CYP1A1 mRNA in ECC-1 cells exposed to and 17β-estradiol cells exposed to concentrations of and 17β-estradiol a of TCDD-induced CYP1A1 mRNA with cells exposed to exposed to CYP1A1 of the reduced CYP1A1 mRNA to of TCDD-induced h, in with the in activity exposed of ECC-1 cells to of and the and action the of CYP1A1 mRNA and CYP1A1 of exerts CYP1A1 mRNA its of ECC-1 with a a of the for the human CYP1A1 the and the the of the cells to activity in the of cells to and 17β-estradiol in a in TCDD-induced activity of the of TCDD-induced of the of ECC-1 cells of cells with a a of the of the CYP1A1 of with of and exposed to the in for and activity was and to the in to the was was 17β-estradiol and the in CYP1A1 mRNA the and ECC-1 exposed to and reduced CYP1A1 of CYP1A1 mRNA of which was for Exposure of ECC-1 to 17β-estradiol reduced a levels, and of enzyme was of ECC-1 to decreased of TCDD-induced CYP1A1. exposed to 17β-estradiol for the of in the of mRNA was and to CYP1A1 and human of of of was to for and 17β-estradiol a to the in reduced of to exposed to to and 17β-estradiol a in ECC-1 exposed to and 17β-estradiol with exposed to of of in of ECC-1 cells exposed to to the with the of the to with reduced to ECC-1 cells exposed to and 17β-estradiol in with for h, which of and to of the exposed to in the of and mRNA the in CYP1A1 and was to a 17β-estradiol the mRNA of to of in ECC-1 exposed to various concentrations of 17β-estradiol for of mRNA was of the cells to the CYP1A1 decreased a of 17β-estradiol the was to was with of of CYP1A1 and and for the of CYP1A1 in the with to the and of the to CYP1A1. of in ECC-1 cells the of ECC-1 cells with the the of human and a the for of the of 17β-estradiol of TCDD-induced of ECC-1 cells with of CYP1A1 transcription. ECC-1 cells with of in a of was with 17β-estradiol 17β-estradiol a for h, and activity was the of in of CYP1A1 activity a of of cultured human endometrial cells exposed to 17β-estradiol reduced CYP1A1 mRNA levels, and activity with exposed to was in with the of CYP1A1 and was action was and was which Exposure of ECC-1 to action of CYP1A1 of CYP1A1 activity was cells and ECC-1 endometrial and of receptor, decreased CYP1A1 activity in the of CYP1A1 activity in human cells human cells and of the and and was in human cells and cells to the of the to action and in the the to a the 17β-estradiol to its the of 17β-estradiol of of was to CYP1A1 mRNA levels, which to of the CYP1A1 its transcription. of the and the for and of the and and for the for of the of the to the of CYP1A1 the of CYP1A1 activity of was in ECC-1 of action CYP1A1 of the of which in and with and transcription. with the to 17β-estradiol the receptor, of to CYP1A1 and the to in of CYP1A1 to the action of with and of in the of and to and the of to the of of for the to CYP1A1 the the to transcription. of of with of in ECC-1 cells to CYP1A1 was ECC-1 to 17β-estradiol was to the of endometrial of CYP1A1 17β-estradiol for the the endometrial of 17β-estradiol the a of activity in the to a of of the and the to in in the its and with for the receptor, which of and in various of to in to of in CYP1A1 in human exposed to was of 17β-estradiol the of CYP1A1 activity a 17β-estradiol and a to the a receptor, of CYP1A1 to in action of of in cells of 17β-estradiol and and action and activity in human endometrial the a of and for and aryl hydrocarbon cytochrome P450 cytochrome P450 of aryl hydrocarbon cytochrome P450 cytochrome P450 of the of a of in the Exposure of to in toxic and for a in the of of action and to with the a a with and of various including for the of the and to and in and a the receptor, a a in to of which exerts the of and in and endometrial reduced in exposed to a of in exposed to a in the and of a correlated with of with in its in with to the and to including of cultured cells and in a a with to its induces cytochrome P450 the to various and in to 17β-estradiol in to cytochrome P450 activity in was reduced cytochrome aryl hydrocarbon activity in and cultured cells was to in the the of and endometrial to the which ECC-1 cells and TCDD-induced CYP1A1 was the ECC-1 exposed to of CYP1A1 and activity in exposed to of CYP1A1 activity to 17β-estradiol was to of to and of 17β-estradiol of TCDD-induced of a of cytochrome P450 the of of of was to of activity activity for of cytochrome P450 in ECC-1 endometrial cells and to which induces CYP1A1 activity in a and Reduced CYP1A1 17β-estradiol of CYP1A1 activity was in ECC-1 exposed to of and various concentrations of reduced CYP1A1 activity in a for 17β-estradiol of activity was 0.9 which with the for 17β-estradiol in ECC-1 cells CYP1A1 activity in the of 17β-estradiol was reduced 75% with in cells exposed to of 17β-estradiol action was ECC-1 to 17β-estradiol in a in activity the of activity the was of CYP1A1 activity ECC-1 to 17β-estradiol in the of of and was the ECC-1 to a in TCDD-induced activity exposed to the of CYP1A1. exposed to a and exposed to a a in the and the in ECC-1 cells to of the and to the the with the the CYP1A1 the in action was of of the receptor, 17β-estradiol of CYP1A1 and 17β-estradiol to and in ECC-1 exposed to of and and activity was of of TCDD-induced activity in a for and and of action a for in of of CYP1A1 of the in CYP1A1 activity was to ECC-1 endometrial the of 17β-estradiol human cells ECC-1 and 17β-estradiol in and a human and a human of which to ECC-1 17β-estradiol reduced TCDD-induced CYP1A1 activity in of CYP1A1 in and cells was the of 17β-estradiol a in the for and to in cells ECC-1 and cells of with and reduced CYP1A1 activity in of ECC-1 human endometrial human and human cells exposed for to with was CYP1A1 activity was in the to the in CYP1A1 mRNA reduced activity CYP1A1 mRNA of CYP1A1 mRNA in ECC-1 cells exposed to and 17β-estradiol cells exposed to concentrations of and 17β-estradiol a of TCDD-induced CYP1A1 mRNA with cells exposed to exposed to CYP1A1 of the reduced CYP1A1 mRNA to of TCDD-induced h, in with the in activity exposed of ECC-1 cells to of and the and action the of CYP1A1 mRNA and CYP1A1 of exerts CYP1A1 mRNA its of ECC-1 with a a of the for the human CYP1A1 the and the the of the cells to activity in the of cells to and 17β-estradiol in a in TCDD-induced activity of the of TCDD-induced of the of ECC-1 cells of cells with a a of the of the CYP1A1 of with of and exposed to the in for and activity was and to the in to the was was 17β-estradiol and the in CYP1A1 mRNA the and ECC-1 exposed to and reduced CYP1A1 of CYP1A1 mRNA of which was for Exposure of ECC-1 to 17β-estradiol reduced a levels, and of enzyme was of ECC-1 to decreased of TCDD-induced CYP1A1. exposed to 17β-estradiol for the of in the of mRNA was and to CYP1A1 and human of of of was to for and 17β-estradiol a to the in reduced of to exposed to to and 17β-estradiol a in ECC-1 exposed to and 17β-estradiol with exposed to of of in of ECC-1 cells exposed to to the with the of the to with reduced to ECC-1 cells exposed to and 17β-estradiol in with for h, which of and to of the exposed to in the of and mRNA the in CYP1A1 and was to a 17β-estradiol the mRNA of to of in ECC-1 exposed to various concentrations of 17β-estradiol for of mRNA was of the cells to the CYP1A1 decreased a of 17β-estradiol the was to was with of of CYP1A1 and and for the of CYP1A1 in the with to the and of the to CYP1A1. of in ECC-1 cells the of ECC-1 cells with the the of human and a the for of the of 17β-estradiol of TCDD-induced of ECC-1 cells with of CYP1A1 transcription. ECC-1 cells with of in a of was with 17β-estradiol 17β-estradiol a for h, and activity was the of in of cytochrome P450 the of of of was to of activity activity for of cytochrome P450 in ECC-1 endometrial cells and to which induces CYP1A1 activity in a and Reduced CYP1A1 17β-estradiol of CYP1A1 activity was in ECC-1 exposed to of and various concentrations of reduced CYP1A1 activity in a for 17β-estradiol of activity was 0.9 which with the for 17β-estradiol in ECC-1 cells CYP1A1 activity in the of 17β-estradiol was reduced 75% with in cells exposed to of 17β-estradiol action was ECC-1 to 17β-estradiol in a in activity the of activity the was of CYP1A1 activity ECC-1 to 17β-estradiol in the of of and was the ECC-1 to a in TCDD-induced activity exposed to the of CYP1A1. exposed to a and exposed to a a in the and the in ECC-1 cells to of the and to the the with the the CYP1A1 the in action was of of the receptor, 17β-estradiol of CYP1A1 and 17β-estradiol to and in ECC-1 exposed to of and and activity was of of TCDD-induced activity in a for and and of action a for in of of CYP1A1 of the in CYP1A1 activity was to ECC-1 endometrial the of 17β-estradiol human cells ECC-1 and 17β-estradiol in and a human and a human of which to ECC-1 17β-estradiol reduced TCDD-induced CYP1A1 activity in of CYP1A1 in and cells was the of 17β-estradiol a in the for and to in cells ECC-1 and cells of with and CYP1A1 mRNA reduced activity CYP1A1 mRNA of CYP1A1 mRNA in ECC-1 cells exposed to and 17β-estradiol cells exposed to concentrations of and 17β-estradiol a of TCDD-induced CYP1A1 mRNA with cells exposed to exposed to CYP1A1 of the reduced CYP1A1 mRNA to of TCDD-induced h, in with the in activity exposed of ECC-1 cells to of and the and action the of CYP1A1 mRNA and CYP1A1 of exerts CYP1A1 mRNA its of ECC-1 with a a of the for the human CYP1A1 the and the the of the cells to activity in the of cells to and 17β-estradiol in a in TCDD-induced activity of the of TCDD-induced of the of ECC-1 cells of cells with a a of the of the CYP1A1 of with of and exposed to the in for and activity was and to the in to the was was 17β-estradiol and the in CYP1A1 mRNA the and ECC-1 exposed to and reduced CYP1A1 of CYP1A1 mRNA of which was for Exposure of ECC-1 to 17β-estradiol reduced a levels, and of enzyme was of ECC-1 to decreased of TCDD-induced CYP1A1. exposed to 17β-estradiol for the of in the of mRNA was and to CYP1A1 and human of of of was to for and 17β-estradiol a to the in reduced of to exposed to to and 17β-estradiol a in ECC-1 exposed to and 17β-estradiol with exposed to of of in of ECC-1 cells exposed to to the with the of the to with reduced to ECC-1 cells exposed to and 17β-estradiol in with for h, which of and to of the exposed to in the of and mRNA the in CYP1A1 and was to a 17β-estradiol the mRNA of to of in ECC-1 exposed to various concentrations of 17β-estradiol for of mRNA was of the cells to the CYP1A1 decreased a of 17β-estradiol the was to was with of of CYP1A1 and and for the of CYP1A1 in the with to the and of the to CYP1A1. of in ECC-1 cells the of ECC-1 cells with the the of human and a the for of the of 17β-estradiol of TCDD-induced of ECC-1 cells with of CYP1A1 transcription. ECC-1 cells with of in a of was with 17β-estradiol 17β-estradiol a for h, and activity was the of in Reduced CYP1A1 17β-estradiol of CYP1A1 activity was in ECC-1 exposed to of and various concentrations of reduced CYP1A1 activity in a for 17β-estradiol of activity was 0.9 which with the for 17β-estradiol in ECC-1 cells CYP1A1 activity in the of 17β-estradiol was reduced 75% with in cells exposed to of 17β-estradiol action was ECC-1 to 17β-estradiol in a in activity the of activity the was of CYP1A1 activity ECC-1 to 17β-estradiol in the of of and was the ECC-1 to a in TCDD-induced activity exposed to the of CYP1A1. exposed to a and exposed to a a in the and the in ECC-1 cells to of the and to the the with the the CYP1A1 the in action was of of the receptor, 17β-estradiol of CYP1A1 and 17β-estradiol to and in ECC-1 exposed to of and and activity was of of TCDD-induced activity in a for and and of action a for in of of CYP1A1 of the in CYP1A1 activity was to ECC-1 endometrial the of 17β-estradiol human cells ECC-1 and 17β-estradiol in and a human and a human of which to ECC-1 17β-estradiol reduced TCDD-induced CYP1A1 activity in of CYP1A1 in and cells was the of 17β-estradiol a in the for and to in cells ECC-1 and cells of with and CYP1A1 mRNA reduced activity CYP1A1 mRNA of CYP1A1 mRNA in ECC-1 cells exposed to and 17β-estradiol cells exposed to concentrations of and 17β-estradiol a of TCDD-induced CYP1A1 mRNA with cells exposed to exposed to CYP1A1 of the reduced CYP1A1 mRNA to of TCDD-induced h, in with the in activity exposed of ECC-1 cells to of and the and action the of CYP1A1 mRNA and CYP1A1 of exerts CYP1A1 mRNA its of ECC-1 with a a of the for the human CYP1A1 the and the the of the cells to activity in the of cells to and 17β-estradiol in a in TCDD-induced activity of the of TCDD-induced of the the in CYP1A1 mRNA the and ECC-1 exposed to and reduced CYP1A1 of CYP1A1 mRNA of which was for Exposure of ECC-1 to 17β-estradiol reduced a levels, and of enzyme was of ECC-1 to decreased of TCDD-induced CYP1A1. exposed to 17β-estradiol for the of in the of mRNA was and to CYP1A1 and human of of of was to for and 17β-estradiol a to the in reduced of to exposed to to and 17β-estradiol a in ECC-1 exposed to and 17β-estradiol with exposed to of of in of ECC-1 cells exposed to to the with the of the to with reduced to ECC-1 cells exposed to and 17β-estradiol in with for h, which of and to of the exposed to in the of and mRNA the in CYP1A1 and was to a 17β-estradiol the mRNA of to of in ECC-1 exposed to various concentrations of 17β-estradiol for of mRNA was of the cells to the CYP1A1 decreased a of 17β-estradiol the was to was with of of CYP1A1 and and for the of CYP1A1 in the with to the and of the to CYP1A1. of in ECC-1 cells the of ECC-1 cells with the the of human and a the for of the of 17β-estradiol of TCDD-induced of ECC-1 cells with of CYP1A1 transcription. ECC-1 cells with of in a of was with 17β-estradiol 17β-estradiol a for h, and activity was the of in Reduced CYP1A1 17β-estradiol of CYP1A1 activity was in ECC-1 exposed to of and various concentrations of reduced CYP1A1 activity in a for 17β-estradiol of activity was 0.9 which with the for 17β-estradiol in ECC-1 cells CYP1A1 activity in the of 17β-estradiol was reduced 75% with in cells exposed to of 17β-estradiol action was ECC-1 to 17β-estradiol in a in activity the of activity the was of CYP1A1 activity ECC-1 to 17β-estradiol in the of of and was the 17β-estradiol of CYP1A1 activity was in ECC-1 exposed to of and various concentrations of reduced CYP1A1 activity in a for 17β-estradiol of activity was 0.9 which with the for 17β-estradiol in ECC-1 cells CYP1A1 activity in the of 17β-estradiol was reduced 75% with in cells exposed to of 17β-estradiol action was ECC-1 to 17β-estradiol in a in activity the of activity the was of CYP1A1 activity ECC-1 to 17β-estradiol in the of of and was the ECC-1 to a in TCDD-induced activity exposed to the of CYP1A1. exposed to a and exposed to a a in the and the in ECC-1 cells to of the and to the the with the the CYP1A1 the in action was ECC-1 to a in TCDD-induced activity exposed to the of CYP1A1. exposed to a and exposed to a a in the and the in ECC-1 cells to of the and to the the with the the CYP1A1 the in action was of of the receptor, 17β-estradiol of CYP1A1 and 17β-estradiol to and in ECC-1 exposed to of and and activity was of of TCDD-induced activity in a for and and of action a for in of of CYP1A1 of the receptor, 17β-estradiol of CYP1A1 and 17β-estradiol to and in ECC-1 exposed to of and and activity was of of TCDD-induced activity in a for and and of action a for in of of CYP1A1 of the in CYP1A1 activity was to ECC-1 endometrial the of 17β-estradiol human cells ECC-1 and 17β-estradiol in and a human and a human of which to ECC-1 17β-estradiol reduced TCDD-induced CYP1A1 activity in of CYP1A1 in and cells was the of 17β-estradiol a in the for and to in cells ECC-1 and cells of with and the in CYP1A1 activity was to ECC-1 endometrial the of 17β-estradiol human cells ECC-1 and 17β-estradiol in and a human and a human of which to ECC-1 17β-estradiol reduced TCDD-induced CYP1A1 activity in of CYP1A1 in and cells was the of 17β-estradiol a in the for and to in cells ECC-1 and cells of with and CYP1A1 mRNA reduced activity CYP1A1 mRNA of CYP1A1 mRNA in ECC-1 cells exposed to and 17β-estradiol cells exposed to concentrations of and 17β-estradiol a of TCDD-induced CYP1A1 mRNA with cells exposed to exposed to CYP1A1 of the reduced CYP1A1 mRNA to of TCDD-induced h, in with the in activity exposed of ECC-1 cells to of and the and action the of CYP1A1 mRNA and CYP1A1 reduced activity CYP1A1 mRNA of CYP1A1 mRNA in ECC-1 cells exposed to and 17β-estradiol cells exposed to concentrations of and 17β-estradiol a of TCDD-induced CYP1A1 mRNA with cells exposed to exposed to CYP1A1 of the reduced CYP1A1 mRNA to of TCDD-induced h, in with the in activity exposed of ECC-1 cells to of and the and action the of CYP1A1 mRNA and CYP1A1 of exerts CYP1A1 mRNA its of ECC-1 with a a of the for the human CYP1A1 the and the the of the cells to activity in the of cells to and 17β-estradiol in a in TCDD-induced activity of the of TCDD-induced of the the in CYP1A1 mRNA the and ECC-1 exposed to and reduced CYP1A1 of CYP1A1 mRNA of which was for Exposure of ECC-1 to 17β-estradiol reduced a levels, and of enzyme was of ECC-1 to exerts CYP1A1 mRNA its of ECC-1 with a a of the for the human CYP1A1 the and the the of the cells to activity in the of cells to and 17β-estradiol in a in TCDD-induced activity of the of TCDD-induced of the the in CYP1A1 mRNA the and ECC-1 exposed to and reduced CYP1A1 of CYP1A1 mRNA of which was for Exposure of ECC-1 to 17β-estradiol reduced a levels, and of enzyme was of ECC-1 to a to the in reduced of to exposed to to and 17β-estradiol a in ECC-1 exposed to and 17β-estradiol with exposed to of of in of ECC-1 cells exposed to to the with the of the to with a to the in reduced of to exposed to to and 17β-estradiol a in ECC-1 exposed to and 17β-estradiol with exposed to of of in of ECC-1 cells exposed to to the with the of the to with mRNA the in CYP1A1 and was to a 17β-estradiol the mRNA of to of in ECC-1 exposed to various concentrations of 17β-estradiol for of mRNA was of the cells to the CYP1A1 decreased a of 17β-estradiol the was to was with the in CYP1A1 and was to a 17β-estradiol the mRNA of to of in ECC-1 exposed to various concentrations of 17β-estradiol for of mRNA was of the cells to the CYP1A1 decreased a of 17β-estradiol the was to was with of of CYP1A1 and and for the of CYP1A1 in the with to the and of the to CYP1A1. of in ECC-1 cells the of ECC-1 cells with the the of human and a the for of the of 17β-estradiol of TCDD-induced of CYP1A1 and and for the of CYP1A1 in the with to the and of the to CYP1A1. of in ECC-1 cells the of ECC-1 cells with the the of human and a the for of the of 17β-estradiol of TCDD-induced of CYP1A1 activity a of of cultured human endometrial cells exposed to 17β-estradiol reduced CYP1A1 mRNA levels, and activity with exposed to was in with the of CYP1A1 and was action was and was which Exposure of ECC-1 to action of CYP1A1 of CYP1A1 activity was cells and ECC-1 endometrial and of receptor, decreased CYP1A1 activity in the of CYP1A1 activity in human cells human cells and of the and and was in human cells and cells to the of the to action and in the the to a the 17β-estradiol to its the of 17β-estradiol of of was to CYP1A1 mRNA levels, which to of the CYP1A1 its transcription. of the and the for and of the and and for the for of the of the to the of CYP1A1 the of CYP1A1 activity of was in ECC-1 of action CYP1A1 of the of which in and with and transcription. with the to 17β-estradiol the receptor, of to CYP1A1 and the to in of CYP1A1 to the action of with and of in the of and to and the of to the of of for the to CYP1A1 the the to transcription. of of with of in ECC-1 cells to CYP1A1 was ECC-1 to 17β-estradiol was to the of endometrial of CYP1A1 17β-estradiol for the the endometrial of 17β-estradiol the a of activity in the to a of of the and the to in in the its and with for the receptor, which of and in various of to in to of in CYP1A1 in human exposed to was of 17β-estradiol the of CYP1A1 activity a 17β-estradiol and a to the a receptor, of CYP1A1 to in action of of in cells of 17β-estradiol and and action and activity in human endometrial the a of and for and of CYP1A1 activity a of of cultured human endometrial cells exposed to 17β-estradiol reduced CYP1A1 mRNA levels, and activity with exposed to was in with the of CYP1A1 and was action was and was which Exposure of ECC-1 to action of CYP1A1 of CYP1A1 activity was cells and ECC-1 endometrial and of receptor, decreased CYP1A1 activity in the of CYP1A1 activity in human cells human cells and of the and and was in human cells and cells to the of the to action and in the the to a the 17β-estradiol to its the of 17β-estradiol of of was to CYP1A1 mRNA levels, which to of the CYP1A1 its transcription. of the and the for and of the and and for the for of the of the to the of CYP1A1 the of CYP1A1 activity of was in ECC-1 of action CYP1A1 of the of which in and with and transcription. with the to 17β-estradiol the receptor, of to CYP1A1 and the to in of CYP1A1 to the action of with and of in the of and to and the of to the of of for the to CYP1A1 the the to transcription. of of with of in ECC-1 cells to CYP1A1 was ECC-1 to 17β-estradiol was to the of endometrial of CYP1A1 17β-estradiol for the the endometrial of 17β-estradiol the a of activity in the to a of of the and the to in in the its and with for the receptor, which of and in various of to in to of in CYP1A1 in human exposed to was of 17β-estradiol the of CYP1A1 activity a 17β-estradiol and a to the a receptor, of CYP1A1 to in action of of in cells of 17β-estradiol and and action and activity in human endometrial the a of and for and for in and for of for in the and for