Sperm retrieval, fertilization, and pregnancy outcome in repeated testicular sperm aspiration.

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Repeated testicular sperm aspiration up to six times yielded sufficient motile sperm for ICSI and maintained fertilization and pregnancy rates without complications.

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Abstract

Purpose: To report the outcome of sperm retrieval and results after ICSI in up to six repeated testicular sperm aspiration procedures.

Methods

Twenty-two men with obstructive and thirty-four men with nonobstructive azoospermia underwent 50 and 91 needle aspirations, respectively. Sufficiency of spermatozoa for ICSI and cryopreservation, fertilization rate, and pregnancy outcome was analyzed retrospectively.

Results

No major differences were found in sperm recovery or pregnancy outcome in the repeated cycles. Testicular aspirate containing motile spermatozoa with maintained fertilizing capacity was obtained in up to six repeated procedures in the nonobstructive group. No postoperative complications were reported for any of the participants.

Conclusions

Testicular sperm aspiration is a simple and effective method of sperm retrieval, which can be performed from the same testis up to several times with good recovery of motile spermatozoa for ICSI and maintaining high fertilization and pregnancy rates, in men with both obstructive and nonobstructive azoospermia. Similar content being viewed by others

References

Temple-Smith PD, Southwick GJ, Yates CA, Trounson AO, de Kretser DM: Human pregnancy by in vitro fertilization (IVF) using sperm aspirated from the epididymis. J In Vitro Fert Embryo Transf 1985;2:119-122 Palermo G, Joris H, Devroey P, Van Steirteghem AC: Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet 1992;340:17-18 Silber SJ, Devroey P, Tournaye H, Van Steirteghem AC: Fertilizing capacity of epididymal and testicular sperm using intracytoplasmic sperm injection (ICSI). Reprod Fertil Dev 1995;7:281-292 Mansour RT, Kamal A, Fahmy I, Tawab N, Serour GI, Aboulghar MA: Intracytoplasmic sperm injection in obstructive and non-obstructive azoospermia. Hum Reprod 1997;12:1974-1979 Kahraman S, Ozgur S, Alatas C, Aksoy S, Tasdemir M, Nuhoglu A, Tasdemir I, Balaban B, Biberoglu K, Schoysman R, Nijs M, Vanderzwalmen P: Fertility with testicular sperm extraction and intracytoplasmic sperm injection in non-obstructive azoospermic men. Hum Reprod 1996;11:756-760 Tournaye H, Devroey P, Liu J, Nagy Z, Lissens W, Van Steirteghem A: Microsurgical epididymal sperm aspiration and intracytoplasmic sperm injection: A new effective approach to infertility as a result of congenital bilateral absence of the vas deferens. Fertil Steril 1994;61:1045-1051 Tucker MJ, Morton PC, Witt MA, Wright G: Intracytoplasmic injection of testicular and epididymal spermatozoa for treatment of obstructive azoospermia. Hum Reprod 1995;10:486-489 Craft I, Shrivastav P: Treatment of male infertility [letter; comment]. Lancet 1994;344:191-192 Craft I, Tsirigotis M: Simplified recovery, preparation and cryopreservation of testicular spermatozoa. Hum Reprod 1995;10:1623-1626 Westlander G, Hamberger L, Hanson C, Lundin K, Nilsson L, Soderlund B, Werner C, Bergh C: Diagnostic epididymal and testicular sperm recovery and genetic aspects in azoospermic men. Hum Reprod 1999;14:118-122 Friedler S, Raziel A, Strassburger D, Soffer Y, Komarovsky D, Ron-El R: Testicular sperm retrieval by percutaneous fine needle sperm aspiration compared with testicular sperm extraction by open biopsy in men with non-obstructive azoospermia. Hum Reprod 1997;12:1488-1493 Tournaye H, Verheyen G, Nagy P, Ubaldi F, Goossens A, Silber S, Van Steirteghem AC, Devroey P: Are there any predictive factors for successful testicular sperm recovery in azoospermic patients? Hum Reprod 1997;12:80-86 Schlegel PN, Su LM: Physiological consequences of testicular sperm extraction. Hum Reprod 1997;12:1688-1692 Ron-El R, Strauss S, Friedler S, Strassburger D, Komarovsky D, Raziel A: Serial sonography and colour flow Doppler imaging following testicular and epididymal sperm extraction. Hum Reprod 1998;13:3390-3393 Rosenlund B, Westlander G, Wood M, Lundin K, Reismer E, Hillensjo T: Sperm retrieval and fertilization in repeated percutaneous epididymal sperm aspiration. Hum Reprod 1998;13:2805-2807 Hamberger L, Sjogren A, Lundin K, Soderlund B, Nilsson L, Bergh C, Wennerholm UB, Wikland M, Svalander P, Jakobsson AH, Forsberg AS: Microfertilization techniques-the Swedish experience. Reprod Fertil Dev 1995;7:263-267 Tournaye H, Liu J, Nagy PZ, Camus M, Goossens A, Silber S, Van Steirteghem AC, Devroey P: Correlation between testicular histology and outcome after intracytoplasmic sperm injection using testicular spermatozoa [see comments]. Hum Reprod 1996;11:127-132 Lewin A, Reubinoff B, Porat-Katz A, Weiss D, Eisenberg V, Arbel R, Bar-el H, Safran A: Testicular fine needle aspiration: The alternative method for sperm retrieval in non-obstructive azoospermia. Hum Reprod 1999;14:1785-1790 Rosenlund B, Kvist U, Ploen L, Rozell BL, Sjoblom P, Hillensjo T: A comparison between open and percutaneous needle biopsies in men with azoospermia. Hum Reprod 1998;13:1266-1271 Ezeh UI, Moore HD, Cooke ID: A prospective study of multiple needle biopsies versus a single open biopsy for testicular sperm extraction in men with non-obstructive azoospermia. Hum Reprod 1998;13:3075-3080 Schlegel PN: Testicular sperm extraction: Microdissection improves sperm yield with minimal tissue excision. Hum Reprod 1999;14:131-135 Harrington TG, Schauer D, Gilbert BR: Percutaneous testis biopsy: An alternative to open testicular biopsy in the evaluation of the subfertile man. J Urol 1996;156:1647-1651 Gorgy A, Meniru GI, Naumann N, Beski S, Bates S, Craft IL: The efficacy of local anaesthesia for percutaneous epididymal sperm aspiration and testicular sperm aspiration. Hum Reprod 1998;13:646-650 Tuuri T, Moilanen J, Kaukoranta S, Makinen S, Kotola S, Hovatta O: Testicular biopty gun needle biopsy in collecting spermatozoa for intracytoplasmic injection, cryopreservation and histology. Hum Reprod 1999;14:1274-1278 Tournaye H, Clasen K, Aytoz A, Nagy Z, Van Steirteghem A, Devroey P: Fine needle aspiration versus open biopsy for testicular sperm recovery: A controlled study in azoospermic patients with normal spermatogenesis. HumReprod 1998;13:901-904 Turek PJ, Cha I, Ljung BM: Systematic fine-needle aspiration of the testis: Correlation to biopsy and results of organ “mapping” for mature sperm in azoospermic men. Urology 1997;49:743-748 Silber SJ, Nagy Z, Devroey P, Tournaye H, Van Steirteghem AC: Distribution of spermatogenesis in the testicles of azoospermic men: The presence or absence of spermatids in the testes of men with germinal failure. Hum Reprod 1997;12:2422-2428 Hauser R, Botchan A, Amit A, Ben Yosef D, Gamzu R, Paz G, Lessing JB, Yogev L, Yavetz H: Multiple testicular sampling in non-obstructive azoospermia-is it necessary? Hum Reprod 1998;13:3081-3085 Author information Authors and Affiliations Rights and permissions About this article Cite this article Westlander, G., Rosenlund, B., Söderlund, B. et al. ANDROLOGY: Sperm Retrieval, Fertilization, and Pregnancy Outcome in Repeated Testicular Sperm Aspiration. J Assist Reprod Genet 18, 171–177 (2001). https://doi.org/10.1023/A:1009459920286 Issue date: DOI: https://doi.org/10.1023/A:1009459920286

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