F&S science

OBJECTIVE: To assess the prevalence of proliferative endometrial disorders in women with histologically confirmed adenomyosis and to evaluate the independent association between adenomyosis and each lesion type. DESIGN: Retrospective cohor…

OBJECTIVE: To investigate estrogen receptor gene 1 (ESR1) and estrogen-driven transcription in human endometrial stromal cells. DESIGN: RNA sequencing (RNA-seq) and Cleavage Under Targets and Release Using Nuclease (Cut&Run) were performed…

OBJECTIVE: To evaluate the effects of letrozole alone and in combination with a gonadotropin-releasing hormone agonist on inflammatory cytokines, folliculogenesis, and hormonal profiles in a rat model of surgically induced peritoneal endome…

OBJECTIVE: To characterize the immune cell and hormonal profile of menstrual effluent compared with paired peripheral blood. DESIGN: This descriptive, cross-sectional study collected menstrual effluent and processed it to isolate cellular …

OBJECTIVE: To investigate if a positive result on ReceptivaDx for evaluation of B-cell lymphoma 6 (BCL6), a proposed marker of progesterone resistance associated with impaired uterine receptivity, correlates with a suboptimal profile of rec…

OBJECTIVE: To study how compressive forces influence fibroid and myometrial cells. Our work aimed to identify proteins and signaling pathways that are altered in fibroids in response to compressive forces. DESIGN: Laboratory-based. SUBJEC…

OBJECTIVE: To characterize the expression of adhesion G protein-coupled receptors (ADGR) in the human endometrium and early mouse pregnancy. DESIGN: An in silico analysis was performed using a retrospective data set comprised endometrial s…

OBJECTIVE: To determine if microRNAs that are altered in the circulation of women with endometriosis affect metabolic gene expression in hepatic cells. DESIGN: In vitro study. SUBJECTS: Deidentified tissue from women with endometriosis. …

OBJECTIVE: Magnetic resonance elastography (MRE), a novel imaging technique that allows in vivo measurement of tissue mechanical properties, was used to test the prediction that the stiffness of the uterus may be increased due to fibrotic c…

OBJECTIVE: To study the effect of laparoscopic ovarian drilling (LOD) vs. step-up gonadotropin therapy on follicular response in infertile anovulatory polycystic ovary syndrome (PCOS) women resistant to sequential letrozole + human menopaus…

OBJECTIVE: To evaluate the effects of a P2X4 receptor (P2X4R)-specific antagonist on murine endometriotic-like lesions and human endometriotic stromal cells. DESIGN: Experimental study using an in vivo mouse endometriosis model and in vitr…

OBJECTIVE: To identify key genes and potential drug targets for ovarian-related diseases through genome-wide Mendelian randomization (MR) and colocalization analyses. DESIGN: We conducted a comprehensive two-sample MR analysis to estimate …

OBJECTIVE: To investigate enhancer-mediated regulation of progesterone receptor (PGR) isoforms, PGR-A and PGR-B, in human endometrial stromal cells, and to determine how isoform modulation shapes the progesterone-responsive transcriptome an…

OBJECTIVE: To use murine avatars for studying human endometriotic lesion response to 2 different hormonal regimens to determine whether progesterone receptor (PR) can prospectively predict response to progestin-based therapy. Endometriosis …

OBJECTIVE: To investigate whether endometrial receptivity is affected in patients with endometriosis using podocalyxin (PCX) as a functional biomarker and to study how endometriotic lesions display PCX and the potential pathological implica…

OBJECTIVE: Stearoyl-CoA desaturase (SCD1) is an enzyme that catalyzes the conversion of saturated delta-9 fatty acids to monounsaturated fatty acids. SCD1 is highly expressed in various cancers and facilitates cancer cell survival, tumor gr…

OBJECTIVE: To compare the transcriptomes induced by estrogens used in combined oral contraceptives (COCs): estetrol (E4), ethinylestradiol (EE), and estradiol (E2) in human primary endometriotic stromal cells. DESIGN: Endometriotic stromal…

OBJECTIVE: To study the role of the mesothelial cells in early endometriosis lesion formation by assessing in vitro cell-to-cell communication and invasion of endometrial cells across a mesothelial cell monolayer, with both cell types deriv…

OBJECTIVE: To evaluate the impact of coenzyme Q-10 (CoQ-10) on the dysregulated synthesis of extracellular matrix proteins mediated by transforming growth factor beta 3 (TGF-β3) in uterine leiomyomas. DESIGN: Laboratory study. SETTING: Un…

OBJECTIVE: To study the effect of adenomyosis on the localized expression of the GATA binding proteins 2 and 6 (GATA2 and GATA6) zinc-finger transcription factors that are involved in proliferation of hematopoietic and endocrine cell lineag…

OBJECTIVE: To study the role of PGE2 in regulating plasminogen activator inhibitor-1 (PAI-1) and tissue plasminogen activator (tPA) in human primary endometrial endothelial cells (HEECs) from women with normal menstrual bleeding (NMB) and h…

Enhancing the efficacy of three-dimensional endometrial assembloid models is pivotal for deepening our comprehension of endometrial physiology. We investigated the optimal ratio of organoids to stromal cells in these models, utilizing human…

OBJECTIVE: To identify cytokines or extracellular matrix components that contribute to adhesion to, and invasion of, the peritoneum, proximal to lesions in the early phase of endometriosis. DESIGN: Laboratory-based study. SETTING: Univers…

Recognizing the limited information, vague diagnostic criterion, and difficult clinical challenges posed by adenomyosis, the Society of Endometriosis and Uterine Disorders organized an expert symposium to present the current understanding o…