Causes, consequences, and therapy of tumors acidosis.

Biochemically, all catabolism is oxidative, resulting in the conversion of fats (hydrocarbons), and carbohydrates (alcohols) into carbonic or keto acids. Fermentation results in production of non-oxidized acids, such as lactic acid, which can be metabolized oxidatively by other organs (liver, kidney) or nearby cells within tumors. Maintenance of systemic and tissue pH values involves a complex system that includes both passive and active buffering. Passive elements include mobile buffers (bicarbonate, phosphate), alkaline ions (Na + , K + ), and immobile buffers (proteins, nucleic acids). Active elements include release of the volatile acid CO 2 in the lungs and the base HCO 3 − or sulfuric acid (from cysteine and methionine) by the kidneys. Urea is excreted by the kidneys and, as it is uncharged at neutral pH, its formation results in the net generation of one H + equivalent and is thus also acidifying. Metabolic acid loads occur intracellularly, and cells have evolved robust and redundant mechanisms to export H + and maintain intracellular pH within strict bounds ( Figure 1 ). Active (ATP-requiring) H + equivalent transporters include: Vacuolar-type H + ATPases that are normally found in lysosomes but can be expressed in the plasma membrane 1 , 2 ; and Na + driven H + extrusion which can be either direct (Na-H exchange, or NHE); or indirect via Na-bicarbonate co-transport, NBC 3 – 5 . Notably, the bicarbonate is dehydrated intracellularly via carbonic anhydrases (usually CA2) into CO 2 (consuming a H + ), which leaves the cell to be re-hydrated extracellularly (producing a H + ) via membrane bound, exofacial carbonic anhydrases (CA4, CA9, or CA12) 6 . Of these, CA9 is active at very low pH values 7 and is considered a “pH-stat” responsible for acidifying the extracellular microenvironment 8 . CA9 has long been known to be a negative prognostic indicator in breast and other cancers 9 . Anion exchanger 2 (AE2) participates in the exchange of Cl − with HCO 3 − , regulating acid-base balance in the intracellular space and micro-environments surrounding cells. A recent study has reported the prognostic value of AE2 expression in esophageal squamous cell carcinoma (ESCC) 10 . AE2 is strongly expressed in ESCC cells, and is coupled to matrix metalloproteinases, migration, and invasion. Although systemic pH is higly regulated, chronic altertions in tissue and systemic pH are associated with many diseases including cancer, diabetes, epilepsy and MELAS. Measurement of intra- and extracellular pH, and its correlations with disease, is an extremely active area of research, because there is a compelling need for robust, accurate, and clinically-translatable methods of measuring pH in-vivo. Such technologies could be used as diagnostic, predictive, and/or response biomarkers, have been comprehensively reviewed elsewhere 11 . A hallmark of cancer is that tumors are highly heterogeneous at the genomic, anatomic, physiologic, and metabolic levels. The proximal cause of this heterogeneity is the “chaotic” and abnormal tumor vasculature, which leads to different microenvironments with different perfusion characteristics 12 . These perfusion deficits can lead to profound deprivation of nutrients and substrates, such as amino acids, glucose, and oxygen. In oxygen deprived (hypoxic) environments, cells must rely on fermentative glycolysis, i.e. the non-oxidative conversion of glucose to lactic acid, induced via the “Pasteur Effect”, to meet their energy demands. It is axiomatic that combined oxygen and glucose deprivation cannot be corrected, and cells will die of necrosis. Necrotic core are commonly observed radiographically in clinical cancers, and this is related to profound perfusion deficits 13 , 14 . However, in malignant cancer, fermentative metabolism occurs even under well oxygenated conditions, known as “aerobic glycolysis” or the “Warburg Effect”. This glycolytic switch is the result of hardwiring the stability of hypoxia inducible transcription factors, HIF-1 (and/or HIF-2), a condition known as “pseudohypoxia” 15 . This phenotype is selected early in carcinogenesis and likely provides a selection benefit of increased fitness 16 , 17 . The mechanisms driving aerobic glycolysis are not known and remain controversial, as do the fitness advantages of constitutively expressing pseudohypoxic phenotype. However, it is an unequivocal fact that tumors exhibiting a Warburg Effect consume significant amounts of glucose and produce copious amounts of non-oxidized (lactic) acids as a result. Indeed, recent evidence has suggested a strong correlation between glucose uptake, measured by FDG PET imaging, and acidosis measured with CEST MRI 18 . Acidosis is also exacerbated by poor perfusion, as pH decreases with increasing distance from blood vessels 19 . This results in the accumulation of acids in the extracellular environment and an acidic tumor pHe, with values as low as pH 6.5 20 – 23 . The elevated levels of extracellular lactate can be a nutrient source for other cancer or stromal cells in the tumor. Lactate is taken up by cells through monocarboxylate transporters; primarily MCT-1 and/or MCT-4 and utilized for energy production through oxidative metabolism ( Figure 2 ). Although MCT-4 is commonly thought to be responsible for lactate efflux, and MCT-1 for lactate influx, in fact both of these transporters are non-electrogenic permeases and respond to the immediate concentrations of lactate and H+ on either side of the membrane 24 . A recent and study using 13 C labeled precursors in human tumors has shown unequivocally that lactate produced in one region of a lung tumor can be a major fuel source for other cells in the tumor and that the uptake was mediated via MCT1 25 . Similarly, the conversion of 13 C pyruvate to 13 C lactate (and vice versa) can be monitored in human patients using dynamic nuclear hyperpolarized magnetic resonance imaging 26 . This release of acid by fermentative cells, results in profound acidity in areas that are proximal to the cell membrane. A recent study using Raman spectroscopy with gold nanoparticles reported an NHE dependent extremely low pH (~6.0) within 20 nM from the plasma membrane of tumor cells 27 . This information would be helpful for design of pH-dependent drug development. It is axiomatic that cancer cells must adapt to living in acidic pH in order to survive and thrive. These adaptations eventually make cancer cells more fit than the normal stromal cells that are their competitors. Because cultured cells are adapted to growth in alkaline media, switching them to a more acidic medium invariably slows their growth. However, cancer cells eventually adapt to growth in acidic media. As discussed below, this adaptation is pleotropic. There are significant metabolic adaptations to growth in acidic media ( Figure 3 ). At the genomic level, acidosis can induce genome instability through chromosome breakages and translocations driving somatic evolution 28 . Acidosis in the microenvironment provides a strong evolutionary selection pressure that contributes to the emergence of aggressive, therapy resistant clones 29 . A very common response to acidosis is a cessation of glucose fermentation and an increase in respiration fueled by glutamine consumption or beta-oxidation (β-ox) of fatty acids 30 . Increased oxidative flux increases reactive oxygen species 31 , and increased accumulation of the highly reactive acetyl CoA, which has been shown to non-enzymatically acetylate complex I, which restrains β-ox. Acid adaptation also leads to sirtuin-mediated histone deacetylation, which downregulates AcetylCoA carboxylase ACC2, leading to reversal of FAS inhibition and driving fatty acid synthesis that can paradoxically occur simultaneously with β-ox 30 . This may be related to the significant increase in the abundance of adiposomes, which is a rapid and reversible response to acidosis 32 . One of the most profound adaptations to acidosis is an increase in lysosomogenesis and re-distribution of lysosomes from a peri-nuclear location to the plasma membrane, where they can fuse and release their contents (including proteases and H + ) to the surrounding microenvironment 33 , 34 . This is hypothesized to contribute to ECM remodeling and local invasion 35 , 36 . This is also associated with redistribution of the lysosomal protein LAMP2 to the plasma membrane where it protects from acid hydrolysis 34 . LAMP2 is also a key particpant in inducing autophagy and acid-adapted cells are known to be chronically autophagic 37 . The resdistribution of lysosomes has also been associated with separation of mTORC from it regulatory complex, including RHEB 38 , and this may contribute to the metabolic changes mentioned above. Although its importance is not well-established, it is also known that acid adaptation is associated with an increase in the release of extracellular vesicles (EVs) by tumor cells 39 , 40 . It has been speculated that EVs are mediators of cell-to-cell communication 41 and indeed, acidosis has been shown to stabilize and increase exosomal RNA and protein content 42 . In a recent study, precise quantification of exosome release under different pH conditions was demonstrated in a number of cell lines derived from cancers of colon, breast, prostate, melanoma and osteosarcoma 43 . The results obtained using nanoscale flow cytometry or tracking analysis showed that that cells grown in acidic pH (6.5) release, on average, 4.6-fold more exosomes than the same cells grown in physiological pH (7.4) medium. pH dependence of exosome release was further demonstrated by progressively increasing the pH from 6.5 or decreasing the pH from 7.4. Cells grown in acidic media showed an increase in the activity of the endosomal compartment as seen by CD63 staining leading, in turn, to increased EV formation and release. Acidosis is a potent stimulator of local invasion ( Figure 4 ). Indeed, it has been shown that invadopodia contain proton-exporting machinery, which can either include NHE1 or a complex of NBCn1 coupled to CA9 44 , 45 . This establishes an alkaline intracellular pH (pHi) at the leading edge, with a more acidic pHi near the tailing end of the cell, leading to directional migration. The acidic extracellular pH at the leading edge can activate proteases, such as lysosome-released cathepsins. In tumors, cells at the invading edge express significantly more CA9 than those in the tumor core, prompting many to hypothesize that this distribution facilitates acidification of the invasion front 46 , 47 . Consistent with this, we have observed with intravital microscopy that invading tumors secrete acid into their surrounding stroma 48 , 49 , which induces ECM remodeling and local invasion. Tumor-derived acidosis has also been shown to promote tumor progression via inhibition of T-cell activation and induction of a macrophage phenotypic switch towards an M2 polarized phenotype 50 – 52 . While the exact mechanisms by which acid pH inhibits the effector function of tumor infiltrating lymphocytes, it is well established that acidosis results in reduced secretion of IFN-g and IL-2, up-regulation of CD25, and activation of STA5/ERK signaling 53 – 55 . Harold Dvorak famously characterized tumors as “wounds that do not heal” 56 . One component of the wound response is a transient ischemia-driven tissue acidification, which resolves as the wound heals 57 . The role of acidification in physiological wound healing is not known with certainty, but recent data suggest that acidification stimulates production of inflammatory cytokines by the stroma or endothelium 58 . As these induce neo-angiogenesis, the acidosis is reduced and inflammation resolves. In tumors, this acidification never resolves. Multiple mechanisms have been identified that underlie intrinsic and acquired chemoresistance: these include impaired drug uptake, increased drug efflux, deletion of receptors, altered drug metabolism, quantitative and qualitative alterations in drug targets, increased DNA damage repair and various anti-apoptotic mechanisms. The rapid efflux of anti-cancer drugs mediated by multidrug transporters and the partial or complete reversibility of chemoresistance combined with the absence of genetic mutations suggests a multifactorial process. However, a growing body of recent evidence suggests that chemoresistance can also be triggered by the highly acidic microenvironment of tumors. A large number of drugs, including conventional chemotherapeutics and more recent biological agents, are weak bases that are quickly protonated and are sequestered through the well-known phenomenon of “ion trapping” in acidic environments such as the extracellular microenvironment and the acidic organelles of tumor cells. It is therefore essential to develop new strategies to overcome the entrapment and neutralization of weak base drugs. As described below, one such strategy is to directly increase the pH of the tumor microenvironment. A deal of preclinical evidence on the ability of both buffers and proton exchange inhibitors to improve the effectiveness of anti-cancer drugs have supported clinical trials in both human patients and animals with spontaneous tumors whose results are discussed below. Tumor acidity is associated with cancer progression, and poor outcomes. Preclinical and some clinical studies have shown that targeting acidity can improve therapy responses. Hence targeting tumor acidosis is a relevant therapeutic target, and we describe herein four approaches for targeting acidosis: 1) direct targeting to neutralize tumor acid directly; 2) targeting metabolic vulnerabilities revealed by acidosis, 3) acid-activatable drugs and nanomedicines, and 4) inhibiting metabolic processes responsible for generating acids in the first place ( Figure 4 ). The most direct approach to target tumor acidity is to neutralize it through the administration of oral buffers, such as NaHCO 3 . It has been shown that mice can thrive on buffered drinking water with, e.g. ad lib 200 mM sodium bicarbonate or THAM, and that these treatments specifically increase tumor pH without affecting systemic pH balance. The specificity for tumors can best be understood in light of the fact that tumor pH is acidic and unregulated, whereas systemic pH is alkaline and highly regulated 59 . Hence, buffers act to bring tumor pH to be consistent with that of the rest of the body. In multiple studies, oral buffers have been shown to not affect growth of primary tumors but to significantly prevent metastases 60 , 61 and that these treatments can reduce the aggressiveness of spontaneous genetically engineered mouse cancer models (GEMMs) 62 , 63 . However, it has proven difficult to translate these findings to the clinic, as three clinical trials with sodium bicarbonate were pursued and failed to reach their dose targets due to poor compliance (taste) and moderate SAEs 64 . An alternative to directly target tumor acidity is provided by L-DOS47 (Helix Biopharma). L-DOS47 is a Jack Bean urease targeted with camelid antibodies to CEACAM6 antigen, which is overexpressed in a number of cancers 65 . Once at the target, the urease converts endogenous urea to 2 NH 4 + and 1 HCO 3 − , producing a net local increase in pH. This has been well-tolerated in phase I/II studies in NSCLC ( NCT02309892 ) 66 . Alternatively, TRC101 is an orally available HCl absorbing and non-digested micron sized particle (buffer) that has been used to treat patients with chronic kidney disease and shown to induce compensated metabolic alkalosis, which is the target 67 . It has just completed a phase III ( NCT03317444 ), but has not yet been investigated in cancer. As described above, adaptation to acidic microenvironment involves significant metabolic re-programming. The fact that metabolic pathways under acidosis are different than those under neutral pH can be exploited, as these present vulnerabilities that can be targeted. This was directly tested by Persi et al. who predicted the pH profiles of all intracellular enzymes through homology modeling, and used this information to identify enzymes whose activities would be crucial for survival at low, but not neutral, pH 68 . In their study, these vulnerabilities were validated with siRNA knockdowns, but pharmacological agents are available for two of the most vulnerable enzymes: GAPDH and G6PD. Glyceraldehyde Phosphate dehydrogenase, GAPDH, is a rate limiting enzyme in glycolysis that has been demonstrated to have translational potential in combating tumor growth. Several inhibitors including arsenate, arsenic trioxide, 3-bromopyruvate, iodoacetate, and many natural compounds such as Koningic acid (KA) are implicated to have anti-GAPDH activity. Koningic acid (KA) also known as heptelidic acid, is a sesquiterpene lactone isolated from soil fungi that directly dock/bind to the active site of human GAPDH 69 . Using machine learning, pharmacogenomics and metabolomics, a recent study demonstrated that cytotoxic effect of KA treatment is heterogeneous and is determined by the quantitative extent of Warburg Effect; glucose uptake and lactate secretion 70 . This study extended the concept of synthetic lethality to glycolytic/ acidic tumors by demonstrating that during the WE, the rate-controlling steps in glycolysis are different than in fully oxidative energy metabolism. Thus, pharmacological interventions using KA have the potential to specifically disrupt metabolic pathways important in neoplastic settings, but render healthy tissue largely unaffected 71 , 72 . Glucose-6-phosphate dehydrogenase (G6PDH) is a critical enzyme conferring pH sensitivity. Polydatin is a natural glucoside and a precursor to Resveratrol that has long been used in traditional Chinese medicine for many purposes, including anti-cancer properties 73 . Polydatin has been used as an anti-cancer agent for years, without knowledge of its biochemical target 74 . Recently, it has been shown to be a potent inhibitor of G6PD, the rate limiting step to enter the Pentose Phosphate Pathway, PPP 75 . In this study, it was shown that polydatin limited NADPH production via the PPP and led to toxic oxidative ER stress and that these effects could be mitigated by overexpression of G6PD. Further, it is well-tolerated in vivo and a phase II trial (albeit not in cancer) has been completed 76 . Genetic or pharmacologic inhibition of PGM1(phosphoglycerate mutase enzyme 1), that catalyze 3-phosphoglycerate (3PG) to 2 PG, has also shown to inhibit PPP flux and tumor growth in preclinical studies 77 . Development of agents that are only active under relatively acidic conditions is an area of active investigation. These include agents that are acid-labile and deliver therapy selectively to acidic microenvironment, as well as agents that are activated under acidic microenvironments. Acid-labile agents include nanoparticles, and labile linkers on antibody drug conjugates, ADC. Acid-labile nanoparticles employ multiple different platforms and chemistries intended to dissolve in mildly acidic conditions found in tumors, and this rapidly evolving field has been the subject of many reviews 78 – 80 . Notably, many of these contain either PET or activatable MR imaging moieties that allow monitoring of targeting and activation 81 , 82 . Antibody drug conjugates are designed to target cell surface receptors, and release their chemotherapeutic drugs following cleavage of an acid-labile linker on the cell surface or in an endosome. There are currently four approved ADCs for cancers and there are currently about 60 ADCs in clinical trials 83 . The chemistries of acid labile linkers has a long history and continues to evolve in order to fine tune the kinetics and pH profile of these linkers 84 , 85 For acid-activated agents, Engelman and colleagues have developed a series of peptides, called ”pH low inserting peptides” or pHLIPs, whose configuration changes under mildly acidic conditions and promotes the insertion of the peptide stably across the plasma membrane 86 . Notably, there is tremendous flexibility to tune the pH at which these are activated and the payloads that they contain, which can be imaging moieties, therapeutic agents, or both 87 . The most well-developed acid-activated agents, and the ones with the most promise for clinical translation, however, are the so-called Proton Pump Inhibitors, PPIs. PPIs (i.e. omeprazole, esomeprazole, lansoprazole, pantoprazole and rabeprazole) are used worldwide as very potent antacids. These are over-the-counter medications and are very well tolerated, even with chronic treatments 88 , 89 . PPIs are Tetracyclic Sulfenamides that activated by protonation to become sulhdryl (e.g. cysteine) reagents. They were developed to be activated by stomach acid and bind irreversibly to and inhibit the gastric H + /K + ATPase. In cancer cells, PPIs have been shown to increase the pH of lysosomes due to inhibition of V-ATPase, and targeting in an acidic mileau may be due to the increased lysosomal-endosomal turnover, described above 90 , 91 . PPis may be used a monotherapy or in combination with chemo- or immune-therapies with improved responses 52 , 92 , 93 . Similar observations were made in clinical studies in household pets with advanced or chemo-refractory tumors, where PPIs achieved long term responses with improved performance status when combined with either standard treatment 94 or metronomic regimens 95 . These studies carry a significant translational value considering that cancers in companion animals are spontaneous and can share many similarities with human tumors 96 . Notably, cancer is the principal cause of death in pet dogs, with an incidence among certain breeds such as Golden Retrievers and Bernese Mountain Dogs approaching an incidence of 50% 97 . In dogs and cats, the progression of cancer is extremely rapid, with an aggressive behavior that frequently results in poor responses to therapy. This could be partially ascribed to their compressed lifespan compared to humans, however, if we consider this from a metabolic point of view, it could be induced and influenced by a baseline metabolism that is more acid than that of herbivores and primates. In particular, it has been underlined by a recent work that the gastric pH of dogs and cats is much more acid than omnivorous and herbivorous species, potentially laying the base for an acid milieu favoring the occurrence of neoplasia. Likewise, in terms of incidence, cancer is infrequently reported in horses, accounting for less than 5% of the surgeries performed at referral institutions 98 , compared to dogs whose incidence, accordingly to the cancer registries, is around 45%, despite being their lifespan much shorter than horses’ 99 .This could also explain the extreme effectiveness of alkaline therapy in such species when combined to conventional chemotherapy 100 . Because of their widespread use, there are a number of population-based studies showing beneficial effects of PPIs in the management of cancer. A recent observational case-control study accrued 64,234 women diagnosed with breast cancer between 2004 and in 2013 selected as cases and an equal number of healthy women as controls. Logistic regression modeling analysis revealed breast cancer patients were 25% less likely to have had prior PPI exposure 101 . A dose-response effect was also detected, with the highest effect, 35% lower PPI odds (95%CI 0.61–0.70) among patients in the highest exposure category suggesting that women at a higher-than-average risk of breast cancer may benefit from PPI prescriptions if they have medical conditions that could benefit from PPIs. The high safety profile, low cost and widespread long term usage of PPIs makes them ideal candidates for further exploration into their anticancer effects. A retrospective meta-analysis of 596 previously untreated head and neck squamous cell carcinoma (HNSCC) patients revealed a strong univariate association between PPIs use and improved overall survival (P<0.001) 102 . A retrospective analysis of patients with refractory GI cancer showed that the addition of PPI to chemotherapy significantly increased the time to progression 103 . The “reverse pH gradient (acid outside, alkaline inside)” in tumors is maintained by increased expression and/or activity of various plasma membrane transporters and acid efflux proteins that control pH homeostasis, including vacuolar-type H + -ATPase, monocarboxylate transporters (MCTs), Na+–H+ exchangers (NHEs), and carbonic anhydrases (CAs) 1, 104 – 108 ( Figure 1 ). Disrupting pH homeostasis by inhibiting these transporters and exchangers has been suggested as a therapeutic strategy and some of these inhibitors are in clinical trials 109 , 110 . While NHE1 is ubiquitously expressed in cancer and normal cells, it appears to play more of an essential role in cancers. For example, knocking out NHE1 has a greater impact on tumor growth if combined with mutations that increase the lactic acid load 111 . Further, silencing of NHE1 or MCT4 expression reduced of the pH gradient and limited tumor growth in similar xenograft models 112 , 113 . Amiloride, the first NHE inhibitor developed was shown to affect metastatic process by decreasing vasoendothelial growth factor (VEGF) production and the activity of urokinase-type plasminogen activator (μPA), metalloproteinases (MMP) and other proteases 114 . Since then, more potent and specific NHE1 inhibitors have been developed (e.g. ethylisopropyl- Hexamethyl- or dimethyl- amilorides) 115 , 116 . These potassium sparing diuretic have antineoplastic and anti-metastatic properties and are well tolerated and safe 117 – 120 . In clinical trials, cariporide, a non-amiloride based NHE1 inhibitor provided protection to the myocardium during ischemic-reperfusion injury, yet had a small therapeutic window 121 . However, there is potential for repurposing cariporide as an anti-cancer agent as it is very effective in initiating internal acidification of cancer cells by inhibiting NHE1 mediated H + efflux leading to cancer cell death 121 – 123 . The challenge with using NHE1 inhibitors` is to identify rational combinations that will increase the therapeutic efficacy. Monocarboxylate transporters (MCTs) are crucial players in regulating pH homeostasis by facilitating the export of lactic acid from glycolytic cancer cells or to facilitate uptake of lactate or pyruvate as energy sources. Among the 14 isoforms identified, MCT1 and 4 are lactate/H+ symporters broadly expressed in cancers and are associated with cancer aggressiveness and prognosis in many cancer types 124 – 126 . Although it is thought that MCT1 is mainly involved in lactate uptake and MCT4 in lactate export, it is becoming increasingly appreciated that these permeases primarily respond to the lactate, pyruvate, and H+ concentrations on each side of the membrane 119 , 127 , 128 . Specific siRNA silencing of MCT1 in malignant human glioma cells demonstrated a decrease of pHi by 0.6 units and rapid cell death, confirming the key role of MCTs in pHi regulation 129 . Although the first generation small molecule MCT inhibitors, the cinnamates, were effective in lowering intracellular pH and cell viability, they were not specific enough for further clinical development 24 , 130 , 131 . Blocking MCT1/2 in Ras-transformed fibroblast CCL39 cells expressing MCT1 and 2 with AR-C155858, a specific MCT1/2 inhibitor developed by Astra Zeneca, suppressed lactate export, glycolysis, and strongly reduced pHi and cell growth when cells were forced to use glycolysis in response to oligomycin treatment. In addition, ectopic expression of MCT4 in these cells conferred resistance to MCT1/2 inhibition and reestablished tumorigenicity, stressing the role of MCT4 in pHi regulation 132 . This was further confirmed using 31P magnetic resonance spectroscopy by overexpressing MCT4 in Ras transformed cells, showing that intracellular pH was elevated to alkaline levels while extracellular pH was acidic 112 . Moreover, MCT4-depleted breast cancer cells showed increased cell death and reduced tumor growth due to elevated levels of reactive oxygen species and decreased intracellular pH 133 . AZD3965, a more specific and potent inhibitor of MCT1 derived from AR-C155858 has shown promise in preclinical studies of small cell lung cancer, colon cancer and lymphoma 134 – 137 . As expected, the major metabolic consequences of MCT1 inhibition were lactate accumulation, decrease in intracellular pH as well as dependence on mitochondrial metabolism which sensitized the cells to mitochondrial complex 1 inhibitors, metformin or phenformin. AZD3965 is currently undergoing phase I/II clinical trials in the UK for patients with solid tumors, prostate cancer and diffuse large-cell B lymphoma 138 , 139 . In addition, syrosingopine, an antihypertensive drug, is reported to be a dual inhibitor of MCT1 and MCT4 and elicited synthetic lethality with metformin due to NAD + depletion in cancer cells 140 . Interestingly, in a recent study, MCT1/2 inhibitor (SR13800) was successfully employed to lower intracellular pH and was efficient in inhibiting proliferation in breast cancer cells when combined with depletion of GAPDH. This synthetic lethal approach was particularly effective in breast cancer cells adapted to hypoxia or low extracellular pH and that display aggressive phenotypes 68 . Taken together, these studies strongly suggest the potential of therapeutically targeting metabolic vulnerabilities under acidosis. Carbonic Anhydrase IX is an attractive target for the purpose of reducing tumor acidity. As described above, CA9 is a major contributor to extracellular acidosis, it is a negative prognostic marker in many cancers, and is strongly associated with increased invasion and metastasis 141 , 142 . Further, its normal tissue expression is restricted to the upper GI tract and gall bladder ( www.proteinatlas.org ). Because of its attractiveness as a target, it has been a drug target since its discovery by Pastorek and Pastorekova in 1994 143 . In general, CA inhibitors contain sulfonamide groups that target the active site 144 . Exofacial CAs, such as CA9 and CA12, can be selectively targeted by appending a large hydrophilic or halogenated group to reduce internalization 145 . The status of CA9 and CA12 inhibitors and their role as anti-cancer agents has recently been reviewed 146 , 147 . Importantly, cancer cells adapted to acidosis are particularly susceptible to CA9 inhibition 148 . In vivo, CA9 knockdown or specific CA9 inhibitors (CAI17) similarly suppressed tumor growth and metastasis in aggressive breast tumor models (4T1, MDAmb231) 149 . A CA9/CA12 specific inhibitor SLC0111 was additive to temozolomide in delaying growth of GBM in vivo 150 . A phase I trial of SLC0111( NCT02215850 ) was completed last year, and a new trial is set to open in combination with gemcitabine in pancreatic cancer ( NCT03450018 ). It has almost been a century since the Nobelist, Otto Warburg, first described the phenomenon of aerobic glycolysis in cancers and he, at that time, postulated that this would result in acid-base imbalances. It has only been in the last quarter century that tools have been developed with which to interrogate the acid/base balance of tumors. It has only been in the last decade, that it is has become more widely appreciated that solid tumors (and even those residing in bone marrow) can be profoundly acidic and that, on one hand, adaptation to this acidosis provides the cancer cells with an evolutionary fitness advantage, but on the other hand, this also exposes them to therapeutic vulnerabilities. Herein, we have described some of these adaptations and therapeutic approaches to either reduce the acidosis itself to eliminate the fitness advantage, or to exploit the acid-induced vulnerabilities.