In-vitro study to evaluate anti-oxidant, diabetic and inflammatory potentials of rhizomes of Zingiberaceae

ABSTRACT Diabetes control relies heavily on very pricy medical care, which has emerged as a major global health concern. As a result, it is imperative to look at potential alternatives to treatment, the cost-effective ways and reduce side effects on our health from synthetic drugs. Purpose of this study mainly emphasizes on evaluation of rhizomes of twenty three plants of the family Zingiberaceae for the presence of active phytochemicals that inhibiting anti-diabetic, inflammatory, and oxidant activities. Anti-oxidant effect of these extracts were examined by Reducing Power, DPPH, and ABTS assays. The methanolic extract showed potent anti-oxidant activity which is comparable to ascorbic-acid and trolox used as control standard. While anti-diabetic activity was evaluated based on α-glucosidase and α-amylase enzymes inhibition assays with acarbose drug act as standard. Out of the 23 samples,10 samples were showed significant (IC50 values< 100 μg/mL) by α-glucosidase inhibitory effect inclusive of which rhizomes of Alpinia calcarata had the most optimum activity in-vitro with IC50 values 2.58±0.16 μg/mL for α-glucosidase and IC50=145.8±0.09 μg/mL for α-amylase enzymes assays. Further, anti-inflammatory potential of these extracts were performed using Heat-induced and Hypoxicity-induced hemolysis methods with Indomethacin used as standard. Entire aforementioned activities assay gives significant results for respective methanolic extracts. Using standard deviation, the results were determined to be statistically significant (p<0.005).