Ultrasound Assisted Extraction of Micro and Macroelements in Fruit Peels Powder Mineral Supplement for Osteoporosis Patients and their Determination by Flame Atomic Absorption Spectrometry

Osteoporosis is a worldwide disease depicted by the reduced bone mass, an adequate supply of minerals are needed to support bone remodelingand their deficiency causes bone-related diseases, osteoporosis in particular, and have osteo-protective effects. The aim of recent researchwas to quantify the micro (Mn, Fe, Cu, and Zn) and macroelements (Mg, K, and Ca) in the peels powder of some common fruits (pomegranate, orange, lemon, mango, and grapefruit) by flame atomic absorption spectrometer (FAAS). The extraction of micro and macroelements in peels powder was done by using dilute acids in ultrasonic bath. Apple leaves were used as standard reference material (SRM, NIST 1515) to optimize the ultrasound assisted extraction (UAE) method at varied operating parameters. Maximum response was obtained for extracting of minerals in 500 mg SRM at 60 ° C temperature, setting a vortexing time of 5 min.while using 5.0 mL extracting agent HNO 3 (0.5 M)-H 2 O 2 (10 %) at90 % sonication amplitude of ultrasound bath for 6 min.While analyzing the SRM, the percentage recovery wasobtained in ranged between 96.8-102.7 % to assure the accuracy whereasrepeatability (n = 10) study in terms of % RSDyielding≤ 2.29well support the precision of proposed method and limits of quantitation (µg/g) were 0.034, 0.061, 0.065, 0.057, 0.017, 0.175 and 0.053 for Mn, Fe, Cu, Zn, Mg, K and Ca respectively. The proposed UAE method was reliable,efficient, and advantageous over the conventionally employed acid digestion method with regards to less consumption of reagents and short analysis time for the determination of micro and macroelements in fruit peels powder.