Central nervous system depressant outcomes of Artemisia monosperma Delile on male rat brain: Phytochemical analysis

Central nervous system depressant outcomes of Artemisia monosperma Delile on male rat brain: Phytochemical analysis | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Central nervous system depressant outcomes of Artemisia monosperma Delile on male rat brain: Phytochemical analysis Ehsan Abu Zeid This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-5338696/v2 This work is licensed under a CC BY 4.0 License Status: Posted Version 2 posted You are reading this latest preprint version Show more versions Abstract The current study aimed to examine the neurobehavioral activity of Artemisia monosperma Delile (ARM) ethanolic extract in adult male rats. Phytochemicals was screened using GC˗MS and HPLC analysis. ARM’s acute oral toxicity was determined. The study included four equal groups (n=12): the 1 st Control, the 2 nd Diazepam (DIZ), the 3 rd ARM-high group (ARM-H), and the 4 th ARM-low group (ARM-L). The ARM extract effect on the CNS activity was evaluated via behavioral tests, brain neurotransmitters and GABA A receptors ( GABARAP) . HPLC analysis revealed the presence of coffeic acid, rutin, gallic acid, and querectin. In the open field test (OFT), ARM significantly (p < 0.001) decreased immobility time and line crossings. ARM significantly (p < 0.05) increased the brain γ-aminobutyric acid (GABA). ARM significantly (p < 0.05) up-regulated the brain mRNA expression levels of GABARAP and BDNF , meanwhile, MAOA levels were significantly (p < 0.05) downregulated. For the first time, A. monosperma ethanolic extract might keep CNS depressant and antioxidative effects which could be attributable to its phytochemicals. Further toxicological and pharmacological studies are required for the semi-purified phytochemicals. Central nervous system activity Artemisia monosperma GABA type A receptor mRNA expression GC˗MS and HPLC analysis Figures Figure 1 Figure 2 Figure 3 Full Text Additional Declarations The authors declare no competing interests. Cite Share Download PDF Status: Posted Version 2 posted You are reading this latest preprint version Show more versions Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-5338696","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":392440800,"identity":"f1a8a207-6241-4f2a-8244-6fef1c1b4bfe","order_by":0,"name":"Ehsan Abu Zeid","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAABAklEQVRIie3RMUvEMBTA8RciuLRkTQn0M7zp8FD0q1QK5xI5dwcPhNzSc67ol3A5HFMCuhRcO3qLk2LBpT0q2HbQW6J1E8x/SXjw4w0PwOX6i3Egmsy6t63sR9GPBD4JSX9NqDeEMHGus/p2fxrM85HYawywbYlQru0kuL6LjJ/H4ytPjoT0DATJM5LLCzvBQqIhimIIx0shuekn1E/s5KCYllmtzjBkL0uxg6adtOT9G4JcgvaVQcHbLRCZboIUKjvhxQSNr+4xSF/fxok+8nj+dJItZnbC0ni1qtUp8ofJYVE1uyGbxzePVWMnm211t+lPo4kaRmj59R+4xeVyuf5FH+xqVL5hzvdJAAAAAElFTkSuQmCC","orcid":"","institution":"Zagazig University","correspondingAuthor":true,"prefix":"","firstName":"Ehsan","middleName":"Abu","lastName":"Zeid","suffix":""}],"badges":[],"createdAt":"2024-10-26 18:08:07","currentVersionCode":2,"declarations":{"humanSubjects":false,"vertebrateSubjects":false,"conflictsOfInterestStatement":false,"humanSubjectEthicalGuidelines":false,"humanSubjectConsent":false,"humanSubjectClinicalTrial":false,"humanSubjectCaseReport":false,"vertebrateSubjectEthicalGuidelines":false},"doi":"10.21203/rs.3.rs-5338696/v2","doiUrl":"https://doi.org/10.21203/rs.3.rs-5338696/v2","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":72294641,"identity":"5fdf1a59-31db-41d6-a281-c4fec055e599","added_by":"auto","created_at":"2024-12-24 19:14:41","extension":"jpg","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":551460,"visible":true,"origin":"","legend":"\u003cp\u003eEffect of DIZ or ARM high and low doses administration on neurotransmitters levels in adult male rat brain. A. Gamma amino butyric acid (GABA). B. Serotonin (5-HT). C. Dopamine (DA).\u003c/p\u003e","description":"","filename":"Figure1.jpg","url":"https://assets-eu.researchsquare.com/files/rs-5338696/v2/e20294188ca1ba80a74253b0.jpg"},{"id":72294766,"identity":"e72def98-c007-4520-af90-39769127ce37","added_by":"auto","created_at":"2024-12-24 19:22:41","extension":"jpg","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":615915,"visible":true,"origin":"","legend":"\u003cp\u003eEffect of DIZ or ARM high and low doses administration on oxidative stress-related parameters in adult male rat brain. A. Total antioxidant capacity (TAC). B. Malondialdehyde (MDA). C. Protein carbonyl (PCO). D. 8hydroxy2deoxyguanosine (8OH2dG).\u003c/p\u003e","description":"","filename":"Figure2.jpg","url":"https://assets-eu.researchsquare.com/files/rs-5338696/v2/e3edfdd5503b8223b72707d0.jpg"},{"id":72294640,"identity":"0011a764-3333-4389-973b-d13d74139642","added_by":"auto","created_at":"2024-12-24 19:14:41","extension":"jpg","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":538761,"visible":true,"origin":"","legend":"\u003cp\u003eEffect of DIZ or ARM high and low doses administration on relative mRNA expression levels of brain mRNA expression levels of GABARAP, BDNF, and MAOA in adult male rat brain. A. mRNA expression of GABA type A receptor-associated protein (GABARAP). B. mRNA expression of brain-derived neurotrophic factor (BDNF). C. mRNA expression of Monoamine oxidase A (MAOA).\u003c/p\u003e","description":"","filename":"Figure3.jpg","url":"https://assets-eu.researchsquare.com/files/rs-5338696/v2/bbafdc18ca17d8e92b16ea21.jpg"},{"id":72295337,"identity":"da96a1a7-efb0-4bb7-9337-4c03c74b934a","added_by":"auto","created_at":"2024-12-24 19:38:44","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":2147369,"visible":true,"origin":"","legend":"","description":"","filename":"ManuscriptNew.pdf","url":"https://assets-eu.researchsquare.com/files/rs-5338696/v2_covered_c298658d-18ed-4f67-a455-41be4b995946.pdf"}],"financialInterests":"The authors declare no competing interests.","formattedTitle":"\u003cp\u003e\u003cstrong\u003eCentral nervous system depressant outcomes of\u003c/strong\u003e\u003cem\u003e\u003cstrong\u003e Artemisia monosperma Delile\u003c/strong\u003e\u003c/em\u003e\u003cstrong\u003e on male rat brain: Phytochemical analysis\u003c/strong\u003e\u003c/p\u003e","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Central nervous system activity, Artemisia monosperma, GABA type A receptor mRNA expression, GC˗MS and HPLC analysis","lastPublishedDoi":"10.21203/rs.3.rs-5338696/v2","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-5338696/v2","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eThe current study aimed to examine the neurobehavioral activity of\u003cem\u003e Artemisia monosperma Delile \u003c/em\u003e(ARM) ethanolic extract in adult male rats. Phytochemicals was screened using GC˗MS and HPLC analysis. ARM’s acute oral toxicity was determined. The study included four equal groups (n=12): the 1\u003csup\u003est\u003c/sup\u003e Control, the 2\u003csup\u003end\u003c/sup\u003e Diazepam (DIZ), the 3\u003csup\u003erd\u003c/sup\u003e ARM-high group (ARM-H), and the 4\u003csup\u003eth\u003c/sup\u003e ARM-low group (ARM-L). The ARM extract effect on the CNS activity was evaluated via behavioral tests, brain neurotransmitters and \u003cem\u003eGABA\u003c/em\u003e\u003csub\u003e\u003cem\u003eA\u003c/em\u003e\u003c/sub\u003e\u003cem\u003e \u003c/em\u003ereceptors (\u003cem\u003eGABARAP)\u003c/em\u003e. HPLC analysis revealed the presence of coffeic acid, rutin, gallic acid, and querectin. In the open field test (OFT), ARM significantly \u003cem\u003e(p \u0026lt; 0.001) \u003c/em\u003edecreased immobility time and line crossings. ARM significantly \u003cem\u003e(p \u0026lt; 0.05)\u003c/em\u003e increased the brain γ-aminobutyric acid (GABA). ARM significantly \u003cem\u003e(p \u0026lt; 0.05)\u003c/em\u003e up-regulated the brain mRNA expression levels of \u003cem\u003eGABARAP\u003c/em\u003e and \u003cem\u003eBDNF\u003c/em\u003e, meanwhile, \u003cem\u003eMAOA\u003c/em\u003e levels were significantly \u003cem\u003e(p \u0026lt; 0.05)\u003c/em\u003e downregulated. For the first time, \u003cem\u003eA. monosperma\u003c/em\u003e ethanolic extract might keep CNS depressant and antioxidative effects which could be attributable to its phytochemicals. Further toxicological and pharmacological studies are required for the semi-purified phytochemicals.\u003c/p\u003e","manuscriptTitle":"Central nervous system depressant outcomes of Artemisia monosperma Delile on male rat brain: Phytochemical analysis","msid":"","msnumber":"","nonDraftVersions":[{"code":2,"date":"2024-12-24 19:14:36","doi":"10.21203/rs.3.rs-5338696/v2","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}},{"code":1,"date":"2024-10-30 12:35:57","doi":"10.21203/rs.3.rs-5338696/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"f5a30a06-e5ec-4172-98ac-aa601dcd9a36","owner":[],"postedDate":"December 24th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2024-10-30T12:35:59+00:00","versionOfRecord":[],"versionCreatedAt":"2024-12-24 19:14:36","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v2","identity":"rs-5338696","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-5338696","identity":"rs-5338696","version":["v2"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}