Historical collections of tropical marine mammals are an excellent resource for ancient DNA

Historical collections of tropical marine mammals are an excellent resource for ancient DNA | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 11 January 2025 V1 Latest version Share on Historical collections of tropical marine mammals are an excellent resource for ancient DNA Authors : Lydia Furness 0009-0003-0964-0215 [email protected] , Richard Sabin , Marianne Torvanger , Oliver Kersten , James Barrett , and Bastiaan Star Authors Info & Affiliations https://doi.org/10.22541/au.173657548.85754194/v1 Published Molecular Ecology Resources Version of record Peer review timeline 253 views 184 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract The ability to predict ancient DNA sequencing success in natural history collections is critical to reducing the amount of destructive sampling of a finite resource. So far, studies investigating such success have predominantly focused on taxa with ranges restricted to temperate or cold environments at northern latitudes which likely aids DNA preservation. Here, we report remarkably high aDNA sequencing success in Sirenia, herbivorous marine mammals of which the distribution is currently constrained to the global tropics. We investigate 91 samples from 85 specimens comprising all four contemporary species and one extinct species, comparing different sample types (cranial/post-cranial bone, skin and cartilage), species, collections, and material age. We obtained remarkably high (e.g. > 20%) endogenous DNA preservation for the majority (e.g. ~57% percent) of samples. Sequencing success was linked to sample type, with cranial bones (including petrous and tympanic bones) yielding significantly higher endogenous DNA. Additionally, we obtained variable, but potentially superior DNA results for preserved cartilage and hide samples that can be associated with historical bone. Although such tissue is not always present, this type of material is easy to sample, with very limited destructive impacts on the associated bones and we therefore highlight its untapped potential as a source of DNA. Overall, our results show the high success of ancient DNA retrieval from historical collections of species with a tropical distribution expanding on the types of specimens that are available for temporal genomic analyses. Supplementary Material File (endogenous_dna_draft.docx) Download 193.04 KB Information & Authors Information Version history V1 Version 1 11 January 2025 Peer review timeline Published Molecular Ecology Resources Version of Record 17 Jul 2025 Published Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords ancient dna destructive sampling historical dna natural history collections sirenia tropical marine mammals Authors Affiliations Lydia Furness 0009-0003-0964-0215 [email protected] University of Oslo Faculty of Mathematics and Natural Sciences View all articles by this author Richard Sabin Natural History Museum, London, UK View all articles by this author Marianne Torvanger University of Oslo Faculty of Mathematics and Natural Sciences View all articles by this author Oliver Kersten University of Oslo Faculty of Mathematics and Natural Sciences View all articles by this author James Barrett Norwegian University of Science and Technology View all articles by this author Bastiaan Star University of Oslo Faculty of Mathematics and Natural Sciences View all articles by this author Metrics & Citations Metrics Article Usage 253 views 184 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Lydia Furness, Richard Sabin, Marianne Torvanger, et al. Historical collections of tropical marine mammals are an excellent resource for ancient DNA. Authorea . 11 January 2025. DOI: https://doi.org/10.22541/au.173657548.85754194/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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