Comparative evaluation of dose dependent efficacy of vitamin C, in relation to oxidative stress level in subjects  with Oral Submucus Fibrosis

Background: Oral submucus fibrosis (OSMF) has a 0.2-0.5 prevalence rate in India. The malignancy rate of OSMF was found to be 7.6%. The focus of pathogenesis is currently on reactive oxygen species levels which can rise to extremely high levels, exceeding the human body’s antioxidant defense system and causing oxidative stress that goes beyond physiological limitations. Since oxidative stress plays such a significant part in carcinogenesis and other degenerative diseases, it is fair to believe that antioxidants will help to reduce or even stop these processes. Vitamin C is known for its antioxidant properties which act as a lipid-soluble free radical scavenger in cell membranes. But in some of studies a paradoxical effect of antioxidant doses was found. Thus, the present study will be conducted to evaluate the dose dependent efficacy of Vitamin C level in relation to oxidative stress level in subjects of OSMF. Methods After informed consent, the patient’s examination will be conducted. For each individual, 5ml of the blood sample will be withdrawn under all the aseptic precautions and oxidative stress level will be assessed depending on MDA & SOD level in a central research laboratory. Depending on oxidative stress level patients will be divided into two groups. Group I - Moderate oxidative stress in OSMF SI less than 240 but more than 192 Group II - High Oxidative Stress in OSMF SI more than 240 Single and double doses of Vitamin C will be given to patients accordingly and the MDA & SOD levels will be calculated on day 30,90 days and on 180th day Conclusions Positive results of the study will give direction for choosing the correct dose of the antioxidant Vitamin C for OSMF depending on oxidative stress level.