Guidance on the use of microscopy in the detection of zoosporic parasites of phytoplankton in natural systems and algal biotechnology

2024 · doi:10.3897/arphapreprints.e124344
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Abstract

The objective of this methodological paper is to outline a microscopy protocol for detecting and observing fungal zoosporic parasites of phytoplankton in natural environments, with potential applications in algal biotechnology to enhance the success and sustainability of algal cultivation endeavors. The fluorescent dual staining with wheat germ agglutinin (WGA) and Calcofluor White (CFW) showcased during the Training School "Identification and detection of zoosporic parasites" in Neuglobsow (Germany) from 16 th till 18 th of May 2023 and organized and funded by COST Action ParAqua (European Cooperation in Science and Technology) illustrated its ease of implementation and suitability for diverse natural and artificial systems.
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Preprint ARPHA Preprints https://doi.org/10.3897/arphapreprints.e124344 (01 Apr 2024) https://doi.org/10.3897/arphapreprints.e124344 (01 Apr 2024) Submitted to Research Ideas and Outcomes Other versions: - Preprint InfoPreprint Info - CiteCite - MetricsMetrics - CommentComment - RelatedRelated - CitedCited ARPHA Preprints doi: 10.3897/arphapreprints.e124344 First posted 01 Apr 2024 Authors Milos Stupar - Corresponding author University of Belgrade, Faculty of Biology, Belgrade, Serbia Institute of Materials Science of Mulhouse (IS2M), CNRS Mulhouse, Mulhouse, France Department of Biology, University of Turku, Turku, Finland Alfred Wegener Institute , Bremerhaven, Germany Institut de Ciències del Mar (CSIC), Barcelona, Spain Université Savoie Mont Blanc, INRAE, CARRTEL, Thonon les Bains, France Conflict of interest The authors have declared that no competing interests exist. This is an open access preprint distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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