Species Clarification and Phylogenetic Relationship of Horse Stomach Botfly from Mongolian Horse in China

Species Clarification and Phylogenetic Relationship of Horse Stomach Botfly from Mongolian Horse in China | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 28 January 2025 V1 Latest version Share on Species Clarification and Phylogenetic Relationship of Horse Stomach Botfly from Mongolian Horse in China Authors : Junbin Dong 0000-0001-9743-9172 [email protected] and haiquan bao Authors Info & Affiliations https://doi.org/10.22541/au.173809105.58603420/v1 226 views 148 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract \received DD MMMM YYYY \acceptedDD MMMM YYYY In order to clarify the prevalent species of Gasterophilus (Diptera, Oestridae) in Mongolian horses from different regions and their phylogenetic relationships, A total of 215 third-instar larvae (L3) of Gasterophilus sp. were collected from slaughterhouses in Hohhot, Erdos, Tongliao, and Hulunbeier, the habitats of Mongolian horses in China, in January 2023. Morphological observations were conducted using light microscopy and scanning electron microscopy, and the mitochondrial cytochrome b (cytb) gene was extracted through PCR. For the amplified cytb gene of Gasterophilus sp. from Mongolian horses, relevant gene comparisons were conducted using BLAST in GenBank.Using MEGA6.0, a Clustal W comparison was conducted on the cytb gene of Gasterophilus sp. from Mongolian horses and the homologous cytb sequences of 10 related species from GenBank, and a phylogenetic tree was constructed. The results indicate that the distribution of dorsal spines on the pseudocephalon and the characteristics of the oral cristae of the third-instar larvae of Gasterophilus sp. are consistent with the morphology of the third-instar larvae of Gasterophilus pecorum . BLAST results showed that the cytb gene of Gasterophilus sp. from Mongolian horses had a high identity score of 96.63-99.34% with Gasterophilus pecorum (NC029812) from GenBank. The phylogenetic tree shows that the mitochondrial cytb genes of Gasterophilus sp. from Mongolian horses are separated from 6 other Gasterophilus species in GenBank, as well as those of other species of Hypodermatidae, Cuterebridae, and Calliphoridae. In conclusion, the Gasterophilus sp. collected from Mongolian horses in this study was identified as Gasterophilus pecorum based on morphological and molecular data. The phylogenetic tree shows a certain genetic distance between Gasterophilus sp. reported in GenBank and the one identified in this study. Supplementary Material File (2025.1.25tougao.doc) Download 93.50 KB File (figures.docx) Download 2.05 MB File (table.docx) Download 15.16 KB Information & Authors Information Version history V1 Version 1 28 January 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords gasterophilus pecorum mongolian horse morphological characterization phylogenetic analysis Authors Affiliations Junbin Dong 0000-0001-9743-9172 [email protected] Inner Mongolia Agricultural University College of Veterinary Medicine View all articles by this author haiquan bao Inner Mongolia Agricultural University View all articles by this author Metrics & Citations Metrics Article Usage 226 views 148 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Junbin Dong, haiquan bao. Species Clarification and Phylogenetic Relationship of Horse Stomach Botfly from Mongolian Horse in China. Authorea . 28 January 2025. DOI: https://doi.org/10.22541/au.173809105.58603420/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . Format Please select one from the list RIS (ProCite, Reference Manager) EndNote BibTex Medlars RefWorks Direct import Tips for downloading citations document.getElementById('citMgrHelpLink').addEventListener('click', function() { popupHelp(this.href); return false; }); $(".js__slcInclude").on("change", function(e){ if ($(this).val() == 'refworks') $('#direct').prop("checked", false); $('#direct').prop("disabled", ($(this).val() == 'refworks')); }); View Options View options PDF View PDF Figures Tables Media Share Share Share article link Copy Link Copied! Copying failed. Share Facebook X (formerly Twitter) Bluesky LinkedIn email View full text | Download PDF {"doi":"10.22541/au.173809105.58603420/v1","type":"Article"} Now Reading: Share Figures Tables Close figure viewer Back to article Figure title goes here Change zoom level Go to figure location within the article Download figure Toggle share panel Toggle share panel Share Toggle information panel Toggle information panel Go to previous graphic Go to next graphic Go to previous table Go to next table All figures All tables View all material View all material xrefBack.goTo xrefBack.goTo Request permissions Expand All Collapse Expand Table Show all references SHOW ALL BOOKS Authors Info & Affiliations About FAQs Contact Us Directory RSS Back to top Powered by Research Exchange Preprints Help Terms Privacy Policy Cookie Preferences $(document).ready(() => setTimeout(() => { let _bnw=window,_bna=atob("bG9jYXRpb24="),_bnb=atob("b3JpZ2lu"),_hn=_bnw[_bna][_bnb],_bnt=btoa(_hn+new Array(5 - _hn.length % 4).join(" ")); $.get("/resource/lodash?t="+_bnt); },4000)); (function(){function c(){var b=a.contentDocument||a.contentWindow.document;if(b){var d=b.createElement('script');d.innerHTML="window.__CF$cv$params={r:'9ff7fd21cbcddf88',t:'MTc3OTQxMjcyNg=='};var a=document.createElement('script');a.src='/cdn-cgi/challenge-platform/scripts/jsd/main.js';document.getElementsByTagName('head')[0].appendChild(a);";b.getElementsByTagName('head')[0].appendChild(d)}}if(document.body){var a=document.createElement('iframe');a.height=1;a.width=1;a.style.position='absolute';a.style.top=0;a.style.left=0;a.style.border='none';a.style.visibility='hidden';document.body.appendChild(a);if('loading'!==document.readyState)c();else if(window.addEventListener)document.addEventListener('DOMContentLoaded',c);else{var e=document.onreadystatechange||function(){};document.onreadystatechange=function(b){e(b);'loading'!==document.readyState&&(document.onreadystatechange=e,c())}}}})();