Electrospun PAAm:Bi₂Fe₄O₉ Nanofiber Membranes with Iron-Mediated Charge Dynamics for Enhanced Antibacterial Performance: Structural and Mechanistic Investigations

Electrospun PAAm:Bi₂Fe₄O₉ Nanofiber Membranes with Iron-Mediated Charge Dynamics for Enhanced Antibacterial Performance: Structural and Mechanistic Investigations | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Electrospun PAAm:Bi₂Fe₄O₉ Nanofiber Membranes with Iron-Mediated Charge Dynamics for Enhanced Antibacterial Performance: Structural and Mechanistic Investigations Ahmed M. Ajam, Khalid Haneen Abass This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-9316369/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract This research investigates the preparation of polyacrylamide (PAAm) polymer-based nanofiber composites loaded with bismuth ferrite (Bi₂Fe₄O₉) nanoparticles using electrospinning technique. The effect of loading ratios on the structural, optical, and antibacterial properties was also investigated. The nanoparticles were prepared and incorporated into the polymer matrix at varying concentrations. The samples were then characterized using X-ray diffraction, scanning electron microscope, infrared spectroscopy, and UV-Vis optical measurements. The antibacterial activity of the prepared composites was evaluated using a diffusion assay on agar medium against Staphylococcus aureus and Escherichia coli. The results showed that composites with low bismuth ferrite loading ratios did not exhibit any significant inhibitory activity, even with increasing sample mass. A clear antibacterial effect was observed only at a critical loading ratio of 0.09 g/ml, with the diameter of the inhibition zones increasing with increasing fiber mass. This behavior is attributed to improved availability of nanoparticles on the fiber surface and an increased density of active sites capable of interacting with bacterial cells. These results confirm that precise control of the nanoparticle loading ratio is a crucial factor in activating the biological performance of bismuth ferrite/PAAm composites, highlighting their potential as functional fibrous materials for advanced bioapplications. Electrospun PAAm:Bi₂Fe₄O₉ Nanofiber Membranes Iron-Mediated Charge Dynamics Antibacterial Efficacy Reactive Oxygen Species Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. 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