Electrospinning of hydroxypropyl chitosan nanofibers for bone regeneration application

Electrospinning of hydroxypropyl chitosan nanofibers for bone regeneration application | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Electrospinning of hydroxypropyl chitosan nanofibers for bone regeneration application junmei wang, jie xiong, guozhao li, ziliang zhou, yanyan yang, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-9074906/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 11 You are reading this latest preprint version Abstract To futher explore the effect of electrospinning nanofiber mats synthesized using hydroxypropyl chitosan in conjugation with poly (vinyl alcohol) on the osteoconducive in MC3T3 cell, we tested different ratios of hydroxypropyl chitosan (HPCH) embedded with poly (vinyl alcohol) to electrospin the nanofiber mats, the mechanical and physical properties of nanofiber mats were verified by using SEM, FT-IR and conventional methods. Then the capacity of the electrospinning mats on osteoconducive in MC3T3 cell were evaluated by Alizarin Red stain, ALP stain and relative expression level of relevant genes. The results testified that the nanofiber mats embeded hydroxypropyl chitosan (50%)with poly (vinyl alcohol)(50%) can induce osteoblast differentiation apparently in MC3T3 cell. Biological sciences/Biotechnology Biological sciences/Cell biology Physical sciences/Materials science electrospining hydroxypropyl chitosan Osteoblast MC3T3 cells Full Text Additional Declarations No competing interests reported. Supplementary Files supplementaryfile.pdf Cite Share Download PDF Status: Under Review Version 1 posted Reviews received at journal 18 May, 2026 Reviews received at journal 17 May, 2026 Reviewers agreed at journal 04 May, 2026 Reviewers agreed at journal 03 May, 2026 Reviewers agreed at journal 30 Apr, 2026 Reviewers agreed at journal 30 Apr, 2026 Reviewers invited by journal 29 Apr, 2026 Editor assigned by journal 29 Apr, 2026 Editor invited by journal 28 Apr, 2026 Submission checks completed at journal 07 Apr, 2026 First submitted to journal 07 Apr, 2026 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-9074906","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":634457646,"identity":"c1a57010-8819-4f90-9bb8-c99bb3ed4a13","order_by":0,"name":"junmei wang","email":"","orcid":"","institution":"Guangzhou Medical University","correspondingAuthor":false,"prefix":"","firstName":"junmei","middleName":"","lastName":"wang","suffix":""},{"id":634457649,"identity":"62b6a8c3-efc3-45d2-af2b-de08b00d3055","order_by":1,"name":"jie 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hydroxypropyl chitosan, Osteoblast, MC3T3 cells","lastPublishedDoi":"10.21203/rs.3.rs-9074906/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-9074906/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eTo futher explore the effect of electrospinning nanofiber mats synthesized using hydroxypropyl chitosan in conjugation with poly (vinyl alcohol) on the osteoconducive in MC3T3 cell, we tested different ratios of hydroxypropyl chitosan (HPCH) embedded with poly (vinyl alcohol) to electrospin the nanofiber mats, the mechanical and physical properties of nanofiber mats were verified by using SEM, FT-IR and conventional methods. 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