Screening, dose-response, and management of Echinochloa crus-galli biotypes to bispyribac- sodium: Implications for histophysiological characteristics, agronomic attributes, and amylose in irrigated rice

Screening, dose-response, and management of Echinochloa crus-galli biotypes to bispyribac- sodium: Implications for histophysiological characteristics, agronomic attributes, and amylose in irrigated rice | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Screening, dose-response, and management of Echinochloa crus-galli biotypes to bispyribac- sodium: Implications for histophysiological characteristics, agronomic attributes, and amylose in irrigated rice Koko Tampubolon, Alridiwirsah Alridiwirsah, Sanggam Dera Rosa Tampubolon, and 5 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-9022509/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract The presence of barnyardgrass negatively affects productivity in irrigated rice. Although farmers predominantly used the bispyribac-sodium herbicide, prolonged application has inadvertently led to resistance, making it necessary to assess resistance levels for guided control strategies that combine herbicides with different modes of action (MoA). Therefore, this study aimed to (1) determine the resistance index of barnyardgrass to bispyribac-sodium herbicide, (2) identify active ingredients (a.i.) in mixed herbicides for controlling resistant barnyardgrass biotype and to evaluate the effects on histophysiological, agronomic attributes, and amylose content in irrigated rice. The experiment started by collecting barnyardgrass seeds from five accessions in irrigated rice fields, and then sprayed with the bispyribac-sodium at the recommended dose (103 g a.i.ha − 1 ) from April to July 2023. The seeds in the resistant category were continued in dose-response testing at 0, 1/16, 1/8, 1/4, 1/2, 1, 2, 4, 8, and 16-fold the recommended dose from August to November 2023. Biotype progenies from low, labeled, and high-doses were further evaluated for resistance management using cyhalofop-butyl+penoxsulam, florpyrauxifen-benzyl+cyhalofop-butyl, fenoxaprop-P-ethyl+ethoxysulfuron, and bentazone+MCPA at the recommended dose from July to December 2024. Parameters were observed and analyzed by ANOVA and DMRT. The results showed that the SB3-accession was 9.82-fold more resistant to the bispyribac-sodium herbicide compared to the susceptible. The fenoxaprop-P-ethyl+ethoxysulfuron demonstrated 100% effectiveness in controlling barnyardgrass of resistant biotype progenies, thereby increasing irrigated rice productivity and amylose content were 27.01 and 31.50%. In conclusion, this study achieved effective management of resistant-biotype by combination of herbicides with different MoA. barnyardgrass bispyribac-sodium herbicide mixture identification resistance Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-9022509","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":608171325,"identity":"c914db20-984f-4f03-b707-e21f13ff07e7","order_by":0,"name":"Koko Tampubolon","email":"","orcid":"","institution":"National Research and Innovation Agency","correspondingAuthor":false,"prefix":"","firstName":"Koko","middleName":"","lastName":"Tampubolon","suffix":""},{"id":608171326,"identity":"23aff951-f071-4817-826e-e18074b2e2b6","order_by":1,"name":"Alridiwirsah Alridiwirsah","email":"","orcid":"","institution":"Universitas Muhammadiyah Sumatera 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Although farmers predominantly used the bispyribac-sodium herbicide, prolonged application has inadvertently led to resistance, making it necessary to assess resistance levels for guided control strategies that combine herbicides with different modes of action (MoA). Therefore, this study aimed to (1) determine the resistance index of barnyardgrass to bispyribac-sodium herbicide, (2) identify active ingredients (a.i.) in mixed herbicides for controlling resistant barnyardgrass biotype and to evaluate the effects on histophysiological, agronomic attributes, and amylose content in irrigated rice. The experiment started by collecting barnyardgrass seeds from five accessions in irrigated rice fields, and then sprayed with the bispyribac-sodium at the recommended dose (103 g a.i.ha\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e) from April to July 2023. The seeds in the resistant category were continued in dose-response testing at 0, 1/16, 1/8, 1/4, 1/2, 1, 2, 4, 8, and 16-fold the recommended dose from August to November 2023. Biotype progenies from low, labeled, and high-doses were further evaluated for resistance management using cyhalofop-butyl+penoxsulam, florpyrauxifen-benzyl+cyhalofop-butyl, fenoxaprop-P-ethyl+ethoxysulfuron, and bentazone+MCPA at the recommended dose from July to December 2024. Parameters were observed and analyzed by ANOVA and DMRT. The results showed that the SB3-accession was 9.82-fold more resistant to the bispyribac-sodium herbicide compared to the susceptible. The fenoxaprop-P-ethyl+ethoxysulfuron demonstrated 100% effectiveness in controlling barnyardgrass of resistant biotype progenies, thereby increasing irrigated rice productivity and amylose content were 27.01 and 31.50%. 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