Protective effect of Allium Scorodoprasum L. ethanolic extract in cyclophosphamide- induced hepatotoxicity model in rats Short Title: Allium Scorodoprasum L. extract can be protective in hepatotoxicity

Purpose: Cyclophosphamide(CP) is a chemotherapeutic agent and immunosuppressant drug however it damages the liver. The aim of this study is to investigate the protective effect of ethanolic extract of Allium scorodoprasum(ASE) on CP-induced liver injury. Methods: : A total of 28 Wistar albino rats were randomly divided into four groups (n=7/group): Healthy, CP(200 mg/kg), CP(200 mg/kg)+ASE(100 mg/kg), CP(200 mg/kg)+ASE(200mg/kg). ASE was administered for 14 days, and the rats were euthanized 24 hours after CP administration. Results: : CP Treatment leads to an increase in serum levels ALT, AST, LDH, total cholesterol, triglycerides, LDL, VLDL and liver levels MDA, TNF, IL-1β and IL-6, while HDL levels decrease, statistically. Treatment with CP caused liver necrosis and postnecrotic cell infiltration; however, the pathologic changes were prevented by ASE. Immunohistochemically, 8-OhDG, 4-HNE, and diTYR levels increased in the treatment with CP, whereas they decreased in the groups treated with ASE. All these changes were dose dependent in the ASE-treated groups. Conclusion: The treatment with CP caused liver damage due to oxidative stress and inflammation. ASE regulated the damage at high doses because it has potent antioxidant and anti-inflammatory ingredients. In future studies, it may be more beneficial to use ASE in higher doses or for a longer period of time.