Gallium-68 labelled aptamer assay for Staphylococcus aureus detection

Gallium-68 labelled aptamer assay for Staphylococcus aureus detection | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Gallium-68 labelled aptamer assay for Staphylococcus aureus detection Saharnaz Bargh, Ceren Ozkul Kocak, Veli C. Ozalp, Suna Erdogan This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4977986/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Staphylococcus aureus infection can result in a serious inflammatory condition of bone tissue, osteomyelitis with significant challenges in diagnostics. The cell surface components of the bacteria can serve as binding sites for the precise and targeted delivery of diagnostic imaging agents. We created a novel infection imaging substance using a Gallium 68 (Ga-68) labelled aptamer that specifically targets S. aureus bacteria. The targeting aptamer was selected by cell-SELEX (Systematic Evolution of Ligands by Exponential Enrichment) procedure with affinity constant at 2260 ± 634 CFU/mL. the linear detection range was between 250 and 2 x 10 4 CFU/mL. the limit of detection for s. aureus was 171 CFU/mL. The aptamer was conjugated to Ga-68 by DOTA procedure. Radioactive assays in vitro were specific for S. aureus between 10 3 and 2x10 4 CFU/mL. The encouraging outcomes indicate that this aptamer probe holds substantial promise for future in vivo experimentation, which might aid in clarifying its suitability for diagnosing S. aureus. Aptamers SELEX Staphylococcus aureus Radiolabelling Gallium Full Text Additional Declarations Table 1 is available in the Supplementary Files section. Supplementary Files Table1.tif Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4977986","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":357616412,"identity":"28db47fe-9740-45d7-a127-94f419ceebd9","order_by":0,"name":"Saharnaz Bargh","email":"","orcid":"","institution":"Hacettepe University","correspondingAuthor":false,"prefix":"","firstName":"Saharnaz","middleName":"","lastName":"Bargh","suffix":""},{"id":357616413,"identity":"9f232373-6c22-476a-acbd-b5ded273f739","order_by":1,"name":"Ceren Ozkul Kocak","email":"","orcid":"","institution":"Hacettepe University","correspondingAuthor":false,"prefix":"","firstName":"Ceren","middleName":"Ozkul","lastName":"Kocak","suffix":""},{"id":357616414,"identity":"ef24b34b-72aa-43ed-99b1-875dde1816fc","order_by":2,"name":"Veli C. 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