Multiple Flagellin Proteins Have Distinct and Synergistic Roles inAgrobacterium tumefaciensMotility

Rotary flagella propel bacteria through liquid and across semi-solid environments. Flagella are composed of the basal body that constitutes the motor for rotation, the curved hook that connects to the basal body, and the flagellar filament that propels the cell. Flagellar filaments can be comprised of a single flagellin protein such as in Escherichia coli or with multiple flagellins such is in Agrobacterium tumefaciens . The four distinct flagellins FlaA, FlaB, FlaC and FlaD produced by wild type A. tumefaciens , are not redundant in function, but have specific properties. FlaA and FlaB are much more abundant than FlaC and FlaD and are readily observable in mature flagellar filaments, when either FlaA or FlaB is fluorescently labeled. Cells having FlaA with any one of the other three flagellins can generate functional filaments and thus are motile, but FlaA alone cannot constitute a functional filament. In flaA mutants that manifest swimming deficiencies, there are multiple ways by which these mutations can be phenotypically suppressed. These suppressor mutations primarily occur within or upstream of the flaB flagellin gene or in the transcriptional factor sciP regulating flagellar expression. The helical conformation of the flagellar filament appears to require a key asparagine residue present in FlaA and absent in other flagellins. However, FlaB can be spontaneously mutated to render helical flagella in absence of FlaA, reflecting their overall similarity and perhaps the subtle differences in the specific functions they have evolved to fulfill. Importance Flagellins are abundant bacterial proteins comprising the flagellar filaments that propel bacterial movement. Several members of the Alphaproteobacterial group express multiple flagellins, in contrast to model systems such as Escherichia coli that has only one flagellin protein. The plant pathogen Agrobacterium tumefaciens has four flagellins, the abundant and readily detected FlaA and FlaB, and lower levels of FlaC and FlaD. Mutational analysis reveals that FlaA requires at least one of the other flagellins to function - flaA mutants produce non-helical flagella and cannot swim efficiently. Suppressor mutations can rescue this swimming defect through mutations in the remaining flagellins, including structural changes imparting flagellar helical shape, and putative regulators. Our findings shed light on how multiple flagellins contribute to motility.