Methylobrevis albus sp. nov., isolated from freshwater lake sediment

Abstract An aerobic, Gram-stain-negative, rod-shaped bacterium with flagellum, designated L22T, was isolated from freshwater of Hulun Lake, Inner Mongolia, China. The organism was found to grow optimally at 30 ℃ in medium containing 0-0.75%(w/v) NaCl at pH 7.5. The major fatty acid identified was summed feature 8 (C16:1ω7c). The dominant polar lipids were phosphomonoester, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The main ubiquinone was Q-10. The G + C content of genomic DNA was 69.8 mol%. The 16S rRNA gene sequences indicated that strain L22T was affiliated with the genus Methylobrevis within the family Pleomorphomonadaceae, being most closely related to M. pamukkalensis JCM 30229T with 95.9% sequences similarity. Based on taxonomic results in this study, we proposed that strain L22T was placed into a novel species in the genus Methylobrevis of the family Pleomorphomonadaceae, for which the name Methylobrevis albus sp. nov. is proposed. The type strain is L22T (= KCTC 72858T = MCCC 1H00432T).