Whole Genome Sequence of Biosurfactant Producing Bacillus Tequilensis

Abstract Background: Bioremediation is crucial for recuperate polluted water and soil. By expanding the surface area of substrates, biosurfactants play a vital role in bioremediation. Bioremediation is crucial for recuperate polluted water and soil. By expanding the surface area of substrates, biosurfactants play a vital role in bioremediation. Biosurfactant producing microbes release certain biosurfactant compounds, which are promoted for oil spill remediation. In the present investigation, a biosurfactant producing bacterium Bacillus tequilensis was isolated from Chilika lake, Odisha, India (latitude and longitude: 19.8450 N 85.4788 E). Whole Genome Sequencing (WGS) of Bacillus tequilensis was carried out using Illumina NextSeq 500. Results: The whole genome sequence is 4.47 MB consisting of 4,478,749 base pairs forming a circular chromosome with 528 scaffolds, 4492 protein-encoding genes(ORFs), 81 tRNA genes, and 114 ribosomal RNA transcription units. The total number of raw reads was 4209415, and processed reads were 4058238 with predicted genes of 4492. The whole-genome obtained from the present investigation was used for genome annotation, variant calling, variant annotation and comparative genome analysis with other existing Bacillus species. In this study, a pathway was constructed which describe the biosurfactant metabolism of Bacillus tequilensis. The study identified genes such as SrfAD, SrfAC, SrfAA, SrfAB which are involved in biosurfactant synthesis. Conclusion: The sequence of the genes SrfAD, SrfAC, SrfAA, SrfAB was deposited in Genbank database with accession MUG02427.1, MUG02428.1, MUG02429.1, MUG03515.1 respectively. The whole-genome sequence was submitted to Genbank with an accession RMVO00000000 and the raw reads can be obtained from SRA, NCBI repository using accession: SRX5023292.