Adsorption characteristics of Cd2+ by Agaricus bisporus (Large) mycelia, extracellular polymer substances and mycelial cell wall

Adsorption characteristics of Cd2+ by Agaricus bisporus (Large) mycelia, extracellular polymer substances and mycelial cell wall | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Adsorption characteristics of Cd 2+ by Agaricus bisporus (Large) mycelia, extracellular polymer substances and mycelial cell wall He Haiyan, Zhang Dan This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-9280763/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Cadmium (Cd) is a highly toxic heavy metal and a major soil contaminant in China. The development of efficient remediation technologies remains a key research focus. Macrofungi-based remediation represents a promising strategy. This study investigated the Cd 2+ adsorption characteristics of Agaricus bisporus (Large) mycelia, extracellular polymeric substances (EPS), and cell wall. Results showed that Cd 2+ stress induced a hormetic response in EPS production and aitered EPS composition. All components displayed favorable Cd 2+ adsorption properties, with EPS exhibited an outstanding maximum adsorption capacity of 583.07 mg/g dry weight—tenfold higher than those of mycelia and cell wall. Adsorption kinetics of all components fitted the pseudo-second-order kinetic equation. Cd 2+ adsorption by EPS conformed to the Langmuir isotherm model, whereas mycelia and cell wall followed the Freundlich isotherm model. In conclusion, the high Cd 2+ binding capacities of EPS and cell walls constitute a potential key mechanism enabling A. bisporus to resist extracellular Cd stress and alleviate Cd toxicity. Biological sciences/Biotechnology Earth and environmental sciences/Environmental sciences Cd pollution adsorption characteristics extracellular polymeric substances Agaricus bisporus (Large) Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-9280763","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":622431009,"identity":"210630a7-83c4-4348-a840-6241e8dadcb0","order_by":0,"name":"He Haiyan","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAw0lEQVRIiWNgGAWjYBACAwYeBoYPDGxgjgTRWhhnMLBJkKaFmQeqmjgt5uy9x6Rtd/DV8TcwH7zNw2CXR1CLZc+5NOncM2wSEgfYkq15GJKLCTvsRo6ZdG4bmwTQhWbSPAwHEhsIarn/xkzaEqyF/xuRWm4ADWeE2MJGpJYzOcaWvW1skjMOsxlbzjFIJkLL8TOGN362HePnb29+eONNhR1hLUDAAoyOYwwMzGATiFAPBMwfGBhqiFM6CkbBKBgFIxMAAJrJMdPrddURAAAAAElFTkSuQmCC","orcid":"","institution":"Neijiang Normal University","correspondingAuthor":true,"prefix":"","firstName":"He","middleName":"","lastName":"Haiyan","suffix":""},{"id":622431010,"identity":"715c82dc-f226-4a3a-ba60-e91c345cde79","order_by":1,"name":"Zhang Dan","email":"","orcid":"","institution":"Chinese Academy of Sciences","correspondingAuthor":false,"prefix":"","firstName":"Zhang","middleName":"","lastName":"Dan","suffix":""}],"badges":[],"createdAt":"2026-03-31 13:53:14","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-9280763/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-9280763/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":108807003,"identity":"b74b3dc2-0843-489e-ba7f-2c236c5927ba","added_by":"auto","created_at":"2026-05-08 15:29:53","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":418091,"visible":true,"origin":"","legend":"","description":"","filename":"Manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-9280763/v1_covered_b4e65ab2-0f87-470e-9a2c-d721a37c0446.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"\u003cp\u003eAdsorption characteristics of Cd\u003csup\u003e2+\u003c/sup\u003e by Agaricus bisporus (Large) mycelia, extracellular polymer substances and mycelial cell wall\u003c/p\u003e","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Cd pollution, adsorption characteristics, extracellular polymeric substances, Agaricus bisporus (Large)","lastPublishedDoi":"10.21203/rs.3.rs-9280763/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-9280763/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eCadmium (Cd) is a highly toxic heavy metal and a major soil contaminant in China. The development of efficient remediation technologies remains a key research focus. Macrofungi-based remediation represents a promising strategy. This study investigated the Cd\u003csup\u003e2+\u003c/sup\u003e adsorption characteristics of \u003cem\u003eAgaricus bisporus\u003c/em\u003e (Large) mycelia, extracellular polymeric substances (EPS), and cell wall. Results showed that Cd\u003csup\u003e2+\u003c/sup\u003e stress induced a hormetic response in EPS production and aitered EPS composition. All components displayed favorable Cd\u003csup\u003e2+\u003c/sup\u003e adsorption properties, with EPS exhibited an outstanding maximum adsorption capacity of 583.07 mg/g dry weight\u0026mdash;tenfold higher than those of mycelia and cell wall. Adsorption kinetics of all components fitted the pseudo-second-order kinetic equation. Cd\u003csup\u003e2+\u003c/sup\u003e adsorption by EPS conformed to the Langmuir isotherm model, whereas mycelia and cell wall followed the Freundlich isotherm model. 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