The New Probiotic Lactobacillus Plantarum Strain Isolated from Traditional Dairy Showed The Synergistic Effect on Aflatoxin B1 Detoxification Along with Nanochitosan Particles

Abstract Background: Reduction of aflatoxin toxins in food products is of great importance. Therefore, in the present study, the effects of new Lactobacillus plantarum strain isolated from dairy products as well as chitosan nanoparticles were studied on reducing of aflatoxin B1 (AFB1) toxicity in vitro.Methods: After collection and preparation yogurt, cheese, milk and whey products, lactic acid bacteria (LABs) were isolated and identified using biochemical and molecular methods. To measure probiotic activity, pH, bile and salt tolerance tests were used. Then, the antimicrobial activity of LABs against gastrointestinal pathogens was studied. Next, the cheese-separated strain (C1) was selected as the superior strain and antibiotic susceptibility testing was performed. Then, the effect of C1 isolate and chitosan nanoparticles on reducing aflatoxin B1 (AFB1) in the medium was studied by measuring AFB1 using the enzyme-linked immunosorbent assay (ELISA) and high performance liquid chromatography (HPLC).Results: The results of biochemical evaluations indicated the separation of different strains of Lactobacillus plantarum. Antimicrobial activity test showed extensive antimicrobial activity of C1 isolate. The results showed that this strain has good probiotic activities. This strain was shown to be resistant to the antibiotics erythromycin, fusidic acid, gentamicin, kanamycin, nalidixic acid, neomycin, ofloxacin and vancomycin. C1 strain together with chitosan nanoparticles had the ability to reduce AFB1 in the medium and when both were used simultaneously, and synergistic effect in reducing AFB1 from the medium was seen.Conclusion: In general, it was concluded that the new C1 L. plantarum strains together with chitosan nanoparticles could have synergistic effects in reducing AFB1 toxin in food products.