Photosynthesis promotes pre-protein processing and progression of chloroplast biogenesis during early plant development

Photosynthesis promotes pre-protein processing and progression of chloroplast biogenesis during early plant development | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Photosynthesis promotes pre-protein processing and progression of chloroplast biogenesis during early plant development Felix Kessler, Joy Collombat, Manfredo Quadroni, Véronique Douet, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4205963/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 22 Jan, 2025 Read the published version in Communications Biology → Version 1 posted You are reading this latest preprint version Abstract Photosynthetic activity is established during chloroplast biogenesis, but whether photosynthesis itself is required for chloroplast biogenesis has not been clarified. In this study we use 680nm red light to overexcite Photosystem II and disrupt photosynthesis in two conditional mutants (var2 and abc1k1) which reversibly arrests chloroplast biogenesis. During their biogenesis, chloroplasts import most proteins associated with photosynthesis. Some of these must be transported further across the thylakoid membrane into the thylakoid lumen by the evolutionarily conserved SEC (Secretory) and TAT (Twin Arginine Translocation) pathways, energized by ATP and the trans-thylakoid proton gradient, respectively. Most luminal thylakoid proteins are synthesized in the cytoplasm with bi-partite, cleavable targeting sequences (first for the chloroplast envelope, second for the thylakoid membrane). Two-stage cleavage of these peptides is a critical step of chloroplast biogenesis. Linked to the photosynthetic defect in var2 and abc1k1 under red light, six essential proteins at the thylakoid lumen side of Photosystems I and II were incompletely cleaved and intermediate forms accumulated to high levels in the mutants. The results show that the processing of a specific module of Photosystem-associated proteins and concomitantly progression of chloroplast biogenesis depend on active photosynthesis at the earliest stages of seedling development. Biological sciences/Plant sciences/Photosynthesis Biological sciences/Cell biology Full Text Additional Declarations There is NO Competing Interest. Supplementary Tables are not available with this version. Cite Share Download PDF Status: Published Journal Publication published 22 Jan, 2025 Read the published version in Communications Biology → Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4205963","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":291316102,"identity":"2f1c4d22-61bc-4d06-91f8-140de0edf5bd","order_by":0,"name":"Felix 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Linked to the photosynthetic defect in var2 and abc1k1 under red light, six essential proteins at the thylakoid lumen side of Photosystems I and II were incompletely cleaved and intermediate forms accumulated to high levels in the mutants. 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