Assessment of Platelet Indices, Beta-Thromboglobulin and Platelet Factor Iv in Platelet Concentrate at University of Ilorin Teaching Hospital, Nigeria

Assessment of Platelet Indices, Beta-Thromboglobulin and Platelet Factor Iv in Platelet Concentrate at University of Ilorin Teaching Hospital, Nigeria | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Assessment of Platelet Indices, Beta-Thromboglobulin and Platelet Factor Iv in Platelet Concentrate at University of Ilorin Teaching Hospital, Nigeria Luqman Olayinka Olatunbosun, Musa Adewale Muhibi, Olusegun Taiwo Oke, and 9 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7016057/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 26 Nov, 2025 Read the published version in Scientific Reports → Version 1 posted 10 You are reading this latest preprint version Abstract OBJECTIVES: Platelet concentrate transfusion remain a major therapeutic care for patient with hematological disorders. However, optimal therapeutic effect has been a major challenge. Blood donor characteristics and processing procedure remain major areas of research. Hence, this research assessed the platelet functional parameters, beta-thromboglobulin and Platelets factor IV in donors and platelet concentrate. MATERIALS AND METHODS: The study was a cross-sectional descriptive study among prospective blood donors for platelet concentrates. Seventy-one prospective blood donors were recruited for this study with mean age of 34.8. Whole blood and aliquot from concentrates were collected for platelet functional parameters using Semi-automated Haematology analyzer, pH using potentiometric method while Beta-thromboglobulin and Platelet factor IV using ELISA-based assays. RESULTS: There was significant increase (P-value ˂0.05) in platelet count, plateletcrit (PCT), β-thromboglobulin (BTG) and platelet large cell concentration (PLCC) in the first 5 hours of harvest and running through 5 days of storage pre-transfusion. Conversely, there was significant decrease in Mean platelet volume (MPV), platelet distribution width coefficient of variation (PDW-CV) and Platelets factor IV (PF4). Interestingly, BTG levels exhibited a non-significant increase, rising from 114.40 ± 28.59 at 2 hours to 146.58 ± 32.83 at 5 hours, indicating a slight but steady increase in platelet activation markers as storage time progresses. CONCLUSION: Platelet concentrate is of high quality when transfused within 2 hours of harvest and not more than 5 hours post-harvest in order to optimize the patient outcome. Plateletcrit (PCT), β-thromboglobulin (BTG) and platelet large cell concentration (PLCC), mean platelet volume (MPV), platelet distribution width coefficient of variation (PDW-CV) and Platelets factor IV (PF4) remain functional parameters in determining functionality of platelets as established by this study. Health sciences/Biomarkers Health sciences/Diseases Health sciences/Medical research Platelets Beta-thromboglobulin Factor IV Platelet Concentrates Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Published Journal Publication published 26 Nov, 2025 Read the published version in Scientific Reports → Version 1 posted Editorial decision: Revision requested 30 Jul, 2025 Reviews received at journal 29 Jul, 2025 Reviews received at journal 26 Jul, 2025 Reviewers agreed at journal 24 Jul, 2025 Reviewers agreed at journal 22 Jul, 2025 Reviewers invited by journal 09 Jul, 2025 Editor assigned by journal 09 Jul, 2025 Editor invited by journal 09 Jul, 2025 Submission checks completed at journal 07 Jul, 2025 First submitted to journal 07 Jul, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7016057","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":483240679,"identity":"bb77f1d1-88f0-48ad-b4dd-ed1ef8082414","order_by":0,"name":"Luqman Olayinka 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Nigeria\u003c/p\u003e","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"scientific-reports","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scirep","sideBox":"Learn more about [Scientific Reports](http://www.nature.com/srep/)","snPcode":"","submissionUrl":"","title":"Scientific Reports","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Scientific Reports","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"Platelets, Beta-thromboglobulin, Factor IV, Platelet Concentrates","lastPublishedDoi":"10.21203/rs.3.rs-7016057/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7016057/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eOBJECTIVES: Platelet concentrate transfusion remain a major therapeutic care for patient with hematological disorders. However, optimal therapeutic effect has been a major challenge. Blood donor characteristics and processing procedure remain major areas of research. Hence, this research assessed the platelet functional parameters, beta-thromboglobulin and Platelets factor IV in donors and platelet concentrate.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eMATERIALS AND METHODS: The study was a cross-sectional descriptive study among prospective blood donors for platelet concentrates. Seventy-one prospective blood donors were recruited for this study with mean age of 34.8. Whole blood and aliquot from concentrates were collected for platelet functional parameters using Semi-automated Haematology analyzer, pH using potentiometric method while Beta-thromboglobulin and Platelet factor IV using ELISA-based assays.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eRESULTS: There was significant increase (P-value ˂0.05) in platelet count, plateletcrit (PCT), β-thromboglobulin (BTG) and platelet large cell concentration (PLCC) in the first 5 hours of harvest and running through 5 days of storage pre-transfusion. Conversely, there was significant decrease in Mean platelet volume (MPV), platelet distribution width coefficient of variation (PDW-CV) and Platelets factor IV (PF4). Interestingly, BTG levels exhibited a non-significant increase, rising from 114.40 ± 28.59 at 2 hours to 146.58 ± 32.83 at 5 hours, indicating a slight but steady increase in platelet activation markers as storage time progresses.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eCONCLUSION: Platelet concentrate is of high quality when transfused within 2 hours of harvest and not more than 5 hours post-harvest in order to optimize the patient outcome. Plateletcrit (PCT), β-thromboglobulin (BTG) and platelet large cell concentration (PLCC), mean platelet volume (MPV), platelet distribution width coefficient of variation (PDW-CV) and Platelets factor IV (PF4) remain functional parameters in determining functionality of platelets as established by this study.\u003c/p\u003e","manuscriptTitle":"Assessment of Platelet Indices, Beta-Thromboglobulin and Platelet Factor Iv in Platelet Concentrate at University of Ilorin Teaching Hospital, Nigeria","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-07-11 18:20:35","doi":"10.21203/rs.3.rs-7016057/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2025-07-30T11:49:29+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-07-29T17:29:57+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-07-26T04:00:51+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"173473230607820507462422773827327006656","date":"2025-07-24T07:07:43+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"158028336441990236635299132136576449618","date":"2025-07-22T14:38:16+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2025-07-09T13:14:16+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2025-07-09T12:01:32+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"","date":"2025-07-09T10:47:46+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2025-07-07T09:51:52+00:00","index":"","fulltext":""},{"type":"submitted","content":"Scientific Reports","date":"2025-07-07T09:50:37+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"scientific-reports","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scirep","sideBox":"Learn more about [Scientific Reports](http://www.nature.com/srep/)","snPcode":"","submissionUrl":"","title":"Scientific Reports","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Scientific Reports","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"333a2255-6145-42e7-8b3c-3249cd010555","owner":[],"postedDate":"July 11th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[{"id":51415674,"name":"Health sciences/Biomarkers"},{"id":51415675,"name":"Health sciences/Diseases"},{"id":51415676,"name":"Health sciences/Medical research"}],"tags":[],"updatedAt":"2025-12-01T16:01:58+00:00","versionOfRecord":{"articleIdentity":"rs-7016057","link":"https://doi.org/10.1038/s41598-025-26247-y","journal":{"identity":"scientific-reports","isVorOnly":false,"title":"Scientific Reports"},"publishedOn":"2025-11-26 15:57:09","publishedOnDateReadable":"November 26th, 2025"},"versionCreatedAt":"2025-07-11 18:20:35","video":"","vorDoi":"10.1038/s41598-025-26247-y","vorDoiUrl":"https://doi.org/10.1038/s41598-025-26247-y","workflowStages":[]},"version":"v1","identity":"rs-7016057","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-7016057","identity":"rs-7016057","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}