Based on multi omics analysis of gut microbiota characteristics in patients with acute pancreatitis and exploring the possible mechanism of its exacerbation trend from the level of microbiota and its metabolites

Based on multi omics analysis of gut microbiota characteristics in patients with acute pancreatitis and exploring the possible mechanism of its exacerbation trend from the level of microbiota and its metabolites | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Based on multi omics analysis of gut microbiota characteristics in patients with acute pancreatitis and exploring the possible mechanism of its exacerbation trend from the level of microbiota and its metabolites Zhenjiang Wang, Mingyi Guo, Sen Yang, Yuping Chen, Jianbin Cheng, and 8 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3919359/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 30 Oct, 2024 Read the published version in Gut Pathogens → Version 1 posted 8 You are reading this latest preprint version Abstract Specific intestinal metabolites are closely associated with the classification, severity, and necrosis of acute pancreatitis (AP), providing novel insights for in-depth clinical investigation of AP. In this study, the gut microbiota and metabolites of 49 AP patients at different treatment stages and severities were analyzed using 16s RNA sequencing and untargeted metabolomics to investigate the trends observed in severe AP based on gut microbiota composition and metabolome profiles. The findings revealed an imbalance in intestinal flora homeostasis among AP patients characterized by a decrease in probiotics and an increase in opportunistic pathogens, leading to damage to the intestinal mucosal barrier through reduced short-chain fatty acid (SCFA) secretion and disruption of the intestinal epithelium. This dysbiosis further influenced energy metabolism, anti-inflammatory responses, and immune regulation while also highlighting significant differences in energy metabolic pathways. These results suggest that differential composition of intestinal flora along with alterations in intestinal metabolites and metabolic pathways contribute to compromised integrity of the intestinal mucosal barrier as well as disturbances in energy metabolism within severe AP patients. Acute pancreatitis Gastrointestinal microbiota Metabolites/metabolic pathways Severe trend Multiple omics Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Published Journal Publication published 30 Oct, 2024 Read the published version in Gut Pathogens → Version 1 posted Editorial decision: Revision requested 19 Mar, 2024 Reviewers agreed at journal 13 Mar, 2024 Reviews received at journal 13 Mar, 2024 Reviewers agreed at journal 20 Feb, 2024 Reviewers invited by journal 14 Feb, 2024 Editor assigned by journal 04 Feb, 2024 Submission checks completed at journal 04 Feb, 2024 First submitted to journal 01 Feb, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3919359","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":271045021,"identity":"96e7118e-b277-4fad-983c-4308aeabb7b8","order_by":0,"name":"Zhenjiang Wang","email":"","orcid":"","institution":"Zhuhai People's Hospital","correspondingAuthor":false,"prefix":"","firstName":"Zhenjiang","middleName":"","lastName":"Wang","suffix":""},{"id":271045022,"identity":"433512a3-9952-482b-9ad2-bcbeacb7502c","order_by":1,"name":"Mingyi Guo","email":"","orcid":"","institution":"Zhuhai People's Hospital","correspondingAuthor":false,"prefix":"","firstName":"Mingyi","middleName":"","lastName":"Guo","suffix":""},{"id":271045023,"identity":"0062b802-d8a1-474e-a5ee-160747065b7d","order_by":2,"name":"Sen Yang","email":"","orcid":"","institution":"Zhuhai People's Hospital","correspondingAuthor":false,"prefix":"","firstName":"Sen","middleName":"","lastName":"Yang","suffix":""},{"id":271045024,"identity":"61bb6d22-db86-447d-805e-8e94095e4da0","order_by":3,"name":"Yuping Chen","email":"","orcid":"","institution":"Zhuhai People's Hospital","correspondingAuthor":false,"prefix":"","firstName":"Yuping","middleName":"","lastName":"Chen","suffix":""},{"id":271045025,"identity":"5f64f4a4-aef1-4951-b0e0-2047aa084aa4","order_by":4,"name":"Jianbin Cheng","email":"","orcid":"","institution":"Zhuhai People's Hospital","correspondingAuthor":false,"prefix":"","firstName":"Jianbin","middleName":"","lastName":"Cheng","suffix":""},{"id":271045026,"identity":"f542519b-3f43-49c2-aaf5-30d4f8d188f5","order_by":5,"name":"Zaiwei Huang","email":"","orcid":"","institution":"Zhuhai People's Hospital","correspondingAuthor":false,"prefix":"","firstName":"Zaiwei","middleName":"","lastName":"Huang","suffix":""},{"id":271045027,"identity":"1b5bc533-f8cc-4620-99dd-b8a1cdf9b76f","order_by":6,"name":"Tongxu Wang","email":"","orcid":"","institution":"Zhuhai People's Hospital","correspondingAuthor":false,"prefix":"","firstName":"Tongxu","middleName":"","lastName":"Wang","suffix":""},{"id":271045028,"identity":"fc6aa5e4-31a6-4326-a102-569bdd9d14ec","order_by":7,"name":"Weifan Zhang","email":"","orcid":"","institution":"Milin People's Hospital","correspondingAuthor":false,"prefix":"","firstName":"Weifan","middleName":"","lastName":"Zhang","suffix":""},{"id":271045029,"identity":"922f2069-11a6-4922-b29e-db1967f065dc","order_by":8,"name":"Lamu Gama","email":"","orcid":"","institution":"Milin People's Hospital","correspondingAuthor":false,"prefix":"","firstName":"Lamu","middleName":"","lastName":"Gama","suffix":""},{"id":271045030,"identity":"c7548350-af39-4c64-b2b8-fa7dd0a431df","order_by":9,"name":"Cuomu Zhaxi","email":"","orcid":"","institution":"Milin People's Hospital","correspondingAuthor":false,"prefix":"","firstName":"Cuomu","middleName":"","lastName":"Zhaxi","suffix":""},{"id":271045031,"identity":"262ff364-709b-4256-8627-36088dc30c51","order_by":10,"name":"Xiaobei Luo","email":"","orcid":"","institution":"Nanfang Hospital","correspondingAuthor":false,"prefix":"","firstName":"Xiaobei","middleName":"","lastName":"Luo","suffix":""},{"id":271045032,"identity":"5a850a2a-7dc5-4666-a344-a8f0e1114884","order_by":11,"name":"Dali Wang","email":"","orcid":"","institution":"Zhuhai People's Hospital","correspondingAuthor":false,"prefix":"","firstName":"Dali","middleName":"","lastName":"Wang","suffix":""},{"id":271045033,"identity":"03b19da0-209a-418e-88fd-35501a02aeb6","order_by":12,"name":"Xiaohong Xu","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA/UlEQVRIiWNgGAWjYJCCA2DEwNhg/MPAhoGNBC3MB4oZCtKI0wLRxcCW8Jnhw2HCag1u5Bge+FFxR86cf43h5gKD8/Z80s0PGH5UbMOpRXJGWsLBnjPPjC1nvDE2nmFwO7FN5pgBY8+Z2zi18EskHzjA23Y4ccONM2YGPAa3E9gkEgyYGdtwa2GTSGw4+BeixfwHj8E5ezaJ9A94tYBsOQy25XxbgjGPwQHGNokc/LZI9jxLOCxz5rCxwQ3mA4YzDJITgVoKDuLzi8HxHOOPbyoOyxmcP9hg8OGPnb38jPSND35U4NaCABIJCPYBItSDfEWkulEwCkbBKBh5AAA6/GDQwi6OvwAAAABJRU5ErkJggg==","orcid":"","institution":"Zhuhai People's Hospital","correspondingAuthor":true,"prefix":"","firstName":"Xiaohong","middleName":"","lastName":"Xu","suffix":""}],"badges":[],"createdAt":"2024-02-02 02:29:07","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-3919359/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-3919359/v1","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1186/s13099-024-00652-6","type":"published","date":"2024-10-30T16:13:17+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":68207055,"identity":"7b40fdfc-520e-429b-8e31-606b45789bad","added_by":"auto","created_at":"2024-11-04 16:34:27","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1871290,"visible":true,"origin":"","legend":"","description":"","filename":"Basedonmultiomicsanalysisofgutmicrobiotacharacteristicsinpatientswithacutepancreatitisandexploringthepossiblemechanismofitsexacerbationtrendfromthelevelofmicrobiotaanditsmetabolites.pdf","url":"https://assets-eu.researchsquare.com/files/rs-3919359/v1_covered_2f15ed33-c021-452b-9fcd-08e297fd49b8.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Based on multi omics analysis of gut microbiota characteristics in patients with acute pancreatitis and exploring the possible mechanism of its exacerbation trend from the level of microbiota and its metabolites","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"gut-pathogens","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"gutp","sideBox":"Learn more about [Gut Pathogens](http://gutpathogens.biomedcentral.com/)","snPcode":"13099","submissionUrl":"https://submission.nature.com/new-submission/13099/3","title":"Gut Pathogens","twitterHandle":"@GutPathogens","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"BMC/SO AJ","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"Acute pancreatitis, Gastrointestinal microbiota, Metabolites/metabolic pathways, Severe trend, Multiple omics","lastPublishedDoi":"10.21203/rs.3.rs-3919359/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-3919359/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"Specific intestinal metabolites are closely associated with the classification, severity, and necrosis of acute pancreatitis (AP), providing novel insights for in-depth clinical investigation of AP. In this study, the gut microbiota and metabolites of 49 AP patients at different treatment stages and severities were analyzed using 16s RNA sequencing and untargeted metabolomics to investigate the trends observed in severe AP based on gut microbiota composition and metabolome profiles. The findings revealed an imbalance in intestinal flora homeostasis among AP patients characterized by a decrease in probiotics and an increase in opportunistic pathogens, leading to damage to the intestinal mucosal barrier through reduced short-chain fatty acid (SCFA) secretion and disruption of the intestinal epithelium. This dysbiosis further influenced energy metabolism, anti-inflammatory responses, and immune regulation while also highlighting significant differences in energy metabolic pathways. These results suggest that differential composition of intestinal flora along with alterations in intestinal metabolites and metabolic pathways contribute to compromised integrity of the intestinal mucosal barrier as well as disturbances in energy metabolism within severe AP patients.","manuscriptTitle":"Based on multi omics analysis of gut microbiota characteristics in patients with acute pancreatitis and exploring the possible mechanism of its exacerbation trend from the level of microbiota and its metabolites","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-02-06 15:10:14","doi":"10.21203/rs.3.rs-3919359/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2024-03-19T18:32:20+00:00","index":"","fulltext":""},{"type":"reviewerAgreed","content":"e4b1f252-b2b7-4362-98bd-3a3fd9ec1f0a","date":"2024-03-14T01:55:19+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2024-03-13T20:33:16+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"1464bbfa-8c5a-4950-b4fd-9a588dc2c284","date":"2024-02-20T18:14:59+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2024-02-14T15:40:39+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2024-02-05T04:52:47+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2024-02-05T04:52:47+00:00","index":"","fulltext":""},{"type":"submitted","content":"Gut Pathogens","date":"2024-02-02T02:18:17+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"gut-pathogens","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"gutp","sideBox":"Learn more about [Gut Pathogens](http://gutpathogens.biomedcentral.com/)","snPcode":"13099","submissionUrl":"https://submission.nature.com/new-submission/13099/3","title":"Gut Pathogens","twitterHandle":"@GutPathogens","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"BMC/SO AJ","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"95e89d7a-55e7-4f4f-8742-c4949a42076f","owner":[],"postedDate":"February 6th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[],"tags":[],"updatedAt":"2024-11-04T16:24:34+00:00","versionOfRecord":{"articleIdentity":"rs-3919359","link":"https://doi.org/10.1186/s13099-024-00652-6","journal":{"identity":"gut-pathogens","isVorOnly":false,"title":"Gut Pathogens"},"publishedOn":"2024-10-30 16:13:17","publishedOnDateReadable":"October 30th, 2024"},"versionCreatedAt":"2024-02-06 15:10:14","video":"","vorDoi":"10.1186/s13099-024-00652-6","vorDoiUrl":"https://doi.org/10.1186/s13099-024-00652-6","workflowStages":[]},"version":"v1","identity":"rs-3919359","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-3919359","identity":"rs-3919359","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}