Separation of Microalgae from Bacterial Contaminants using Spiral Microchannel in the Presence of a Chemoattractant

Separation of Microalgae from Bacterial Contaminants using Spiral Microchannel in the Presence of a Chemoattractant | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Separation of Microalgae from Bacterial Contaminants using Spiral Microchannel in the Presence of a Chemoattractant Ahmed L. Abdel-Mawgood, Leticia F. Ngum, Y. Matsushita, Samir F. El-Mashtoly, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3819436/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 13 Apr, 2024 Read the published version in Bioresources and Bioprocessing → Version 1 posted 4 You are reading this latest preprint version Abstract Cell separation using microfluidics has become an effective method to isolate biological contaminants from bodily fluids and cell cultures, such as isolating bacteria contaminants from microalgae cultures and isolating bacteria contaminants from white blood cells. In this study, bacteria cell was used as a model contaminant in microalgae culture in a passive microfluidics device, which relies on hydrodynamic forces to demonstrate the separation of microalgae from bacteria contaminants in U and W-shaped cross-section spiral microchannel fabricated by defocusing CO 2 laser ablation. At a flow rate of 0.7 ml/min in the presence of glycine as bacteria chemoattractant, the spiral microfluidics devices with U and W-shaped cross -sections were able to isolate microalgae (Desmodesmus sp) from bacteria ( E. coli ) with a high separation efficiency of 92% and 96% respectively. At the same flow rate in the absence of glycine, the separation efficiency of microalgae for U- and W-shaped cross sections were 91% and 96% respectively. It was found that the spiral microchannel device with a W-shaped cross-section with a barrier in the center of the channel showed significantly higher separation efficiency. Spiral microchannel chips with U- or W-shaped cross sections were easy to fabricate and exhibited high throughput. With these advantages, these devices could be widely applicable to other cell separation applications, such as separating circulating tumor cells from blood. W-shaped cross-section CO2 laser ablation Glycine Separation efficiency Removal ratio Full Text Cite Share Download PDF Status: Published Journal Publication published 13 Apr, 2024 Read the published version in Bioresources and Bioprocessing → Version 1 posted Reviewers agreed at journal 08 Jan, 2024 Reviewers invited by journal 08 Jan, 2024 Editor assigned by journal 03 Jan, 2024 First submitted to journal 28 Dec, 2023 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3819436","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":265931863,"identity":"1693045f-f07e-4ff7-801f-32a6710e3cc5","order_by":0,"name":"Ahmed L. 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In this study, bacteria cell was used as a model contaminant in microalgae culture in a passive microfluidics device, which relies on hydrodynamic forces to demonstrate the separation of microalgae from bacteria contaminants in U and W-shaped cross-section spiral microchannel fabricated by defocusing CO\u003csub\u003e2\u003c/sub\u003e laser ablation. At a flow rate of 0.7 ml/min in the presence of glycine as bacteria chemoattractant, the spiral microfluidics devices with U and W-shaped cross -sections were able to isolate microalgae \u003cem\u003e(Desmodesmus sp)\u003c/em\u003e from bacteria (\u003cem\u003eE. coli\u003c/em\u003e) with a high separation efficiency of 92% and 96% respectively. At the same flow rate in the absence of glycine, the separation efficiency of microalgae for U- and W-shaped cross sections were 91% and 96% respectively. 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