The Study of XiaoChaiHu Decoction on Ectopic Endometrium Cell Apoptosis

Objective To explore the effect of XiaoChaiHu Decoction on ectopic endometrium cell apoptosis in rats.Methods Using the rat model,ectopic endometrium was evaluated by electron microscope.Fas and Caspase-3 protein expression in the endometrium and endometriotic tissue were observed by immunohistochemical method.Results The glandular cell in the endometrial implant after therapy with middle or high-dose of(10,15 g·kg-1·d-1) XiaoChaiHu Decoction showed characteristic features of apoptosis which displayed by the cell decreased in size,karyopyknosis,cytoplasmic and nuclear chromatin condensation,density increased and a lot of apoptotic bodies among cell.Whereas some stromal cell displayed degeneration and necrosis.The protein expression of Fas and Caspase-3 in endometriotic tissue of XiaoChaiHu Decoction group was higher than that in the endometrium.Conclusion The therapeutic mechanism of XiaoChaiHu Decoction for endometriosis may be related to promoting the apoptosis in endometriotic tissue by increasing Fas protein expression.