Generalized cell phenotyping for spatial proteomics with language-informed vision models

Abstract We present DeepCell Types, a novel approach to cell phenotyping for spatial proteomics that addresses the challenge of generalization across diverse datasets with varying marker panels collected across different platforms. Our approach utilizes a transformer with channel-wise attention to create a language-informed vision model; this model’s semantic understanding of the underlying marker panel enables it to learn from and adapt to heterogeneous datasets. Leveraging a curated, diverse dataset named Expanded TissueNet with cell type labels spanning the literature and the NIH Human BioMolecular Atlas Program (HuBMAP) consortium, our model demonstrates robust performance across various cell types, tissues, and imaging modalities. Comprehensive benchmarking shows superior accuracy and generalizability of our method compared to existing methods. This work significantly advances automated spatial proteomics analysis, offering a generalizable and scalable solution for cell phenotyping that meets the demands of multiplexed imaging data. Competing Interest Statement DVV is a co-founder of Aizen Therapeutics and holds equity in the company. Footnotes Added Ahamed Raffey Iqbal to the author list; Included more datasets; Clarification and additional benchmark analysis.