The Study of the Interaction of Chitosan NPs with Epithelial Cultures: A Simplified Scheme of Wound Healing at a Cellular Level

Chitosan is naturally considered an excellent biomaterial for wound healing treatment. In the present work, the synthesis and physicochemical characterization of three different average sizes of CsNPs were achieved (15, 50 and 125 nanometers) with the purpose of studying the in vitro interaction with Madin-Darby canine kidney (MDCK) cells. From our results we found that CsNPs have a crystalline structure with a diameter size less than 15 nm. In cell viability we observed that the three average sizes of CsNPs were not toxic at a concentration of 400 µg/ml. Moreover, it was found on wound healing assays that 125 nm CsNPs induced the best results for cell migration. Besides, proteomic analysis showed the expression and overexpression of proteins induced by the presence of CsNPs in MDCK cells.