Perturbation of periodic spot-generation balance leads to diversified pigmentation patterning of harlequin Phalaenopsis orchids: in silico prediction

Perturbation of periodic spot-generation balance leads to diversified pigmentation patterning of harlequin Phalaenopsis orchids: in silico prediction | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Perturbation of periodic spot-generation balance leads to diversified pigmentation patterning of harlequin Phalaenopsis orchids: in silico prediction Ti-Wen Lu, Wen-Huei Chen, Pao-Yang Chen, Yu-Chen Shu, Hong-Hwa Chen This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4274590/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 18 Jul, 2024 Read the published version in BMC Plant Biology → Version 1 posted 10 You are reading this latest preprint version Abstract Harlequin Phalaenopsis orchids are known for unprecedented clownish dark reddish-purple patches. The unreproducible diversified pigmentation patterning of harlequin flowers is an obstacle to commercialization. Insertion of a retrotransposon HORT1 in the promoter of a transcription activator for spotted pigmentation, PeMYB11, explains the patterning diversity. Nonetheless, in the absence of solo-LTR of HORT1, a transcriptional enhancer of PeMYB11, purple patches can still be generated in the presence of full-length HORT1, a transcriptional silencer of PeMYB11. Intriguingly, PeMYBx, a repressor for anthocyanin accumulation, is highly enriched in the purple region. Meanwhile, miR858 significantly expresses in the white region of harlequin flowers. To fill the knowledge gap, we refined the biological model for generating anthocyanin spots in Phalaenopsis, and then mathematically modeled the interactions with additional considerations of full-length HORT1 and the solo-LTR of HORT1, and altered amount of miR858. Our simulation suggests that not only the solo-LTR of HORT1 but also the reduced amount of miR858 take charge of the generation of purple patches but in an antagonistic manner. Our work extends the current understanding of how HORT1 and miR858 are involved in regulating the pigmentation patterning of harlequin Phalaenopsis and provides an experimental framework for future research. harlequin Phalaenopsis retrotransposon HORT1 microRNA (miRNA) pigmentation patterning Turing patterns Full Text Additional Declarations No competing interests reported. Supplementary Files SupplementaryFigure030724N.pdf Cite Share Download PDF Status: Published Journal Publication published 18 Jul, 2024 Read the published version in BMC Plant Biology → Version 1 posted Editorial decision: Revision requested 20 May, 2024 Reviews received at journal 15 May, 2024 Reviewers agreed at journal 13 May, 2024 Reviews received at journal 09 May, 2024 Reviewers agreed at journal 29 Apr, 2024 Reviewers invited by journal 29 Apr, 2024 Editor invited by journal 22 Apr, 2024 Submission checks completed at journal 22 Apr, 2024 Editor assigned by journal 22 Apr, 2024 First submitted to journal 16 Apr, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4274590","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":294173375,"identity":"e8622f82-f8b7-48fa-9971-8dd15c8b7f6a","order_by":0,"name":"Ti-Wen Lu","email":"","orcid":"","institution":"National Cheng Kung University","correspondingAuthor":false,"prefix":"","firstName":"Ti-Wen","middleName":"","lastName":"Lu","suffix":""},{"id":294173377,"identity":"7ad3870a-714b-4241-931a-3d7d95f91009","order_by":1,"name":"Wen-Huei Chen","email":"","orcid":"","institution":"National Cheng Kung 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Our simulation suggests that not only the solo-LTR of HORT1 but also the reduced amount of miR858 take charge of the generation of purple patches but in an antagonistic manner. 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