Simultaneous determination of moisture, 1,2-propanediol, glycerol and menthol in tobacco products using GC‒TCD&FID in parallel method

Simultaneous determination of moisture, 1,2-propanediol, glycerol and menthol in tobacco products using GC‒TCD&FID in parallel method | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Simultaneous determination of moisture, 1,2-propanediol, glycerol and menthol in tobacco products using GC‒TCD&FID in parallel method Yaping Ma, Qinlin Xiao, Li Li, Juan Yang, Jing Wen, Yuyang Deng, and 4 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6111429/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 09 Jul, 2025 Read the published version in Scientific Reports → Version 1 posted 8 You are reading this latest preprint version Abstract Moisture, 1,2-propanediol, and glycerol are crucial quality indicators that often require simultaneous determination during the production and application of novel tobacco products and reconstituted tobacco leaves. However, the inability to accurately measure these components simultaneously poses a significant challenge. This study innovatively proposes the use of GC-TCD&FID for the simultaneous determination of moisture, 1,2-propanediol, glycerol and menthol. The results showed that: (1) The standard curves of the four components had good linearity ( the correlation coefficient R 2 for water > 0.995, and R 2 for the three alcohols > 0.999), with recoveries ranging from 94.49–102.83%, repeatability RSD ranging from 1.25–2.33%, limits of detection for water was 0.32mg/mL and limits of detection for the three alcohols ranging from 0.04mg/mL to 0.21mg/mL. (2) The process of sample pretreatment and GC analysis has been consolidated from two separate steps into one, thereby reducing the workload, the consumption of reagents and time required for both sample pretreatment and GC analysis by about 50%, and anhydrous ethanol instead of methanol was used as the solvent, meeting the requirements for green, low‒carbon, and sustainable development. This method has been validated and applied for one year, meeting the production requirements. Physical sciences/Chemistry Physical sciences/Engineering Biological sciences/Chemical biology Reconstituted tobacco leaves GC‒TCD&FID method Glycerol 1 2‒Propanediol Menthol Water Figures Figure 1 Figure 2 Figure 3 Figure 4 1. Introduction Maintaining moisture content at a certain level has a significant impact on the storage, processing durability, and sensory comfort during smoking of tobacco and tobacco products. 1,2-Propanediol and glycerol are commonly used humectants in tobacco and tobacco products, playing a crucial role in retaining moisture content 1 , extending the shelf life of tobacco products 2 and also influencing other properties of these products. For instance, the application of glycerol has a notable effect on the thermogravimetric and heat release characteristics of cigar tobacco leaves, and it can promote the release of alkaline aroma components in cigar tobacco leaves under heating conditions 3 . For reconstructed tobacco sheets used in heated tobacco products, glycerol also serves as an aerosol former, and its addition correlates with the release of volatile and semi-volatile aroma components 4 – 5 . Meanwhile, there is an interactive relationship between the adsorption of glycerol in reconstructed tobacco sheets and their moisture content 6 . The moisture content of the tobacco core material affects the release of total particulate matter, aerosol former, and nicotine, which are the main components of heated tobacco aerosols 7 . Therefore, moisture, 1,2-propanediol, and glycerol are important quality indicators that often need to be simultaneously assessed in tobacco and tobacco products. Due to the extensive application of polyhydroxy alcohols, methods for qualitative and quantitative determination of polyhydroxy alcohols via gas chromatography (GC) and its coupled techniques have been established, which are applicable to various matrices such as inks 8 , hand sanitizers 9 , water-based coatings 10 , and Chinese liquor 11 , among others. In the tobacco industry, the oven-drying method 12 is commonly employed for moisture determination; however, in the presence of both moisture and alcohols, the interference caused by alcohols may adversely affect the accuracy of moisture measurement. Methods recommended by the Centre of Research Excellence on Tobacco Science (CORESTA), namely CRM57 13 and YC/T 345-2010 14 , utilize gas chromatography with thermal conductivity detection (GC-TCD) to determine moisture content in tobacco and tobacco products, while CRM60 15 and YC/T 243-2008 16 employ gas chromatography with flame ionization detection (GC-FID) to measure 1,2-propanediol and glycerol. Schematic diagrams of CRM57, YC/T 345—2010 and CRM60, YC/T 243—2008 are shown in Fig. 1 . Currently, neither CORESTA nor the industry possesses a standardized method for the simultaneous determination of moisture, 1,2-propanediol, and glycerol in a single test solution for routine testing and research. To ascertain the contents of these compounds in tobacco and tobacco products, two separate sample pretreatments and two gas chromatography (GC) analyses are typically required. For instance, Zhao Meili et al. 17 utilized YC/T 345—2010 14 and YC/T 243—2008 16 to measure moisture and glycerol content, respectively, during their study on the migration characteristics of moisture and glycerol in tobacco cut filler during airflow drying. Similarly, Lu Lehua et al. 18 employed CRM57 13 and CRM60 15 to determine moisture content and glycerol mass fraction, respectively, when investigating the release performance of major components in reconstituted tobacco leaf for electrically heated cigarettes processed through different techniques. This study established a method applicable to different types of tobacco and tobacco materials, which can simultaneously determine moisture, 1,2-propanediol, and glycerol, as well as menthol, an important quality indicator of mentholated tobacco materials. A schematic diagram of this method is shown in Fig. 2 . This method achieves simultaneous injection and determination of moisture and alcohol substances by constructing a gas chromatography instrument equipped with two injection towers, two chromatographic columns, and two detectors. Compared to the separate determination of moisture, 1,2-propanediol, and glycerol using CRM57, YC/T 345—2010 and CRM60, YC/T 243—2008, the established method requires only once sample pre-treatment and once GC analysis. Using absolute ethanol instead of methanol as the solvent reduces the consumption of chemical reagents and experimental consumables by approximately 50%, and reduces the sample pre-treatment time and GC analysis time by approximately 50%, meeting the requirements of green, low-carbon, and sustainable development. This method will provide a suitable approach for routine quality control testing and related research work on moisture, 1,2-propanediol, glycerol, and menthol in tobacco and tobacco products. 2. Materials and methods 2.1 Materials, reagents, and instruments Novel reconstituted tobacco leaf for heated cigarettes and conventional reconstituted tobacco leaf for cigarettes were provided by Sichuan China Tobacco Industry Co., Ltd. Anhydrous ethanol (99.9%, ACS/HPLC grade), glycerol (99%), 1,2-propanediol (99.5%, ACS grade), isopropanol (99.5%, super dry solvent) (Beijing Hwaki Chemical Co., Ltd.); menthol (99%), 1,3-butanediol (99.5%) (Sigma–Aldrich, USA); ultrapure water (self-made, resistivity ≥ 18.2 MΩ·cm); 0.45 μm organic phase filter membranes (Tianjin Jinteng Experimental Equipment Co., Ltd.); helium (purity ≥ 99.999%, V/V) (Sichuan Messer Gas Products Co., Ltd.). An 8890GC gas chromatograph equipped with TCD and FID detectors, HP-PLOT Q gas chromatography column (30 m × 0.53 mm × 40 µm), HP-INNOWax gas chromatography column (30 m × 0.25 mm × 0.25µm) (Agilent, USA); AX504 electronic balance (sensitivity 0.0001 g) (Mettler Toledo, Switzerland); ZD-88 full-temperature air bath oscillator (Shanghai Precision Instrumentation Co., Ltd.); Milli-Q ultrapure water system (Millipore, USA); 8510E-DTH ultrasonic cleaner (Branson Ultrasonics, USA). 2.2 Methods 2.2.1 Preparation of internal standard stock solution Accurately weigh 5 g of each isopropanol and 1,3-butanediol, and dilute to 50 mL with anhydrous ethanol to prepare a 100 mg/mL internal standard stock solution. 2.2.2 Preparation of extraction solvent Precisely measure 30 mL of the internal standard stock solution and dilute to 1000 mL with anhydrous ethanol to prepare a 3 mg/mL extraction solvent. 2.2.3 Preparation of moisture standard working solutions Accurately weigh 0.5 g of water and dilute to 50 mL with anhydrous ethanol to prepare a 10 mg/mL moisture standard stock solution. Precisely measure 0, 0.2, 0.5, 1.0, 2.0, 4.0, 8.0 mL of the moisture standard stock solution into different 10 mL volumetric flasks, then accurately add 0.3 mL of the internal standard stock solution to each, and dilute to volume with anhydrous ethanol. The resulting solutions have moisture concentrations of 0, 0.2, 0.5, 1.0, 2.0, 4.0, 8.0 mg/mL, respectively, with an internal standard concentration of 3 mg/mL in each. 2.2.4 Preparation of 1,2-propanediol, glycerol, and menthol standard working solutions Accurately weigh 0.2 g of 1,2-propanediol, 1.0 g of glycerol, and 0.2 g of menthol, and dilute to 50 mL with anhydrous ethanol to prepare a mixed standard stock solution with concentrations of 4, 20, and 4 mg/mL, respectively. Precisely measure 0.05, 0.2, 0.5, 1.0, 4.0, 8.0 mL of the standard stock solution into different 10 mL volumetric flasks, then accurately add 0.3 mL of the internal standard stock solution to each, and dilute to volume with anhydrous ethanol. The resulting solutions have 1,2-propanediol and menthol concentrations of 0.02, 0.08, 0.2, 0.4, 1.6, 3.2 mg/mL, respectively, glycerol concentrations of 0.1, 0.4, 1.0, 2.0, 8.0, 16.0 mg/mL, respectively, with an internal standard concentration of 3 mg/mL in each. 2.2.5 Tobacco sample preparation Sheet sample pretreatment : Randomly select 100 g of the sample to be tested, cut it into fragments of approximately 0.4 cm × 0.4 cm, thoroughly mix, and store in a sealed, light-protected container for later use. Filament sample pretreatment : Randomly select 100 g of the sample to be tested, thoroughly mix, and store in a sealed, light-protected container for later use. Preparation of test solution : Weigh 1.5 g of the sample (to 0.001 g precision) into a 50 mL stoppered conical flask, accurately add 25 mL of the extraction solvent, and oscillate and extract at 30°C and 160 r/min for 3 hours 13 . Filter the supernatant, and the filtrate is the test solution. Preparation of blank solution : Prepare a blank solution without adding the sample, following the same procedure as for the test solution, for the determination of blank moisture value. Due to the solvent's absorption of water, the blank moisture value should be subtracted when calculating the moisture content of the test solution. Unless otherwise specified in the following experiments, each sample was determined in triplicate, and the average value was taken. 2.3 GC-TCD&FID analysis Moisture determination conditions : HP-PLOT Q gas chromatography column (30 m × 0.53 mm × 40µm); TCD detector temperature: 270°C; injection port temperature: 260°C; split injection mode with a split ratio of 10:1; injection volume: 1.0 μL; carrier gas: helium (≥ 99.999%), constant flow mode, flow rate: 6.0 mL/min; make-up gas: helium, flow rate: 10 mL/min; reference flow rate: 25 mL/min. 1,2-propanediol, glycerol, and menthol determination conditions : HP-INNOWax gas chromatography column (30 m × 0.25 mm × 0.25µm); FID detector temperature: 270°C; injection port temperature: 260°C; split injection mode with a split ratio of 25:1; injection volume: 1.0 µL; carrier gas: helium (≥ 99.999%), constant flow mode: flow rate set at 1.2 mL/min; air flow rate at 400 mL/min; hydrogen flow rate at 40 mL/min; make-up gas is helium with a flow rate of 25 mL/min. Heating Procedure: initiate at a temperature of 110 ℃ and maintain for 1 minute. Subsequently, increase the temperature at a rate of 10 ℃/min to reach 150 ℃, where it is held for 2 minutes. Further elevate the temperature at a rate of 20 ℃/min to 240 ℃ and maintain for 5 minutes. 3. Results and Discussion 3.1 Selection of Sample Morphology For flake samples, the influence of two sample morphologies (4mm×4mm cuboid and powdered) on the extraction efficiency of target compounds using methanol as the extraction solvent under oscillatory extraction conditions was investigated. The results (Table 1 ) indicate that the extraction efficiency for 1,2-propanediol is approximately 6% higher in the cuboid form compared to the powdered form; for menthol, the extraction efficiency is approximately 9% higher in the cuboid form; however, for glycerol, the extraction efficiency is approximately 2% lower in the cuboid form. This may be because 1,2-propanediol has a boiling point of 187°C, and menthol has a boiling point of 216°C. During the pulverization process of tobacco samples, friction generates heat, causing more volatilization of 1,2-propanediol and menthol, which have lower boiling points, resulting in higher extraction efficiency of 1,2-propanediol and menthol from cuboid tobacco samples compared to powdered samples. In contrast, glycerol has a boiling point of 290°C and is difficult to volatilize, making powdered tobacco samples more favorable for its extraction.The extraction efficiency for moisture is comparable between the cuboid and powdered forms. Taking these considerations into account, the 4mm×4mm cuboid morphology was selected as the sample morphology for flake samples. Table 1 Effects of sample morphology on extraction efficiency of target compounds(n = 3) Sample No. Sample morphology Water content (%) 1,2-propanediol content (%) Glycerol content (%) Menthol content (%) 1 # 4 mm×4 mm cuboid 6.26 2.75 17.82 1.30 Powdered 6.29 2.58 18.16 1.18 2 # 4 mm×4 mm cuboid 6.43 3.59 17.21 2.11 Powdered 6.47 3.37 17.48 1.92 3.2 Selection of extraction solvent The extraction efficiencies of target compounds using isopropanol, anhydrous ethanol, and methanol were investigated. The results (Table 2 ) indicate that anhydrous ethanol exhibits significantly higher extraction efficiency for glycerol compared to methanol and isopropanol. Furthermore, anhydrous ethanol demonstrates slightly higher extraction efficiencies for water, 1,2-propanediol, and menthol than methanol and isopropanol. Therefore, anhydrous ethanol was selected as the extraction solvent. Table 2 Effects of extraction solvent on extraction efficiency of target compounds(n = 3) Sample No. Extraction solvent Water content (%) 1,2-propanediol content (%) Glycerol content (%) Menthol content (%) 1# Isopropanol 6.21 2.69 15.16 1.24 Anhydrous ethanol 6.26 2.75 17.82 1.30 Methanol 6.23 2.71 17.11 1.27 2# Isopropanol 6.38 3.54 14.82 2.05 Anhydrous ethanol 6.43 3.59 17.21 2.11 Methanol 6.36 3.55 16.62 2.07 3.3 Comparative determination of alcoholic compounds in the same standard solution using FID and TCD detectors Wang Kang 19 et al. established a GC-TCD method for the simultaneous detection of water, nicotine, 1,2-propanediol, glycerol, triacetin, and menthol in aerosol from heat-not-burn cigarettes. Kensheng Ye 20 et al. also developed a GC-TCD method for the simultaneous determination of water, 1,2-propanediol, nicotine, and glycerol in tobacco materials for heat-not-burn cigarettes. To assess the feasibility of using the TCD detector for routine quality control measurements of 1,2-propanediol and glycerol in tobacco and tobacco products, standard working solutions of 1,2-propanediol, glycerol, and menthol were determined using both the established method and the literature method 19 . The results (Table 3 ) show that the detection values (peak areas) of the TCD detector for 1,2-propanediol, glycerol, menthol, and 1,3-butanediol are significantly lower than those of the FID detector. The results indicate that the FID exhibits higher detection sensitivity towards these four alcoholic compounds, rendering it more suitable for routine quality control testing. Table 3 Determination of alcohols in the same standard solution by FID detector and TCD detector Target compound Concentration (mg/mL) FID detection value (Peak area) TCD detection value (Peak area) Peak area ratio (FID/TCD) 1,2-Propanediol 3.432 764.2 54.4 14.1 Glycerol 16.354 2785.3 211.6 13.2 Menthol 3.291 1621.6 35.6 45.7 1,3-Butanediol 3.013 794.9 46.2 17.2 3.4 Methodological validation Reference was made to the "Guiding Principles for Analytical Method Validation" numbered 9101 in the "General Technical Requirements" section of the 2020 edition, Part IV, of the "Pharmacopoeia of the People's Republic of China" 21 to investigate the linearity, detection limit, quantitation limit, recovery, and reproducibility of the method. 3.4.1 Linearity, detection limit, and quantitation limit The concentration ranges of the compounds in the standard working solutions were set to basically cover the content ranges of the target compounds commonly found in tobacco and tobacco products. The detection limit and quantitation limit were determined using the signal-to-noise ratio method: by comparing the signals measured from known low-concentration samples with the signals measured from blank samples, the contents of the corresponding target compounds at signal-to-noise ratios of 3:1 and 10:1 were used to determine the detection limit and quantitation limit, respectively. Linearity assessment of the water determination method: Seven levels of water standard working solutions were measured according to the water determination conditions to establish a water standard curve (with the ratio of water concentration to isopropanol concentration as the abscissa and the ratio of water peak area to isopropanol peak area as the ordinate). The linearity of the standard curve was evaluated using the correlation coefficient. Linearity assessment of the determination methods for 1,2-propanediol, glycerol, and menthol: Six levels of standard working solutions were measured according to the determination conditions for 1,2-propanediol, glycerol, and menthol, and standard curves for 1,2-propanediol, glycerol, and menthol were established respectively (with the ratio of target compound concentration to internal standard concentration as the abscissa and the ratio of target compound peak area to internal standard peak area as the ordinate). The linearity of the standard curves was evaluated using the correlation coefficient. The results (Table 4 ) indicate that the standard curves for the four target compounds exhibit good linearity, with low detection limits and quantitation limits, and high method sensitivity. The recovery rates for the three alcohols range from 94.49–102.30%, with an RSD of 1.25–2.32%. Additionally, the linearity of the standard curves mentioned above surpasses the research findings of Wang, L. Q., et al., regarding the determination of alcohols. 22 Table 4 Linear parameters, detection limits, and quantitation limits of four target compounds Target compound Linear range (mg/mL) Regression equation Correlation coefficient Detection limit (mg/mL) Quantitation limit (mg/mL) Water 0.2 ~ 9.0 y = 15.671x + 5.205 0.9987 0.32 0.97 1,2-propanediol 0.02 ~ 3.2 y = 223.927x − 1.643 0.9999 0.21 0.58 Glycerol 0.1 ~ 16.0 y = 171.310x − 7.484 0.9999 0.15 0.67 Menthol 0.02 ~ 3.2 y = 495.415x − 0.612 0.9999 0.04 0.21 3.4.2 Recovery and reproducibility of the method Recovery assessment method: A known amount of a standard compound of known purity was precisely added to a sample with a known content (such that the sum of the added amount and the original content remained within the linear range of the standard curve). The sample was then measured according to the established method. The recovery was calculated by dividing the difference between the measured value and the original content in the sample by the added amount of the standard compound. Seven parallel measurements were performed. The results (Table 5 ) demonstrate that the method exhibits high accuracy and excellent reproducibility. Table 5 Recovery and precision of the method(n = 7) Target compound Original content/mg Spike amount/mg Measured range/mg Recovery range/% RSD/% Water 93.9 70.6 67.9 ~ 72.6 96.13 ~ 102.83 2.33 1,2-Propanediol 41.25 30.3 29.3 ~ 31.0 96.80 ~ 102.30 2.32 Glycerol 267.3 200.2 191.6 ~ 198.6 95.69 ~ 99.22 1.25 Menthol 19.5 14.1 13.3 ~ 13.9 94.49 ~ 98.33 1.36 3.5 Sample analysis The tobacco material samples were analyzed according to the established method. The gas chromatogram for water, 1,2-propanediol, glycerol, and menthol determination is presented in Fig. 3 . The results indicate that the peaks of each target compound and internal standard are sharp and well-separated. The measurement results for the 16 tobacco material samples are presented in Table 6 . Specifically, samples numbered 1#~3# represent, 4#~5# for sheet-type heated tobacco products (produced via dry papermaking), 6#~13# for filament-type heated tobacco products (manufactured through the thick slurry method), and 14#~16# for filament-type conventional cigarettes. This is shown in Fig. 4 , the average moisture content follows the order: reconstituted tobacco leaves for filament-type heated tobacco products (thick slurry method) > reconstituted tobacco leaves for filament-type conventional cigarettes > reconstituted tobacco leaves for sheet-type mentholated heated tobacco products > reconstituted tobacco leaves for sheet-type heated tobacco products (dry papermaking). The average 1,2-propanediol content in reconstituted tobacco leaves for sheet-type mentholated heated tobacco products is significantly higher than that in other sample types. The average glycerol content in reconstituted tobacco leaves for filament-type conventional cigarettes is considerably lower than in other types. Notably, there is considerable variation in the menthol content among different samples of reconstituted tobacco leaves for sheet-type mentholated heated tobacco products. The component content variations are relatively small for both reconstituted tobacco leaves for sheet-type heated tobacco products (dry papermaking) and filament-type conventional cigarettes, with coefficient of variation (CV) values ranging from 0.8–7.2%. The CVs for glycerol content across all four types of tobacco material samples are also low, falling within the range of 3.1–6.7%. However, the CVs for moisture and 1,2-propanediol content in reconstituted tobacco leaves for filament-type heated tobacco products (thick slurry method) are relatively high, at 23.5% and 63.9%, respectively. Table 6 Analysis results of 16 tobacco materials No. Sample state Type Water content/% 1,2-Propanediol content/% Glycerol content/% Menthol content/% 1# Sheet-like Reconstituted tobacco leaf for menthol Heated tobacco products 6.26 2.75 17.82 1.30 2# 6.43 3.59 17.20 2.10 3# 7.90 3.25 16.17 1.73 4# Reconstituted tobacco leaf for heated tobacco products (Dry papermaking) 5.19 0.82 17.77 - 5# 5.70 0.74 18.57 - 6# Filamentous Reconstituted tobacco leaf for heated tobacco products (Dense slurry method) 6.27 0.77 18.51 - 7# 7.54 0.71 18.42 - 8# 10.96 0.34 16.75 - 9# 10.37 0.23 16.47 - 10# 6.93 0.24 16.41 - 11# 6.72 0.23 16.04 - 12# 6.29 0.24 16.18 - 13# 7.28 0.95 18.70 - 14# Reconstituted tobacco leaf for traditional cigarettes 6.92 0.56 0.67 - 15# 6.96 0.53 0.71 - 16# 6.85 0.49 0.65 - 3.6 Establishment of quality inspection technical standards for Enterprises Using the established method, quality inspection technical standards were formulated by Sichuan China Tobacco Industrial Co., Ltd. for routine quality control testing of water, 1,2-propanediol, glycerol, and menthol in various tobacco and tobacco products. The standards have been in operation for one year with satisfactory results. 4. Conclusion A GC-TCD&FID method for the simultaneous determination of moisture, 1,2-propanediol, glycerol, and menthol contents in tobacco materials was established. (1) The standard curves of the four components exhibited good linearity (correlation coefficient R > 0.995 for moisture and R > 0.999 for the three alcohols), high accuracy (recovery rates of 94.49–102.83%), good reproducibility (RSD of 1.25–2.33%), and high sensitivity (limits of detection for water was 0.32mg/mL and limits of detection for the three alcohols ranging from 0.04mg/mL to 0.21mg/mL). (2) The established method reduces the original process of twice pre-treatments + twice GC analyses to only once sample pre-treatment + once GC analysis, reducing the sample pre-treatment time and GC analysis time by approximately 50%, and using absolute ethanol instead of methanol as the solvent reduces the consumption of chemical reagents and experimental consumables by approximately 50%. (3) The established method has been in operation for one year in the quality inspection technical standards of related products at China Tobacco Sichuan Industrial Co., Ltd., with good results. Declarations Competing interests: The authors declare no competing interests. Author Contribution Writing-original draft preparation, Yaping Ma; Data curation, Qinlin Xiao and Li Li; Methodology, Juan Yang, Jing Wen and Yuyang Deng; Project administration, Jia Guo Xixiang Zhang and Wu Wen ; Writing-review and editing, Yi Shen. All authors have read and agreed to the published version of the manuscript. 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Tobacco and tobacco products–Preparation of test sample and determination of water content–Over method. (1996). https://kns.cnki.net/kcms2/article/abstract?v=2Z_8GvOTliWXbU5F9JwruaWir8p7J1dAwOV7FBmpbINXHgOjwQyW7JlUr5H0caJ6qvkIhZ4I_koVv0lsPdhvR0EPGKzk DuoHBmiDnmNIUoUDblXl_udbqvGe5p6QWJS92bR1n75KJIiIA9XrXJFHgptzYx9JRPA69AjyBlCCfrEBHILJvPLJf6RlZyhLl_1t&uniplatform=NZKPT&language=CHS(1966). CORESTA Recommended Methods No. 57. Determination of water in tobacco and tobacco products by Gas Chromatographic analysis. (2018). https://www.coresta.org/determination-water-tobacco-and-tobacco-products-gas-chromatographic-analysis-29180.html YC/T 345―2010. Tobacco and tobacco products—Determination of water content—Gas Chromatographic method. (2010). https://kns.cnki.net/kcms2/article/abstract?v=2Z_8GvOTliVaOUPgCZO7Tlc5IM7xLpXHVDfN8axxZ36gzVKXUi3yqDksdd-5l-8R0_vikgvkOLq1RfDDbGBYeYnf8rIOJ8u3G3-i5gv-Qb-pb2zqhCuaoQ6yR0EAUTLC_lNbmnt5ap4DLNJGHgIORZsyPQinJpslHEfkd4EZV1DCR0M3QiRVPgaPezr6G7Jb&uniplatform=NZKPT&language=CHS CORESTA Recommended Methods No. 60. Determination of 1,2–propylene glycol and glycerol in tobacco products by Gas Chromatographic. (2019). https://www.coresta.org/determination-12-propylene-glycol-and-glycerol-tobacco-and-tobacco-products-gas-chromatography-29183 YC/T 243―2008. Tobacco and tobacco products—Determiation of 1,2–propylene glycol and glycerol—Gas chromatographic method. (2018). https://kns.cnki.net/kcms2/article/abstract?v=2Z_8GvOTliUdaz6ftQnD6qctX-pIjqPKEbY8DDtZAZT8QAZbucd5rWB3gSBYzpBlsFiCOt2rq_8YWXS0Jf-kmxZqn-pEC7hzeYunv6msIQwhHcSrwLRfVZ1yCVkRs5N7g6X194d4sdGvVgA2kVqIs8-FvBTxWwPwbTFiNVhhhSqmn9VFU9bj0iHqRvOoX8-IzdKleFjCrbpqgtIdoKhRFA==&uniplatform=NZKPT&language=CHS Zhao, M. L. et al. Migration characteristics of moisture and glycerol in cut tobacco strips during pneumatic drying. Tob. Sci. Technol. 55 (11), 89–96. 10.16135/j.issn1002-0861.2022.0211 (2022). https://link.cnki.net/doi/ Lu, L. H. et al. Release performance of main components in reconstituted tobacco sheets by different processing techniques for electrically heated tobacco products. Tob. Sci. Technol. 56 (3), 30–40. 10.16135/j.issn1002-0861.2022.0896 (2023). https://link.cnki.net/doi/ Wang, K. et al. Simultaneous determination of moisture content and deliveries of nicotine, glycerol, 1,2–propylene glycol, glycerol triacetate and menthol in aerosol from heat–not–burn tobacco products by GC–TCD method. Tob. Sci. Technol. 52 (3), 63–68. 10.16135/j.issn1002-0861.2018.0219 (2019). https://link.cnki.net/doi/ Ken, S. Y. et al. Simultaneous determination of moisture, 1,2–propylene glycol, nicotine and glycerol in tobacco material for heat–not–burn products by GC–TCD. Tob. Sci. Technol. 53 (5), 41–46. 10.16135/j.issn1002-0861.2019.0267 (2020). https://link.cnki.net/doi/ Chinese Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China, 2020 Edition Four Parts. China Pharm. Sci. Technol. Press. 480–483 (2020). Wang, L., Cardenas, R. B. & Watson, C. An isotope dilution ultra high performance liquid chromatography-tandem mass spectrometry method for the simultaneous determination of sugars and humectants in tobacco products. J. Chromatogr. A . 1514 , 95–102. https://doi.org/10.1016/j.chroma.2017.07.079 (2017). Additional Declarations No competing interests reported. Cite Share Download PDF Status: Published Journal Publication published 09 Jul, 2025 Read the published version in Scientific Reports → Version 1 posted Editorial decision: Revision requested 14 May, 2025 Reviews received at journal 01 May, 2025 Reviews received at journal 29 Apr, 2025 Reviewers agreed at journal 24 Apr, 2025 Reviewers agreed at journal 21 Apr, 2025 Reviewers invited by journal 21 Apr, 2025 Submission checks completed at journal 20 Apr, 2025 First submitted to journal 17 Apr, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6111429","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":445836088,"identity":"07504337-d4f4-4073-8355-c76b97fcfa99","order_by":0,"name":"Yaping Ma","email":"","orcid":"","institution":"Technology Center, China Tobacco Sichuan Industrial Co., Ltd.","correspondingAuthor":false,"prefix":"","firstName":"Yaping","middleName":"","lastName":"Ma","suffix":""},{"id":445836090,"identity":"e5f4ddf2-9718-4c7d-95b1-46d62f768274","order_by":1,"name":"Qinlin Xiao","email":"","orcid":"","institution":"Technology Center, China Tobacco Sichuan Industrial Co., Ltd.","correspondingAuthor":false,"prefix":"","firstName":"Qinlin","middleName":"","lastName":"Xiao","suffix":""},{"id":445836092,"identity":"588f776c-8dc1-4e1e-8b97-723cca009a83","order_by":2,"name":"Li Li","email":"","orcid":"","institution":"Technology Center, China Tobacco Sichuan Industrial Co., Ltd.","correspondingAuthor":false,"prefix":"","firstName":"Li","middleName":"","lastName":"Li","suffix":""},{"id":445836093,"identity":"6a0aea75-0cf4-4578-9f74-e26767ae3278","order_by":3,"name":"Juan Yang","email":"","orcid":"","institution":"Technology Center, China Tobacco Sichuan Industrial Co., Ltd.","correspondingAuthor":false,"prefix":"","firstName":"Juan","middleName":"","lastName":"Yang","suffix":""},{"id":445836094,"identity":"04a89461-9c82-437f-9e09-1b351b08d272","order_by":4,"name":"Jing Wen","email":"","orcid":"","institution":"Technology Center, China Tobacco Sichuan Industrial Co., Ltd.","correspondingAuthor":false,"prefix":"","firstName":"Jing","middleName":"","lastName":"Wen","suffix":""},{"id":445836095,"identity":"b2c48e0e-9259-46ea-a7e5-722b015eb250","order_by":5,"name":"Yuyang Deng","email":"","orcid":"","institution":"Technology Center, China Tobacco Sichuan Industrial Co., Ltd.","correspondingAuthor":false,"prefix":"","firstName":"Yuyang","middleName":"","lastName":"Deng","suffix":""},{"id":445836097,"identity":"8fbbada9-b333-4d9d-9ac5-a040ceed74f3","order_by":6,"name":"Jia Guo","email":"","orcid":"","institution":"Technology Center, China Tobacco Sichuan Industrial Co., Ltd.","correspondingAuthor":false,"prefix":"","firstName":"Jia","middleName":"","lastName":"Guo","suffix":""},{"id":445836098,"identity":"d534f6cf-f905-4d6e-bec3-4ee96c0c60e9","order_by":7,"name":"Xixiang Zhang","email":"","orcid":"","institution":"Technology Center, China Tobacco Sichuan Industrial Co., Ltd.","correspondingAuthor":false,"prefix":"","firstName":"Xixiang","middleName":"","lastName":"Zhang","suffix":""},{"id":445836104,"identity":"21784cbc-26ed-4127-9f4d-76a345ad5cc6","order_by":8,"name":"Wu Wen","email":"","orcid":"","institution":"Technology Center, China Tobacco Sichuan Industrial Co., Ltd.","correspondingAuthor":false,"prefix":"","firstName":"Wu","middleName":"","lastName":"Wen","suffix":""},{"id":445836111,"identity":"b61ebb07-e78f-410d-bcf4-b1f2f1a25e6d","order_by":9,"name":"Yi Shen","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAzElEQVRIiWNgGAWjYBACfvb+xw8+VNgws7E3EKlFsucMm+GMM2nsfDwHiNRiMCOHQZq37TC/nEQCsVokcg8Y8Jw5LM0m+XjjDYYam2iCWsx53iU8kKhIN2aTTiu2YDiWlttASItle4KBgcEZ62Q26RwzCcaGw4S1GBxIMJBIbGOub5M8Q6yWEzkGEgfbnJnZJHiI1CLZcyzNsOFMGjMbD9AvCcT4hZ+9+fDjP8ColG8/vPHGhxobwlpQHEl01CBpIVXHKBgFo2AUjAwAAEY/PshwrKixAAAAAElFTkSuQmCC","orcid":"","institution":"Technology Center, China Tobacco Sichuan Industrial Co., Ltd.","correspondingAuthor":true,"prefix":"","firstName":"Yi","middleName":"","lastName":"Shen","suffix":""}],"badges":[],"createdAt":"2025-02-26 08:53:26","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-6111429/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-6111429/v1","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1038/s41598-025-09092-x","type":"published","date":"2025-07-09T15:57:40+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":81092887,"identity":"0f0afddc-8ad6-4e8e-8443-ef5112d5d00e","added_by":"auto","created_at":"2025-04-22 07:26:16","extension":"png","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":350061,"visible":true,"origin":"","legend":"\u003cp\u003eStructure of CRM57, YC/T 345—2010 and CRM60, YC/T 243—2008\u003c/p\u003e","description":"","filename":"floatimage1.png","url":"https://assets-eu.researchsquare.com/files/rs-6111429/v1/b07c63435ac77d9579e1dab9.png"},{"id":81091998,"identity":"00aceab7-684f-4da3-aa33-a4cb2c15ae93","added_by":"auto","created_at":"2025-04-22 07:18:16","extension":"png","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":216851,"visible":true,"origin":"","legend":"\u003cp\u003eSchematic Diagram of the Established GC-TCD\u0026amp;FID Method\u003c/p\u003e","description":"","filename":"floatimage2.png","url":"https://assets-eu.researchsquare.com/files/rs-6111429/v1/0acdd2a42ff4cc47fdf9a86b.png"},{"id":81091999,"identity":"3f9c300a-d545-4c29-bec3-bd1fe4bc53ed","added_by":"auto","created_at":"2025-04-22 07:18:16","extension":"jpg","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":29251,"visible":true,"origin":"","legend":"\u003cp\u003eGas chromatogram for water 1,2‒propanediol, glycerol, and menthol detection in tobacco materials\u003c/p\u003e","description":"","filename":"3.jpg","url":"https://assets-eu.researchsquare.com/files/rs-6111429/v1/aa5f91ab6dc9f3c548a5e962.jpg"},{"id":81092006,"identity":"dfd6b10d-79da-44b7-8ea6-6f24e6939990","added_by":"auto","created_at":"2025-04-22 07:18:16","extension":"jpeg","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":194541,"visible":true,"origin":"","legend":"\u003cp\u003eBoxplots of water,1,2‒propanediol, glycerol for four types of tobacco products. Error bars at the top of each column indicate the standard deviation of the measurement.\u003c/p\u003e","description":"","filename":"floatimage4.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-6111429/v1/7a4b3d2807a3a7b7ff675946.jpeg"},{"id":86699398,"identity":"1aaffed1-963c-4ca4-af7b-8ec3056c2293","added_by":"auto","created_at":"2025-07-14 16:08:54","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1966323,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-6111429/v1/fa9992ef-ae73-4e75-b97b-c44fd79ddfa3.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Simultaneous determination of moisture, 1,2-propanediol, glycerol and menthol in tobacco products using GC‒TCD\u0026FID in parallel method","fulltext":[{"header":"1. Introduction","content":"\u003cp\u003eMaintaining moisture content at a certain level has a significant impact on the storage, processing durability, and sensory comfort during smoking of tobacco and tobacco products. 1,2-Propanediol and glycerol are commonly used humectants in tobacco and tobacco products, playing a crucial role in retaining moisture content\u003csup\u003e\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e\u003c/sup\u003e, extending the shelf life of tobacco products\u003csup\u003e\u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e\u003c/sup\u003e and also influencing other properties of these products. For instance, the application of glycerol has a notable effect on the thermogravimetric and heat release characteristics of cigar tobacco leaves, and it can promote the release of alkaline aroma components in cigar tobacco leaves under heating conditions\u003csup\u003e\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e\u003c/sup\u003e. For reconstructed tobacco sheets used in heated tobacco products, glycerol also serves as an aerosol former, and its addition correlates with the release of volatile and semi-volatile aroma components\u003csup\u003e\u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR5\" class=\"CitationRef\"\u003e5\u003c/span\u003e\u003c/sup\u003e. Meanwhile, there is an interactive relationship between the adsorption of glycerol in reconstructed tobacco sheets and their moisture content\u003csup\u003e\u003cspan citationid=\"CR6\" class=\"CitationRef\"\u003e6\u003c/span\u003e\u003c/sup\u003e. The moisture content of the tobacco core material affects the release of total particulate matter, aerosol former, and nicotine, which are the main components of heated tobacco aerosols\u003csup\u003e\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e\u003c/sup\u003e. Therefore, moisture, 1,2-propanediol, and glycerol are important quality indicators that often need to be simultaneously assessed in tobacco and tobacco products. Due to the extensive application of polyhydroxy alcohols, methods for qualitative and quantitative determination of polyhydroxy alcohols via gas chromatography (GC) and its coupled techniques have been established, which are applicable to various matrices such as inks\u003csup\u003e\u003cspan citationid=\"CR8\" class=\"CitationRef\"\u003e8\u003c/span\u003e\u003c/sup\u003e, hand sanitizers\u003csup\u003e\u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e9\u003c/span\u003e\u003c/sup\u003e, water-based coatings\u003csup\u003e\u003cspan citationid=\"CR10\" class=\"CitationRef\"\u003e10\u003c/span\u003e\u003c/sup\u003e, and Chinese liquor\u003csup\u003e\u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e\u003c/sup\u003e, among others. In the tobacco industry, the oven-drying method\u003csup\u003e\u003cspan citationid=\"CR12\" class=\"CitationRef\"\u003e12\u003c/span\u003e\u003c/sup\u003e is commonly employed for moisture determination; however, in the presence of both moisture and alcohols, the interference caused by alcohols may adversely affect the accuracy of moisture measurement. Methods recommended by the Centre of Research Excellence on Tobacco Science (CORESTA), namely CRM57\u003csup\u003e13\u003c/sup\u003e and YC/T 345-2010\u003csup\u003e14\u003c/sup\u003e, utilize gas chromatography with thermal conductivity detection (GC-TCD) to determine moisture content in tobacco and tobacco products, while CRM60\u003csup\u003e15\u003c/sup\u003e and YC/T 243-2008\u003csup\u003e16\u003c/sup\u003e employ gas chromatography with flame ionization detection (GC-FID) to measure 1,2-propanediol and glycerol. Schematic diagrams of CRM57, YC/T 345\u0026mdash;2010 and CRM60, YC/T 243\u0026mdash;2008 are shown in Fig.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003e.\u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003cp\u003eCurrently, neither CORESTA nor the industry possesses a standardized method for the simultaneous determination of moisture, 1,2-propanediol, and glycerol in a single test solution for routine testing and research. To ascertain the contents of these compounds in tobacco and tobacco products, two separate sample pretreatments and two gas chromatography (GC) analyses are typically required. For instance, Zhao Meili et al.\u003csup\u003e\u003cspan citationid=\"CR17\" class=\"CitationRef\"\u003e17\u003c/span\u003e\u003c/sup\u003e utilized YC/T 345\u0026mdash;2010\u003csup\u003e14\u003c/sup\u003e and YC/T 243\u0026mdash;2008\u003csup\u003e16\u003c/sup\u003e to measure moisture and glycerol content, respectively, during their study on the migration characteristics of moisture and glycerol in tobacco cut filler during airflow drying. Similarly, Lu Lehua et al.\u003csup\u003e\u003cspan citationid=\"CR18\" class=\"CitationRef\"\u003e18\u003c/span\u003e\u003c/sup\u003e employed CRM57\u003csup\u003e13\u003c/sup\u003e and CRM60\u003csup\u003e15\u003c/sup\u003e to determine moisture content and glycerol mass fraction, respectively, when investigating the release performance of major components in reconstituted tobacco leaf for electrically heated cigarettes processed through different techniques.\u003c/p\u003e \u003cp\u003eThis study established a method applicable to different types of tobacco and tobacco materials, which can simultaneously determine moisture, 1,2-propanediol, and glycerol, as well as menthol, an important quality indicator of mentholated tobacco materials. A schematic diagram of this method is shown in Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003e. This method achieves simultaneous injection and determination of moisture and alcohol substances by constructing a gas chromatography instrument equipped with two injection towers, two chromatographic columns, and two detectors. Compared to the separate determination of moisture, 1,2-propanediol, and glycerol using CRM57, YC/T 345\u0026mdash;2010 and CRM60, YC/T 243\u0026mdash;2008, the established method requires only once sample pre-treatment and once GC analysis. Using absolute ethanol instead of methanol as the solvent reduces the consumption of chemical reagents and experimental consumables by approximately 50%, and reduces the sample pre-treatment time and GC analysis time by approximately 50%, meeting the requirements of green, low-carbon, and sustainable development. This method will provide a suitable approach for routine quality control testing and related research work on moisture, 1,2-propanediol, glycerol, and menthol in tobacco and tobacco products.\u003c/p\u003e \u003cp\u003e \u003c/p\u003e"},{"header":"2. Materials and methods","content":"\u003cp\u003e\u003cstrong\u003e2.1 Materials, reagents, and instruments\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eNovel reconstituted tobacco leaf for heated cigarettes and conventional reconstituted tobacco leaf for cigarettes were provided by Sichuan China Tobacco Industry Co., Ltd.\u003c/p\u003e\n\u003cp\u003eAnhydrous ethanol (99.9%, ACS/HPLC grade), glycerol (99%), 1,2-propanediol (99.5%, ACS grade), isopropanol (99.5%, super dry solvent) (Beijing Hwaki Chemical Co., Ltd.); menthol (99%), 1,3-butanediol (99.5%) (Sigma\u0026ndash;Aldrich, USA); ultrapure water (self-made, resistivity \u0026ge; 18.2 M\u0026Omega;\u0026middot;cm); 0.45 \u0026mu;m organic phase filter membranes (Tianjin Jinteng Experimental Equipment Co., Ltd.); helium (purity \u0026ge; 99.999%, V/V) (Sichuan Messer Gas Products Co., Ltd.).\u003c/p\u003e\n\u003cp\u003eAn 8890GC gas chromatograph equipped with TCD and FID detectors, HP-PLOT Q gas chromatography column (30 m \u0026times; 0.53 mm \u0026times; 40 \u0026micro;m), HP-INNOWax gas chromatography column (30 m \u0026times; 0.25 mm \u0026times; 0.25\u0026micro;m) (Agilent, USA); AX504 electronic balance (sensitivity 0.0001 g) (Mettler Toledo, Switzerland); ZD-88 full-temperature air bath oscillator (Shanghai Precision Instrumentation Co., Ltd.); Milli-Q ultrapure water system (Millipore, USA); 8510E-DTH ultrasonic cleaner (Branson Ultrasonics, USA).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.2 Methods\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.2.1 Preparation of internal standard stock solution\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAccurately weigh 5 g of each isopropanol and 1,3-butanediol, and dilute to 50 mL with anhydrous ethanol to prepare a 100 mg/mL internal standard stock solution.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.2.2 Preparation of extraction solvent\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003ePrecisely measure 30 mL of the internal standard stock solution and dilute to 1000 mL with anhydrous ethanol to prepare a 3 mg/mL extraction solvent.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.2.3 Preparation of moisture standard working solutions\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAccurately weigh 0.5 g of water and dilute to 50 mL with anhydrous ethanol to prepare a 10 mg/mL moisture standard stock solution. Precisely measure 0, 0.2, 0.5, 1.0, 2.0, 4.0, 8.0 mL of the moisture standard stock solution into different 10 mL volumetric flasks, then accurately add 0.3 mL of the internal standard stock solution to each, and dilute to volume with anhydrous ethanol. The resulting solutions have moisture concentrations of 0, 0.2, 0.5, 1.0, 2.0, 4.0, 8.0 mg/mL, respectively, with an internal standard concentration of 3 mg/mL in each.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.2.4 Preparation of 1,2-propanediol, glycerol, and menthol standard working solutions\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAccurately weigh 0.2 g of 1,2-propanediol, 1.0 g of glycerol, and 0.2 g of menthol, and dilute to 50 mL with anhydrous ethanol to prepare a mixed standard stock solution with concentrations of 4, 20, and 4 mg/mL, respectively. Precisely measure 0.05, 0.2, 0.5, 1.0, 4.0, 8.0 mL of the standard stock solution into different 10 mL volumetric flasks, then accurately add 0.3 mL of the internal standard stock solution to each, and dilute to volume with anhydrous ethanol. The resulting solutions have 1,2-propanediol and menthol concentrations of 0.02, 0.08, 0.2, 0.4, 1.6, 3.2 mg/mL, respectively, glycerol concentrations of 0.1, 0.4, 1.0, 2.0, 8.0, 16.0 mg/mL, respectively, with an internal standard concentration of 3 mg/mL in each.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.2.5 Tobacco sample preparation\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eSheet sample pretreatment\u003c/strong\u003e: Randomly select 100 g of the sample to be tested, cut it into fragments of approximately 0.4 cm \u0026times; 0.4 cm, thoroughly mix, and store in a sealed, light-protected container for later use.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFilament sample pretreatment\u003c/strong\u003e: Randomly select 100 g of the sample to be tested, thoroughly mix, and store in a sealed, light-protected container for later use.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003ePreparation of test solution\u003c/strong\u003e: Weigh 1.5 g of the sample (to 0.001 g precision) into a 50 mL stoppered conical flask, accurately add 25 mL of the extraction solvent, and oscillate and extract at 30\u0026deg;C and 160 r/min for 3 hours\u003csup\u003e13\u003c/sup\u003e. Filter the supernatant, and the filtrate is the test solution.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003ePreparation of blank solution\u003c/strong\u003e: Prepare a blank solution without adding the sample, following the same procedure as for the test solution, for the determination of blank moisture value. Due to the solvent\u0026apos;s absorption of water, the blank moisture value should be subtracted when calculating the moisture content of the test solution.\u003c/p\u003e\n\u003cp\u003eUnless otherwise specified in the following experiments, each sample was determined in triplicate, and the average value was taken.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.3 GC-TCD\u0026amp;FID analysis\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eMoisture determination conditions\u003c/strong\u003e: HP-PLOT Q gas chromatography column (30 m \u0026times; 0.53 mm \u0026times; 40\u0026micro;m); TCD detector temperature: 270\u0026deg;C; injection port temperature: 260\u0026deg;C; split injection mode with a split ratio of 10:1; injection volume: 1.0 \u0026mu;L; carrier gas: helium (\u0026ge; 99.999%), constant flow mode, flow rate: 6.0 mL/min; make-up gas: helium, flow rate: 10 mL/min; reference flow rate: 25 mL/min.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e1,2-propanediol, glycerol, and menthol determination conditions\u003c/strong\u003e: HP-INNOWax gas chromatography column (30 m \u0026times; 0.25 mm \u0026times; 0.25\u0026micro;m); FID detector temperature: 270\u0026deg;C; injection port temperature: 260\u0026deg;C; split injection mode with a split ratio of 25:1; injection volume: 1.0 \u0026micro;L; carrier gas: helium (\u0026ge; 99.999%),\u0026nbsp;constant flow mode: flow rate set at 1.2 mL/min; air flow rate at 400 mL/min; hydrogen flow rate at 40 mL/min; make-up gas is helium with a flow rate of 25 mL/min.\u003c/p\u003e\n\u003cp\u003eHeating Procedure: initiate at a temperature of 110 ℃ and maintain for 1 minute. Subsequently, increase the temperature at a rate of 10 ℃/min to reach 150 ℃, where it is held for 2 minutes. Further elevate the temperature at a rate of 20 ℃/min to 240 ℃ and maintain for 5 minutes.\u003c/p\u003e"},{"header":"3. Results and Discussion","content":"\u003cdiv id=\"Sec12\" class=\"Section2\"\u003e \u003ch2\u003e3.1 Selection of Sample Morphology\u003c/h2\u003e \u003cp\u003eFor flake samples, the influence of two sample morphologies (4mm\u0026times;4mm cuboid and powdered) on the extraction efficiency of target compounds using methanol as the extraction solvent under oscillatory extraction conditions was investigated. The results (Table\u0026nbsp;\u003cspan refid=\"Tab1\" class=\"InternalRef\"\u003e1\u003c/span\u003e) indicate that the extraction efficiency for 1,2-propanediol is approximately 6% higher in the cuboid form compared to the powdered form; for menthol, the extraction efficiency is approximately 9% higher in the cuboid form; however, for glycerol, the extraction efficiency is approximately 2% lower in the cuboid form. This may be because 1,2-propanediol has a boiling point of 187\u0026deg;C, and menthol has a boiling point of 216\u0026deg;C. During the pulverization process of tobacco samples, friction generates heat, causing more volatilization of 1,2-propanediol and menthol, which have lower boiling points, resulting in higher extraction efficiency of 1,2-propanediol and menthol from cuboid tobacco samples compared to powdered samples. In contrast, glycerol has a boiling point of 290\u0026deg;C and is difficult to volatilize, making powdered tobacco samples more favorable for its extraction.The extraction efficiency for moisture is comparable between the cuboid and powdered forms. Taking these considerations into account, the 4mm\u0026times;4mm cuboid morphology was selected as the sample morphology for flake samples.\u003c/p\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab1\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 1\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eEffects of sample morphology on extraction efficiency of target compounds(n\u0026thinsp;=\u0026thinsp;3)\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"6\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c6\" colnum=\"6\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e \u003cp\u003eSample No.\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003eSample morphology\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003eWater content (%)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c4\"\u003e \u003cp\u003e1,2-propanediol content (%)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c5\"\u003e \u003cp\u003eGlycerol content (%)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c6\"\u003e \u003cp\u003eMenthol content (%)\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\" morerows=\"1\" rowspan=\"2\"\u003e \u003cp\u003e1 #\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e4 mm\u0026times;4 mm cuboid\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e6.26\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e2.75\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e17.82\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e1.30\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003ePowdered\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e6.29\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e2.58\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e18.16\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e1.18\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\" morerows=\"1\" rowspan=\"2\"\u003e \u003cp\u003e2 #\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e4 mm\u0026times;4 mm cuboid\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e6.43\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e3.59\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e17.21\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e2.11\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003ePowdered\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e6.47\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e3.37\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e17.48\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e1.92\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec13\" class=\"Section2\"\u003e \u003ch2\u003e3.2 Selection of extraction solvent\u003c/h2\u003e \u003cp\u003eThe extraction efficiencies of target compounds using isopropanol, anhydrous ethanol, and methanol were investigated. The results (Table\u0026nbsp;\u003cspan refid=\"Tab2\" class=\"InternalRef\"\u003e2\u003c/span\u003e) indicate that anhydrous ethanol exhibits significantly higher extraction efficiency for glycerol compared to methanol and isopropanol. Furthermore, anhydrous ethanol demonstrates slightly higher extraction efficiencies for water, 1,2-propanediol, and menthol than methanol and isopropanol. Therefore, anhydrous ethanol was selected as the extraction solvent.\u003c/p\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab2\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 2\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eEffects of extraction solvent on extraction efficiency of target compounds(n\u0026thinsp;=\u0026thinsp;3)\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"6\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c6\" colnum=\"6\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e \u003cp\u003eSample No.\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003eExtraction solvent\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003eWater content (%)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c4\"\u003e \u003cp\u003e1,2-propanediol content (%)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c5\"\u003e \u003cp\u003eGlycerol content (%)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c6\"\u003e \u003cp\u003eMenthol content (%)\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\" morerows=\"2\" rowspan=\"3\"\u003e \u003cp\u003e1#\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eIsopropanol\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e6.21\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e2.69\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e15.16\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e1.24\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eAnhydrous ethanol\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e6.26\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e2.75\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e17.82\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e1.30\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eMethanol\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e6.23\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e2.71\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e17.11\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e1.27\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\" morerows=\"2\" rowspan=\"3\"\u003e \u003cp\u003e2#\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eIsopropanol\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e6.38\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e3.54\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e14.82\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e2.05\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eAnhydrous ethanol\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e6.43\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e3.59\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e17.21\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e2.11\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eMethanol\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e6.36\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e3.55\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e16.62\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003e2.07\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec14\" class=\"Section2\"\u003e \u003ch2\u003e3.3 Comparative determination of alcoholic compounds in the same standard solution using FID and TCD detectors\u003c/h2\u003e \u003cp\u003eWang Kang\u003csup\u003e\u003cspan citationid=\"CR19\" class=\"CitationRef\"\u003e19\u003c/span\u003e\u003c/sup\u003e et al. established a GC-TCD method for the simultaneous detection of water, nicotine, 1,2-propanediol, glycerol, triacetin, and menthol in aerosol from heat-not-burn cigarettes. Kensheng Ye\u003csup\u003e\u003cspan citationid=\"CR20\" class=\"CitationRef\"\u003e20\u003c/span\u003e\u003c/sup\u003e et al. also developed a GC-TCD method for the simultaneous determination of water, 1,2-propanediol, nicotine, and glycerol in tobacco materials for heat-not-burn cigarettes. To assess the feasibility of using the TCD detector for routine quality control measurements of 1,2-propanediol and glycerol in tobacco and tobacco products, standard working solutions of 1,2-propanediol, glycerol, and menthol were determined using both the established method and the literature method\u003csup\u003e\u003cspan citationid=\"CR19\" class=\"CitationRef\"\u003e19\u003c/span\u003e\u003c/sup\u003e. The results (Table\u0026nbsp;\u003cspan refid=\"Tab3\" class=\"InternalRef\"\u003e3\u003c/span\u003e) show that the detection values (peak areas) of the TCD detector for 1,2-propanediol, glycerol, menthol, and 1,3-butanediol are significantly lower than those of the FID detector. The results indicate that the FID exhibits higher detection sensitivity towards these four alcoholic compounds, rendering it more suitable for routine quality control testing.\u003c/p\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab3\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 3\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eDetermination of alcohols in the same standard solution by FID detector and TCD detector\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"5\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e \u003cp\u003eTarget compound\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003eConcentration (mg/mL)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003eFID detection value (Peak area)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c4\"\u003e \u003cp\u003eTCD detection value (Peak area)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c5\"\u003e \u003cp\u003ePeak area ratio (FID/TCD)\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e1,2-Propanediol\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e3.432\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e764.2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e54.4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e14.1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eGlycerol\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e16.354\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e2785.3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e211.6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e13.2\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eMenthol\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e3.291\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e1621.6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e35.6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e45.7\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e1,3-Butanediol\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e3.013\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e794.9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e46.2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e17.2\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec15\" class=\"Section2\"\u003e \u003ch2\u003e3.4 Methodological validation\u003c/h2\u003e \u003cp\u003eReference was made to the \"Guiding Principles for Analytical Method Validation\" numbered 9101 in the \"General Technical Requirements\" section of the 2020 edition, Part IV, of the \"Pharmacopoeia of the People's Republic of China\"\u003csup\u003e\u003cspan citationid=\"CR21\" class=\"CitationRef\"\u003e21\u003c/span\u003e\u003c/sup\u003e to investigate the linearity, detection limit, quantitation limit, recovery, and reproducibility of the method.\u003c/p\u003e \u003cdiv id=\"Sec16\" class=\"Section3\"\u003e \u003ch2\u003e3.4.1 Linearity, detection limit, and quantitation limit\u003c/h2\u003e \u003cp\u003eThe concentration ranges of the compounds in the standard working solutions were set to basically cover the content ranges of the target compounds commonly found in tobacco and tobacco products. The detection limit and quantitation limit were determined using the signal-to-noise ratio method: by comparing the signals measured from known low-concentration samples with the signals measured from blank samples, the contents of the corresponding target compounds at signal-to-noise ratios of 3:1 and 10:1 were used to determine the detection limit and quantitation limit, respectively.\u003c/p\u003e \u003cp\u003eLinearity assessment of the water determination method: Seven levels of water standard working solutions were measured according to the water determination conditions to establish a water standard curve (with the ratio of water concentration to isopropanol concentration as the abscissa and the ratio of water peak area to isopropanol peak area as the ordinate). The linearity of the standard curve was evaluated using the correlation coefficient.\u003c/p\u003e \u003cp\u003eLinearity assessment of the determination methods for 1,2-propanediol, glycerol, and menthol: Six levels of standard working solutions were measured according to the determination conditions for 1,2-propanediol, glycerol, and menthol, and standard curves for 1,2-propanediol, glycerol, and menthol were established respectively (with the ratio of target compound concentration to internal standard concentration as the abscissa and the ratio of target compound peak area to internal standard peak area as the ordinate). The linearity of the standard curves was evaluated using the correlation coefficient.\u003c/p\u003e \u003cp\u003eThe results (Table\u0026nbsp;\u003cspan refid=\"Tab4\" class=\"InternalRef\"\u003e4\u003c/span\u003e) indicate that the standard curves for the four target compounds exhibit good linearity, with low detection limits and quantitation limits, and high method sensitivity. The recovery rates for the three alcohols range from 94.49\u0026ndash;102.30%, with an RSD of 1.25\u0026ndash;2.32%. Additionally, the linearity of the standard curves mentioned above surpasses the research findings of Wang, L. Q., et al., regarding the determination of alcohols.\u003csup\u003e\u003cspan citationid=\"CR22\" class=\"CitationRef\"\u003e22\u003c/span\u003e\u003c/sup\u003e\u003c/p\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab4\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 4\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eLinear parameters, detection limits, and quantitation limits of four target compounds\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"6\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c6\" colnum=\"6\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e \u003cp\u003eTarget compound\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003eLinear range (mg/mL)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003eRegression equation\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c4\"\u003e \u003cp\u003eCorrelation coefficient\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c5\"\u003e \u003cp\u003eDetection limit (mg/mL)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c6\"\u003e \u003cp\u003eQuantitation limit (mg/mL)\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eWater\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e0.2\u0026thinsp;~\u0026thinsp;9.0\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003ey\u0026thinsp;=\u0026thinsp;15.671x\u0026thinsp;+\u0026thinsp;5.205\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e0.9987\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e0.32\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e0.97\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e1,2-propanediol\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e0.02\u0026thinsp;~\u0026thinsp;3.2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003ey\u0026thinsp;=\u0026thinsp;223.927x \u0026minus;\u0026thinsp;1.643\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e0.9999\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e0.21\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e0.58\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eGlycerol\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e0.1\u0026thinsp;~\u0026thinsp;16.0\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003ey\u0026thinsp;=\u0026thinsp;171.310x \u0026minus;\u0026thinsp;7.484\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e0.9999\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e0.15\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e0.67\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eMenthol\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e0.02\u0026thinsp;~\u0026thinsp;3.2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003ey\u0026thinsp;=\u0026thinsp;495.415x \u0026minus;\u0026thinsp;0.612\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e0.9999\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e0.04\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e0.21\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec17\" class=\"Section3\"\u003e \u003ch2\u003e3.4.2 Recovery and reproducibility of the method\u003c/h2\u003e \u003cp\u003eRecovery assessment method: A known amount of a standard compound of known purity was precisely added to a sample with a known content (such that the sum of the added amount and the original content remained within the linear range of the standard curve). The sample was then measured according to the established method. The recovery was calculated by dividing the difference between the measured value and the original content in the sample by the added amount of the standard compound. Seven parallel measurements were performed.\u003c/p\u003e \u003cp\u003eThe results (Table\u0026nbsp;\u003cspan refid=\"Tab5\" class=\"InternalRef\"\u003e5\u003c/span\u003e) demonstrate that the method exhibits high accuracy and excellent reproducibility.\u003c/p\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab5\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 5\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eRecovery and precision of the method(n\u0026thinsp;=\u0026thinsp;7)\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"6\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c6\" colnum=\"6\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e \u003cp\u003eTarget compound\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003eOriginal content/mg\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003eSpike amount/mg\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c4\"\u003e \u003cp\u003eMeasured range/mg\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c5\"\u003e \u003cp\u003eRecovery range/%\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c6\"\u003e \u003cp\u003eRSD/%\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eWater\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e93.9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e70.6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e67.9\u0026thinsp;~\u0026thinsp;72.6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e96.13\u0026thinsp;~\u0026thinsp;102.83\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e2.33\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e1,2-Propanediol\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e41.25\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e30.3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e29.3\u0026thinsp;~\u0026thinsp;31.0\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e96.80\u0026thinsp;~\u0026thinsp;102.30\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e2.32\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eGlycerol\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e267.3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e200.2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e191.6\u0026thinsp;~\u0026thinsp;198.6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e95.69\u0026thinsp;~\u0026thinsp;99.22\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e1.25\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eMenthol\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e19.5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e14.1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e13.3\u0026thinsp;~\u0026thinsp;13.9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e94.49\u0026thinsp;~\u0026thinsp;98.33\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e1.36\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003c/div\u003e \u003c/div\u003e \u003cdiv id=\"Sec18\" class=\"Section2\"\u003e \u003ch2\u003e3.5 Sample analysis\u003c/h2\u003e \u003cp\u003eThe tobacco material samples were analyzed according to the established method. The gas chromatogram for water, 1,2-propanediol, glycerol, and menthol determination is presented in Fig.\u0026nbsp;\u003cspan refid=\"Fig3\" class=\"InternalRef\"\u003e3\u003c/span\u003e. The results indicate that the peaks of each target compound and internal standard are sharp and well-separated.\u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003cp\u003eThe measurement results for the 16 tobacco material samples are presented in Table\u0026nbsp;\u003cspan refid=\"Tab6\" class=\"InternalRef\"\u003e6\u003c/span\u003e. Specifically, samples numbered 1#~3# represent, 4#~5# for sheet-type heated tobacco products (produced via dry papermaking), 6#~13# for filament-type heated tobacco products (manufactured through the thick slurry method), and 14#~16# for filament-type conventional cigarettes. This is shown in Fig.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003e, the average moisture content follows the order: reconstituted tobacco leaves for filament-type heated tobacco products (thick slurry method)\u0026thinsp;\u0026gt;\u0026thinsp;reconstituted tobacco leaves for filament-type conventional cigarettes\u0026thinsp;\u0026gt;\u0026thinsp;reconstituted tobacco leaves for sheet-type mentholated heated tobacco products\u0026thinsp;\u0026gt;\u0026thinsp;reconstituted tobacco leaves for sheet-type heated tobacco products (dry papermaking). The average 1,2-propanediol content in reconstituted tobacco leaves for sheet-type mentholated heated tobacco products is significantly higher than that in other sample types. The average glycerol content in reconstituted tobacco leaves for filament-type conventional cigarettes is considerably lower than in other types. Notably, there is considerable variation in the menthol content among different samples of reconstituted tobacco leaves for sheet-type mentholated heated tobacco products. The component content variations are relatively small for both reconstituted tobacco leaves for sheet-type heated tobacco products (dry papermaking) and filament-type conventional cigarettes, with coefficient of variation (CV) values ranging from 0.8\u0026ndash;7.2%. The CVs for glycerol content across all four types of tobacco material samples are also low, falling within the range of 3.1\u0026ndash;6.7%. However, the CVs for moisture and 1,2-propanediol content in reconstituted tobacco leaves for filament-type heated tobacco products (thick slurry method) are relatively high, at 23.5% and 63.9%, respectively.\u003c/p\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab6\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 6\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eAnalysis results of 16 tobacco materials\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"7\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c6\" colnum=\"6\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c7\" colnum=\"7\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e \u003cp\u003eNo.\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003eSample state\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003eType\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c4\"\u003e \u003cp\u003eWater content/%\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1,2-Propanediol content/%\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c6\"\u003e \u003cp\u003eGlycerol content/%\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c7\"\u003e \u003cp\u003eMenthol content/%\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e1#\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eSheet-like\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003eReconstituted tobacco leaf for menthol Heated tobacco products\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e6.26\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e2.75\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e17.82\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e \u003cp\u003e1.30\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e2#\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e6.43\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e3.59\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e17.20\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e \u003cp\u003e2.10\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e3#\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e7.90\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e3.25\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e16.17\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e \u003cp\u003e1.73\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e4#\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003eReconstituted tobacco leaf for heated tobacco products (Dry papermaking)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e5.19\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e0.82\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e17.77\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e \u003cp\u003e-\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e5#\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e5.70\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e0.74\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e18.57\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e \u003cp\u003e-\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e6#\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eFilamentous\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003eReconstituted tobacco leaf for heated tobacco products (Dense slurry method)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e6.27\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e0.77\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e18.51\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e \u003cp\u003e-\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e7#\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e7.54\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e0.71\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e18.42\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e \u003cp\u003e-\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e8#\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e10.96\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e0.34\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e16.75\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e \u003cp\u003e-\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e9#\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e10.37\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e0.23\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e16.47\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e \u003cp\u003e-\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e10#\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e6.93\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e0.24\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e16.41\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e \u003cp\u003e-\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e11#\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e6.72\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e0.23\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e16.04\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e \u003cp\u003e-\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e12#\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e6.29\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e0.24\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e16.18\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e \u003cp\u003e-\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e13#\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e7.28\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e0.95\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e18.70\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e \u003cp\u003e-\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e14#\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003eReconstituted tobacco leaf for traditional cigarettes\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e6.92\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e0.56\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e0.67\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e \u003cp\u003e-\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e15#\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e6.96\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e0.53\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e0.71\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e \u003cp\u003e-\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e16#\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e6.85\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e0.49\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e0.65\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e \u003cp\u003e-\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec19\" class=\"Section2\"\u003e \u003ch2\u003e3.6 Establishment of quality inspection technical standards for Enterprises\u003c/h2\u003e \u003cp\u003eUsing the established method, quality inspection technical standards were formulated by Sichuan China Tobacco Industrial Co., Ltd. for routine quality control testing of water, 1,2-propanediol, glycerol, and menthol in various tobacco and tobacco products. The standards have been in operation for one year with satisfactory results.\u003c/p\u003e \u003c/div\u003e"},{"header":"4. Conclusion","content":"\u003cp\u003eA GC-TCD\u0026amp;FID method for the simultaneous determination of moisture, 1,2-propanediol, glycerol, and menthol contents in tobacco materials was established. (1) The standard curves of the four components exhibited good linearity (correlation coefficient R\u0026thinsp;\u0026gt;\u0026thinsp;0.995 for moisture and R\u0026thinsp;\u0026gt;\u0026thinsp;0.999 for the three alcohols), high accuracy (recovery rates of 94.49\u0026ndash;102.83%), good reproducibility (RSD of 1.25\u0026ndash;2.33%), and high sensitivity (limits of detection for water was 0.32mg/mL and limits of detection for the three alcohols ranging from 0.04mg/mL to 0.21mg/mL). (2) The established method reduces the original process of twice pre-treatments\u0026thinsp;+\u0026thinsp;twice GC analyses to only once sample pre-treatment\u0026thinsp;+\u0026thinsp;once GC analysis, reducing the sample pre-treatment time and GC analysis time by approximately 50%, and using absolute ethanol instead of methanol as the solvent reduces the consumption of chemical reagents and experimental consumables by approximately 50%. (3) The established method has been in operation for one year in the quality inspection technical standards of related products at China Tobacco Sichuan Industrial Co., Ltd., with good results.\u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003e \u003ch2\u003eCompeting interests:\u003c/h2\u003e \u003cp\u003eThe authors declare no competing interests.\u003c/p\u003e \u003c/p\u003e\u003ch2\u003eAuthor Contribution\u003c/h2\u003e\u003cp\u003eWriting-original draft preparation, Yaping Ma; Data curation, Qinlin Xiao and Li Li; Methodology, Juan Yang, Jing Wen and Yuyang Deng; Project administration, Jia Guo Xixiang Zhang and Wu Wen ; Writing-review and editing, Yi Shen. All authors have read and agreed to the published version of the manuscript.\u003c/p\u003e\u003ch2\u003eData Availability\u003c/h2\u003e\u003cp\u003eThe data for this study will be made available upon request from the corresponding author.\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\u003cli\u003e\u003cspan\u003eGaworski, C. L., Oldham, M. J. \u0026amp; Coggins, C. R. E. Toxicological considerations on the use of propylene glycol as a humectant in cigarettes. \u003cem\u003eToxicology\u003c/em\u003e \u003cb\u003e269\u003c/b\u003e(1): 54\u0026ndash;66. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1016/j.chroma.2017.07.079(2010\u003c/span\u003e\u003cspan address=\"10.1016/j.chroma.2017.07.079(2010\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e).\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eGaworski, C. L., Oldham, M. J. \u0026amp; Coggins, C. R. E. 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An isotope dilution ultra high performance liquid chromatography-tandem mass spectrometry method for the simultaneous determination of sugars and humectants in tobacco products. \u003cem\u003eJ. Chromatogr. A\u003c/em\u003e. \u003cb\u003e1514\u003c/b\u003e, 95\u0026ndash;102. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1016/j.chroma.2017.07.079\u003c/span\u003e\u003cspan address=\"10.1016/j.chroma.2017.07.079\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e (2017).\u003c/span\u003e\u003c/li\u003e\u003c/ol\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"scientific-reports","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scirep","sideBox":"Learn more about [Scientific Reports](http://www.nature.com/srep/)","snPcode":"","submissionUrl":"","title":"Scientific Reports","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Scientific Reports","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"Reconstituted tobacco leaves, GC‒TCD\u0026FID method, Glycerol, 1,2‒Propanediol, Menthol, Water","lastPublishedDoi":"10.21203/rs.3.rs-6111429/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-6111429/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eMoisture, 1,2-propanediol, and glycerol are crucial quality indicators that often require simultaneous determination during the production and application of novel tobacco products and reconstituted tobacco leaves. However, the inability to accurately measure these components simultaneously poses a significant challenge. This study innovatively proposes the use of GC-TCD\u0026amp;FID for the simultaneous determination of moisture, 1,2-propanediol, glycerol and menthol. The results showed that: (1) The standard curves of the four components had good linearity ( the correlation coefficient R\u003csup\u003e\u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e\u003c/sup\u003e for water\u0026thinsp;\u0026gt;\u0026thinsp;0.995, and R\u003csup\u003e\u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e\u003c/sup\u003e for the three alcohols\u0026thinsp;\u0026gt;\u0026thinsp;0.999), with recoveries ranging from 94.49\u0026ndash;102.83%, repeatability RSD ranging from 1.25\u0026ndash;2.33%, limits of detection for water was 0.32mg/mL and limits of detection for the three alcohols ranging from 0.04mg/mL to 0.21mg/mL. (2) The process of sample pretreatment and GC analysis has been consolidated from two separate steps into one, thereby reducing the workload, the consumption of reagents and time required for both sample pretreatment and GC analysis by about 50%, and anhydrous ethanol instead of methanol was used as the solvent, meeting the requirements for green, low‒carbon, and sustainable development. This method has been validated and applied for one year, meeting the production requirements.\u003c/p\u003e","manuscriptTitle":"Simultaneous determination of moisture, 1,2-propanediol, glycerol and menthol in tobacco products using GC‒TCD\u0026amp;FID in parallel method","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-04-22 07:18:12","doi":"10.21203/rs.3.rs-6111429/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2025-05-14T07:11:39+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-05-01T15:27:02+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-04-30T02:42:45+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"53156634853906866024143167501590323365","date":"2025-04-25T03:57:51+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"240536911388318637748417021461406545924","date":"2025-04-21T15:08:48+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2025-04-21T12:39:37+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2025-04-20T19:39:16+00:00","index":"","fulltext":""},{"type":"submitted","content":"Scientific Reports","date":"2025-04-18T01:43:52+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"scientific-reports","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scirep","sideBox":"Learn more about [Scientific Reports](http://www.nature.com/srep/)","snPcode":"","submissionUrl":"","title":"Scientific Reports","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Scientific Reports","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"ded7e0aa-2fc9-4fa4-bc5b-09d2fff18568","owner":[],"postedDate":"April 22nd, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[{"id":47457256,"name":"Physical sciences/Chemistry"},{"id":47457257,"name":"Physical sciences/Engineering"},{"id":47457258,"name":"Biological sciences/Chemical biology"}],"tags":[],"updatedAt":"2025-07-14T16:02:18+00:00","versionOfRecord":{"articleIdentity":"rs-6111429","link":"https://doi.org/10.1038/s41598-025-09092-x","journal":{"identity":"scientific-reports","isVorOnly":false,"title":"Scientific Reports"},"publishedOn":"2025-07-09 15:57:40","publishedOnDateReadable":"July 9th, 2025"},"versionCreatedAt":"2025-04-22 07:18:12","video":"","vorDoi":"10.1038/s41598-025-09092-x","vorDoiUrl":"https://doi.org/10.1038/s41598-025-09092-x","workflowStages":[]},"version":"v1","identity":"rs-6111429","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-6111429","identity":"rs-6111429","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}