HPLC determination of danazol in rat plasma and uterus tissue

Objective:To develop a method for determination of danazol in rat plasma and uterus tissue.Methods: Plasma and uterus tissue samples were prepared by precipitating protein in plasma and uterus.The method was per- formed on an Agilent Zorbax Eclipse XDB-C_(18)(250 mm×4.6 mm,5 μm).The mobile phase consisted of metha- nol-water(78:22).The detector was set at 284 nm and the flow rate was 1.0 mL·min~(-1).Results:The linear calibratiiv curves were observed in the concentration range of 31.25-2500 ng·mL~(-1)in plasma and 62.5-2500 ng·g~(-1)in uterus tissue,respectively.The lower limits of quantification were 31.25 ng·mL~(-1)and 62.5 ng·g~(-1), respectively.The relative recoveries were more than 90%.Conclusion:The method is accurate and convenient.It can be used to determine danazol for studying their preclinical pharmacokinetics.