Multifaceted Investigation of Curvularia lunata in Rice: Isolation, Identification, and Grain Spot Disease Pathogenicity

Multifaceted Investigation of Curvularia lunata in Rice: Isolation, Identification, and Grain Spot Disease Pathogenicity | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 7 May 2025 V1 Latest version Share on Multifaceted Investigation of Curvularia lunata in Rice: Isolation, Identification, and Grain Spot Disease Pathogenicity Authors : Varshini S 0009-0006-0153-3951 [email protected] , Raja J , and AJAYDESOUZA Vincent Paul Raj 0009-0006-8526-0742 Authors Info & Affiliations https://doi.org/10.22541/au.174663417.75126066/v1 273 views 141 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Grain spot disease in rice, caused by Curvularia lunata , poses a significant threat to rice production, leading to substantial yield losses. Aim of the study is to isolate, identify, and evaluate the pathogenicity of C. lunata from infected rice grains in Tamil Nadu, India. The pathogen was isolated using single hyphal tip and spore isolation techniques, followed by morphological and molecular identification. Colonies exhibited rapid growth on Potato Dextrose Agar (PDA), with distinct greyish-brown pigmentation. Light and scanning electron microscopy confirmed the characteristic conidial morphology of C. lunata . Molecular identification using ITS region sequencing confirmed the species identity, with the isolate showing 89% genetic homology to previously reported C. lunata strains. Pathogenicity tests confirmed the fungus as the causal organism of grain spot disease, as inoculated plants developed characteristic lesions, fulfilling Koch’s postulates. Growth analysis on different media revealed PDA as the most suitable for optimal mycelial development and sporulation. The findings emphasize the necessity of early detection and effective management strategies to mitigate C. lunata -induced grain spot disease in rice. Future research should focus on the genetic diversity of C. lunata isolates, host-pathogen interactions, and resistance breeding in rice. Additionally, exploring eco-friendly biocontrol agents and integrated disease management approaches could offer sustainable solutions. This research contributes to the understanding of C. lunata as an important rice pathogen and underscores the need for continued surveillance to prevent potential outbreaks in rice-growing regions worldwide. Supplementary Material File (multifaceted investigation of curvularia lunata in.docx) Download 51.45 MB Information & Authors Information Version history V1 Version 1 07 May 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords curvularia lunata disease management grain spot disease molecular identification pathogenicity Authors Affiliations Varshini S 0009-0006-0153-3951 [email protected] Annamalai University Faculty of Agriculture View all articles by this author Raja J Annamalai University Faculty of Agriculture View all articles by this author AJAYDESOUZA Vincent Paul Raj 0009-0006-8526-0742 Annamalai University Faculty of Agriculture View all articles by this author Metrics & Citations Metrics Article Usage 273 views 141 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Varshini S, Raja J, AJAYDESOUZA Vincent Paul Raj. Multifaceted Investigation of Curvularia lunata in Rice: Isolation, Identification, and Grain Spot Disease Pathogenicity. Authorea . 07 May 2025. DOI: https://doi.org/10.22541/au.174663417.75126066/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. 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