Effect of Chen's Neiyi Pill on Expression of Matrix Metalloproteinases in Ectopic Endometrium of Endometriosis Rats

Objective To evaluate the effect of Chen's Neiyi Pill(CNP) on expression of the matrix metalloproteinases 2 and 9(MMP-2 and MMP-9) in ectopic endometrium of endometriosis rats,and to explore its possible therapeutic mechanism.Methods SD rats were randomized into pseudo operation group,model group,danazol group(36 mg·kg-1·d-1),and high-,middle-and low-dose CNP groups(in the dose of 7.2,3.6,and 1.8 g·kg-1·d-1 respectively).Rat models of endometriosis were established.After modeling,the medication groups were given the corresponding drugs according to the experimental design,and the pseudo operation group and the model group were given distilled water for 4 weeks.One week after the last medication,ectopic endometrium of endometriosis rats was taken out for the observation of MMP-2 and MMP-9 expression.Results MMP-2 and MMP-9 expression levels were higher in the model group than those in CNP groups and danazol group,the difference being significant(P0.01).MMP-2 and MMP-9 expression levels were lower in high-dose CNP group than those in middle-and low-dose CNP groups and danazol group,the difference being significant(P0.05 or P0.01).The differences of MMP-2 and MMP-9 expression levels were insignificant between middle-and low-dose CNP groups and danazol group(P0.05).Conclusion The therapeutic mechanism of CNP for endometriosis is probably related with the inhibition of the increase of MMP-2 and MMP-9 expression levels in ectopic endometrium of endometriosis rats.