Fibulin-1 Improves Caerulein-Induced Acute Pancreatitis In Vitro by Inhibiting Ferroptosis.

Acute pancreatitis is an inflammatory disease of the pancreas that is often accompanied by various complications, which seriously impair patient health. Fibulin-1 (FBLN1) is an extracellular matrix (ECM) protein that plays a crucial role in several cellular processes. Previous quantitative analyses of plasma protein profiles of severe acute pancreatitis patients and healthy volunteers have shown that the expression of FBLN1 is decreased in severe acute pancreatitis patients. However, the regulatory roles of FBLN1 in patients with acute pancreatitis have not been fully investigated. In this study, FBLN1 was found to be expressed at lower levels in acute pancreatitis. Moreover, FBLN1 inhibited Caerulein-triggered inflammatory injury in AR42J cells. It was also found that FBLN1 suppressed Caerulein-triggered ferroptosis in AR42J cells, and that FBLN1 could activate the NRF2/HO-1 pathway. In conclusion, FBLN1 may relieve Caerulein-induced acute pancreatitis in AR42J cells by inhibiting ferroptosis, providing insights on the use of FBLN1 in acute pancreatitis treatment.