Combinatorial Approach of poly-D, L-lactide Encapsulated Quercetin, Piperlongumine and Curcumin Inhibits Tumour Growth in Balb/c Mice, the Colon Cancer Model

Abstract Colon cancer has become an extreme danger to human lives due to high frequency and mortality around the world. The natural formulations based on the nanoparticles have been found promising in terms of cost, procedure and side effects. In this study, for the first time, three natural molecules- quercetin, piperlongumine and curcumin were encapsulated in biodegradable poly-D,L-lactide nanoparticles to check their anticancer potential against the colon cancer (DMH-DSS). The pure molecules, blank and loaded nanoparticles were given to the colon cancer model (BALB/c mice) and evaluated the anticancer potential of the nano-formulations. Ficus benghalensis (Banyan) coated nanoparticles maintained the detectable concentration of quercetin, piperlongumine and curcumin in colon even in liver, kidney and serum after 8 h of administration. In case of sugar coated NPs, quercetin, piperlongumine and curcumin were detected in colon, kidney and liver. Further, it was found that the coating of nanoparticles surface with leaf extract and dextrose sugar enhanced the potential of the formulation. This newly synthesized formulation can have the potential to be explored further as anticancer drug against the colon cancer.