Inhibitory effects of Jiawei Foshousan on angiogenesis of endometriosis in rats model and its mechanism

Aim To explore the effect and the mechanism of Jiawei Foshousan on angiogenesis of endometriosis(EMS)in rats.Methods The rat model of EMS was established by surgical implant of the endometrial tissue which belong to its body.We explored microvessels density(MVD)by the immunohistochemical test as well as the expression of vascular endothelial growth factor(VEGF)protein and endostatin protein in ectopic endometriotic tissue by the immunohistochemical test and western blot.The level of secretion of prostaglandin E2(PGE2)in the blood serum and ectopic endometriotic tissue was measured by radioimmunoassay(RIA).Results Jiawei Foshousan 0.045,0.09,0.18 g·kg-1 effectively inhibited the volume of ectopic endometriotic tissue,Jiawei Foshousan 0.09,0.18 g·kg-1 reduced MVD,the expression of VEGF protein and the level of secretion of PGE2 and Jiawei Foshousan 0.18 g·kg-1 increased the expression of endostatin protein.Conclusions Jiawei Foshousan can reduce the degree of angiogenesis of EMS in the rats model,and its mechanisms might be associated with the regulation of estrogen and the expression of VEGF and endostatin.