Evaluation of Highly Conserved Burkholderia pseudomallei Outer Membrane Proteins as Protective Antigens Against Respiratory Melioidosis

Evaluation of Highly Conserved Burkholderia pseudomallei Outer Membrane Proteins as Protective Antigens Against Respiratory Melioidosis | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Evaluation of Highly Conserved Burkholderia pseudomallei Outer Membrane Proteins as Protective Antigens Against Respiratory Melioidosis Alexander J. Badten, Susana Oaxaca-Torres, Ritwika S. Basu, Matthieu G. Gagnon, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6549424/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 06 Aug, 2025 Read the published version in npj Vaccines → Version 1 posted 13 You are reading this latest preprint version Abstract Burkholderia pseudomallei (Bpm), the etiological agent of melioidosis, lacks approved vaccines. However, several candidate vaccines have conferred protection in animal models. Interestingly, some of these vaccines have been shown to induce cross-protective immunity against the closely related species B. mallei. This led us to explore whether there exists a subset of antigens that are conserved in other pathogenic Burkholderia species which could potentially serve as components of a pan-Burkholderia vaccine. We assessed the proteome of Bpm and identified several antigens that are conserved in the B. cepacia complex. To evaluate these antigens, we intranasally immunized mice with three proteins (OmpA1, OmpA2, Pal) coupled to an immunogenic gold nanoparticle (AuNP) platform, resulting in the induction of robust Th1/Th2-balanced responses and mucosal immunity. Animals immunized with AuNP-OmpA1 or AuNP-OmpA2 exhibited protection against a lethal Bpm respiratory challenge, which supports the idea that these antigens could be candidates for a cross-protective vaccine. Biological sciences/Microbiology/Bacteria Biological sciences/Microbiology/Bacteriology Biological sciences/Microbiology/Vaccines gold nanoparticle melioidosis Burkholderia pseudomallei nanovaccines vaccine platform reverse vaccinology Full Text Additional Declarations No competing interests reported. Supplementary Files SupplementaryFiguresFinal.pdf SupplementaryData11.xlsx Cite Share Download PDF Status: Published Journal Publication published 06 Aug, 2025 Read the published version in npj Vaccines → Version 1 posted Editorial decision: Revision requested 17 Jun, 2025 Reviews received at journal 11 Jun, 2025 Reviewers agreed at journal 04 Jun, 2025 Reviewers agreed at journal 04 Jun, 2025 Reviewers agreed at journal 04 Jun, 2025 Reviewers agreed at journal 03 Jun, 2025 Reviews received at journal 20 May, 2025 Reviewers agreed at journal 12 May, 2025 Reviewers agreed at journal 08 May, 2025 Reviewers invited by journal 08 May, 2025 Editor assigned by journal 08 May, 2025 Submission checks completed at journal 30 Apr, 2025 First submitted to journal 28 Apr, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6549424","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":455464878,"identity":"26364ee6-c661-4668-83d0-dcd598d44ba5","order_by":0,"name":"Alexander J. 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