Effect of bone marrow mesenchymal stem cells on cisplatin induced cytotoxicity in parotid gland of rats (Histological and Ultrastructural studies)

Objective: the current study was implemented to assess the therapeutic efficacy of bone marrow mesenchymal stem cells on parotid glands following cytotoxicity induced by cisplatin administration in rats. Materials: and Methods: The study employed a sample of 36 adult male Sprague-Dawley rats. The samples were categorized into two groups. Group I: The control group (6 rats) received 0.5ml phosphate-buffered saline. Group II: The experimental group was divided into two subgroups; subgroup (A), referred to as the cisplatin group, (15 rats) was administered a solitary intraperitoneal injection of the cisplatin medication at a dosage of 10 mg/kg. Subgroup (B) BMMSc group (15 rats) was administered the same dose of cisplatin as subgroup A. On day three following cisplatin administration, 5x10 5 BMMSCs suspended in 0.5 ml of PBS were injected intraparotid. After cisplatin injection and BMMSc administration rats were sacrificed according to different time intervals (7 and 21 days) for histological and ultrastructural analysis. Results: : stem cell treated group exhibited superior histological and ultrastructural characteristics. Conclusion: This investigation proved that BMMSCs can improve the histological and ultrastructural changes of parotid gland induced by cisplatin administration.