Methods for the analysis of leukemic infiltration within the cerebrospinal fluid in acute lymphoblastic leukaemia - A systematic review

Methods for the analysis of leukemic infiltration within the cerebrospinal fluid in acute lymphoblastic leukaemia - A systematic review | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 7 January 2025 V1 Latest version Share on Methods for the analysis of leukemic infiltration within the cerebrospinal fluid in acute lymphoblastic leukaemia - A systematic review Authors : Lavleen Singh 0000-0002-2352-6975 [email protected] , Shraddhanjali Satapathy , Venkateswaran Iyer , Deepali Jain , and Rachna Seth Authors Info & Affiliations https://doi.org/10.22541/au.173621920.06284443/v1 314 views 164 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Central nervous system (CNS) involvement in acute lymphoblastic leukaemia is associated with a poor prognosis, making accurate detection essential for risk stratification and guiding treatment decisions. Cytomorphology of cerebrospinal fluid has been the gold standard for assessing CNS involvement. However, it has limited sensitivity, particularly in low cell counts. Flow cytometry has emerged to be a valuable diagnostic tool with higher sensitivity, particularly in paucicellular samples. Occult CNS disease has emerged as a newer group where the cytomorphology shows no blasts while the flowcytometry records the leukemic blast population. Flow cytometry remains challenging due to the lack of standardised positivity criteria, unclear prognostic implications and management strategies for occult CNS diseases. This systematic review critically examines the methodologies for detecting leukemic infiltration within cerebrospinal fluid, focusing on conventional cytomorphology and multiparameter flow cytometry. In addition, it also addresses key pre-analytical variables, such as cerebrospinal fluid volume, processing time, and total nucleated cell counts. These parameters significantly influence the accuracy of both methods. Future research is required to establish uniform diagnostic guidelines and assess the clinical relevance of occult CNS disease in acute lymphoblastic leukaemia. Supplementary Material File (main manuscript.docx) Download 83.91 KB Information & Authors Information Version history V1 Version 1 07 January 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords all hematology/oncology pathology Authors Affiliations Lavleen Singh 0000-0002-2352-6975 [email protected] All India Institute of Medical Sciences New Delhi View all articles by this author Shraddhanjali Satapathy All India Institute of Medical Sciences New Delhi View all articles by this author Venkateswaran Iyer All India Institute of Medical Sciences New Delhi View all articles by this author Deepali Jain All India Institute of Medical Sciences New Delhi View all articles by this author Rachna Seth All India Institute of Medical Sciences New Delhi View all articles by this author Metrics & Citations Metrics Article Usage 314 views 164 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Lavleen Singh, Shraddhanjali Satapathy, Venkateswaran Iyer, et al. Methods for the analysis of leukemic infiltration within the cerebrospinal fluid in acute lymphoblastic leukaemia - A systematic review. Authorea . 07 January 2025. DOI: https://doi.org/10.22541/au.173621920.06284443/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . Format Please select one from the list RIS (ProCite, Reference Manager) EndNote BibTex Medlars RefWorks Direct import Tips for downloading citations document.getElementById('citMgrHelpLink').addEventListener('click', function() { popupHelp(this.href); return false; }); $(".js__slcInclude").on("change", function(e){ if ($(this).val() == 'refworks') $('#direct').prop("checked", false); $('#direct').prop("disabled", ($(this).val() == 'refworks')); }); View Options View options PDF View PDF Figures Tables Media Share Share Share article link Copy Link Copied! Copying failed. 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