Improvement of nucleotide content of Cordyceps tenuipes by Schisandra chinensis: fermentation process optimization and application prospects

Improvement of nucleotide content of Cordyceps tenuipes by Schisandra chinensis: fermentation process optimization and application prospects | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Improvement of nucleotide content of Cordyceps tenuipes by Schisandra chinensis: fermentation process optimization and application prospects Anni Li, Qiang Zhu, Yuting Li, Lu Yang, Zhihao Chen, Xiang Zhou, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4127978/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 13 May, 2024 Read the published version in Archives of Microbiology → Version 1 posted 4 You are reading this latest preprint version Abstract Nucleotides are important components and the main indicators for judging Cordyceps quality. To increase the nucleoside content of Cordyceps tenuipes for enhancing the efficacy activity. In this paper, the mixed fermentation process of Schisandra chinensis and C. tenuipes was systematically studied, and it was proposed that the fermentation products aqueous extract (S-ZAE) had antioxidant activity and anti-AChE ability. Herein, the results of a single factor showed that S. chinensis , yeast extract, inoculum amount, and pH had significant effects on nucleotide synthesis, and these four factors were used as the factor of response surface optimization. The results of response surface optimization were 3% glucose, 0.25% KH 2 PO 4 , 2.1% yeast extract, and S. chinensis 0.49% (m/v), the optimal fermentation conditions were 25℃, inoculum 5.8% (v/v), pH 3.8, 6 d. The yield of total nucleotides in the scale-up culture was 0.64 ± 0.027 mg/mL, which was 10.67 times that before optimization, and S-ZAE has good antioxidant and anti-AChE activities. This fermentation method has the advantage of industrialization, and its fermentation products have the potential to become good functional foods or natural therapeutic agents. Cordyceps tenuipes Schisandra chinensis Fermentation process Nucleotide AChE Antioxidant Full Text Additional Declarations No competing interests reported. Supplementary Files Graphicalabstract.pdf Cite Share Download PDF Status: Published Journal Publication published 13 May, 2024 Read the published version in Archives of Microbiology → Version 1 posted Editorial decision: Revision requested 20 Mar, 2024 Editor assigned by journal 20 Mar, 2024 Submission checks completed at journal 20 Mar, 2024 First submitted to journal 19 Mar, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4127978","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":281738870,"identity":"aced3eb5-a727-45eb-bb4b-4b48b24e952c","order_by":0,"name":"Anni Li","email":"","orcid":"","institution":"Jiangsu Ocean University","correspondingAuthor":false,"prefix":"","firstName":"Anni","middleName":"","lastName":"Li","suffix":""},{"id":281738871,"identity":"059cefc1-0251-4ae3-8e3f-73706acb4052","order_by":1,"name":"Qiang Zhu","email":"","orcid":"","institution":"Jiangsu Ocean 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Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"Cordyceps tenuipes, Schisandra chinensis, Fermentation process, Nucleotide, AChE, Antioxidant","lastPublishedDoi":"10.21203/rs.3.rs-4127978/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4127978/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eNucleotides are important components and the main indicators for judging \u003cem\u003eCordyceps\u003c/em\u003e quality. To increase the nucleoside content of \u003cem\u003eCordyceps tenuipes\u003c/em\u003e for enhancing the efficacy activity. In this paper, the mixed fermentation process of \u003cem\u003eSchisandra chinensis\u003c/em\u003e and \u003cem\u003eC. tenuipes\u003c/em\u003e was systematically studied, and it was proposed that the fermentation products aqueous extract (S-ZAE) had antioxidant activity and anti-AChE ability. Herein, the results of a single factor showed that \u003cem\u003eS. chinensis\u003c/em\u003e, yeast extract, inoculum amount, and pH had significant effects on nucleotide synthesis, and these four factors were used as the factor of response surface optimization. The results of response surface optimization were 3% glucose, 0.25% KH\u003csub\u003e2\u003c/sub\u003ePO\u003csub\u003e4\u003c/sub\u003e, 2.1% yeast extract, and \u003cem\u003eS. chinensis\u003c/em\u003e 0.49% (m/v), the optimal fermentation conditions were 25℃, inoculum 5.8% (v/v), pH 3.8, 6 d. The yield of total nucleotides in the scale-up culture was 0.64\u0026thinsp;\u0026plusmn;\u0026thinsp;0.027 mg/mL, which was 10.67 times that before optimization, and S-ZAE has good antioxidant and anti-AChE activities. This fermentation method has the advantage of industrialization, and its fermentation products have the potential to become good functional foods or natural therapeutic agents.\u003c/p\u003e","manuscriptTitle":"Improvement of nucleotide content of Cordyceps tenuipes by Schisandra chinensis: fermentation process optimization and application prospects","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-03-22 20:14:58","doi":"10.21203/rs.3.rs-4127978/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2024-03-20T09:38:07+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2024-03-20T09:22:34+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2024-03-20T06:32:53+00:00","index":"","fulltext":""},{"type":"submitted","content":"Archives of Microbiology","date":"2024-03-19T06:57:48+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"archives-of-microbiology","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"aomi","sideBox":"Learn more about [Archives of Microbiology](https://www.springer.com/journal/203)","snPcode":"203","submissionUrl":"https://submission.nature.com/new-submission/203/3","title":"Archives of Microbiology","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false}}],"origin":"","ownerIdentity":"ee428e2b-ba1b-418b-83ce-d3b9e29b5a19","owner":[],"postedDate":"March 22nd, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[],"tags":[],"updatedAt":"2024-06-17T13:04:52+00:00","versionOfRecord":{"articleIdentity":"rs-4127978","link":"https://doi.org/10.1007/s00203-024-03988-4","journal":{"identity":"archives-of-microbiology","isVorOnly":false,"title":"Archives of Microbiology"},"publishedOn":"2024-05-13 13:04:52","publishedOnDateReadable":"May 13th, 2024"},"versionCreatedAt":"2024-03-22 20:14:58","video":"","vorDoi":"10.1007/s00203-024-03988-4","vorDoiUrl":"https://doi.org/10.1007/s00203-024-03988-4","workflowStages":[]},"version":"v1","identity":"rs-4127978","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4127978","identity":"rs-4127978","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}