Antibody Production Techniques and Antibody Types: Evolution, Methodologies, and Applications

Antibody Production Techniques and Antibody Types: Evolution, Methodologies, and Applications | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 11 August 2025 V1 Latest version Share on Antibody Production Techniques and Antibody Types: Evolution, Methodologies, and Applications Author : Shobith Suresh 0000-0001-6336-1418 [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.175494695.59237185/v1 171 views 133 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract The generation of antibodies is a cornerstone of modern biomedical research, diagnostics, and therapy. This review provides an extensive overview of antibody production techniques, from classical polyclonal methods to cutting-edge recombinant and display technologies. It also discusses diverse antibody types, including natural immunoglobulin isotypes, monoclonal antibodies, engineered fragments such as single-chain variable fragments (scFv), nanobodies, and bispecific formats. Each antibody production technique is examined in terms of molecular principles, protocols, historical significance, advantages, limitations, and applications. The integration of polymerase chain reaction (PCR) in antibody gene cloning and engineering has notably accelerated discovery and optimization. This combined analysis equips researchers with a technical and conceptual framework to select and implement antibody technologies tailored to research and clinical demands. Supplementary Material File (antibodies.pdf) Download 439.09 KB Information & Authors Information Version history V1 Version 1 11 August 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords antibody engineering antibody production monoclonal antibodies nanobodies phage display polyclonal antibodies polymerase chain reaction recombinant antibodies scfv therapeutic antibodies Authors Affiliations Shobith Suresh 0000-0001-6336-1418 [email protected] Jawaharlal Nehru Centre for Advanced Scientific Research View all articles by this author Metrics & Citations Metrics Article Usage 171 views 133 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Shobith Suresh. Antibody Production Techniques and Antibody Types: Evolution, Methodologies, and Applications. Authorea . 11 August 2025. DOI: https://doi.org/10.22541/au.175494695.59237185/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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